Elsevier

FEBS Letters

Volume 166, Issue 1, 23 January 1984, Pages 62-66
FEBS Letters

α2 High molecular mass cysteine proteinase inhibitor: HMrα2-CPI: An inhibitor of human liver cathepsin H as probed by kinetic study

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Abstract

HMrα2CPI was found to be an inhibitor of human liver cathepsin H by the measurement of the dissociation constant (Ki), the association rate constant (k1) and the dissociation rate constant (k−1) between the enzyme and the inhibitor. These data suggest that this protein-proteinase inhibitor can play a physiological role in the regulation of free cathepsin H.

Keywords

α2-Cysteine proteinase inhibitor
Cathepsin H
Kinetic study
Physiological role

Abbreviations

HMrα2CPI, α2 cysteine proteinase inhibitor, high-Mr
C.H, cathepsin H (EC 3.4.22.16)
BzArgNNap, N-benzoyl-DL-arginine-2-naphthylamide hydrochloride
ArgNNap, L-arginine-2-naphthylamide hydrochloride
LeuNNap, L-leucine-2-naphthylamide hydrochloride
ArgNMec, L-arginine-4-methyl-7-coumarylamide
E 64, L-trans-epoxysuccinyl-leucyl-amido(4-guanidino)butane
pI, isoelectric point
DTT, 1,4-dithioerythreitol

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