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Synthesis of 99mTc-Radiolabeled Uridine as a Potential Tumor Imaging Agent

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Radiochemistry Aims and scope

Abstract

Uridine, a pyrimidine nucleoside essential for the synthesis of RNA and biomembranes, was radiolabeled with 99mTc to obtain a potential tumor imaging agent. The maximal radiochemical yield of about 96.5%, as determined by paper chromatography and instant thin-layer chromatography, was reached under the following optimum conditions: 1 mg of uridine, 20 μg of SnCl2·2H2O as reducing agent, 20 mg of mannitol as a stabilizer, and pH 8. 99mTc-uridine is stable in vitro at room temperature for up to 6 h post labeling. The biodistrbution study in tumor-bearing mice shows high target-to-nontarget ratio. These results match with the high docking score of the complex on uridine phosphorylase enzyme. 99mTc-uridine shows promise as a tumor imaging agent.

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Authors and Affiliations

  1. Hot Laboratories Centre, Atomic Energy Authority, P.O. Box 13759, Cairo, Egypt

    H. M. Talaat, I. T. Ibrahim, N. A. Bayomy & N. Farouk

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  1. H. M. Talaat
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  2. I. T. Ibrahim
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  3. N. A. Bayomy
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  4. N. Farouk
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Correspondence to H. M. Talaat.

Additional information

Published in Russian in Radiokhimiya, 2018, Vol. 60, No. 1, pp. 49–54.

The text was submitted by the authors in English.

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Talaat, H.M., Ibrahim, I.T., Bayomy, N.A. et al. Synthesis of 99mTc-Radiolabeled Uridine as a Potential Tumor Imaging Agent. Radiochemistry 60, 51–57 (2018). https://doi.org/10.1134/S1066362218010095

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  • DOI: https://doi.org/10.1134/S1066362218010095

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