Abstract
Phosphoglucose isomerase negative mutant of mucoid Pseudomonas aeruginosa accumulated relatively higher concentration of fructose 1,6-bisphosphate (Fru-1,6-P2) when mannitol induced cells were incubated with this sugar alcohol. Also the toluene-treated cells of fructose 1,6-bisphosphate aldolase negative mutant of this organism produced Fru-1,6-P2 from fructose 6-phosphate in presence of ATP, but not from 6-phosphogluconate. The results together suggested the presence of an ATP-dependent fructose 6-phosphate kinase (EC 2.7.1.11) in mucoid P. aeruginosa.
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Abbreviations
- ALD:
-
Fru-1,6-P2 aldolse
- DHAP:
-
dihydroxyacetone phosphate
- F6P:
-
fructose 6-phosphate
- G6P:
-
glucose 6-phosphate
- Gly3P:
-
glyceraldehyde 3-phosphate
- KDPG:
-
2-keto 3-deoxy 6-phosphogluconate
- PFK:
-
fructose 6-phosphate kinase
- PGI:
-
phosphoglucose isomerase
- 6PG:
-
6-phosphogluconate
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Banerjee, P.C. Accumulation of fructose 1,6-bisphosphate in mutant cells of mucoid Pseudomonas aeruginosa as an evidence of phosphofructokinase activity. Arch. Microbiol. 144, 405–407 (1986). https://doi.org/10.1007/BF00409892
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DOI: https://doi.org/10.1007/BF00409892