Skip to main content
Log in

Pancreatic islet cell suspensions from newborn rats; different preparation procedures, viability and (pro)insulin biosynthesis

  • Published:
Experientia Aims and scope Submit manuscript

Summary

Islet cell suspensions were prepared from neonatal rat pancreatic islets. While mechanical disintegration results in a higher yield, cells prepared by trypsin treatment appear to be better preserved. Trypsin treatment of pancreatic islets during the cell preparation procedure does not influence the stimulation by glucose of (pro)insulin biosynthesis in freshly isolated cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Petersson, B., Gunnarsson, R., Hellerström, C., and Anderson, A., Acta diabet. lat.10 (1973) 725.

    Article  Google Scholar 

  2. Krause, U., Puchinger, H., and Wacker, A., Hormone metab. Res.5 (1973) 725.

    Google Scholar 

  3. Lernmark, Å., Diabetologia10 (1974) 431.

    Article  CAS  PubMed  Google Scholar 

  4. Kromann, H., Christy, M., Egeberg, J., Lernmark, Å., Nerup, J., and Richter-Olesen, H., Med. Biol.58 (1980) 322.

    CAS  PubMed  Google Scholar 

  5. Dobersen, M.J., Scharff, J.E., Ginsberg-Fellner, F., and Notkins, A.L., New Engl. J. Med.303 (1980) 1493.

    Article  CAS  PubMed  Google Scholar 

  6. Dobersen, M.J., Scharff, J.E., Ginsberg-Fellner, F., Diabetologia21 (1981) 264, abstr. 127.

    Google Scholar 

  7. Deleers, M., Ruysschaert, J.M., and Malaisse, W.J., Biochem. biophys. Res. Commun.98 (1981) 255.

    Article  CAS  PubMed  Google Scholar 

  8. Ono, J., Takaki, R., and Fukuma, M., Endocr. jap.24 (1977) 265.

    Article  CAS  PubMed  Google Scholar 

  9. Lernmark, Å., Fedn Proc.34 (1975) 235.

    Google Scholar 

  10. Niki, A., Niki, H., and Ozawa, T., FEBS Lett.80 (1977) 5.

    Article  CAS  PubMed  Google Scholar 

  11. Lernmark, Å., Nielsen, D.A., Nielsen, O., and Larsen, J.K., Uppsala J. med. Sci.86 (1981) 119.

    Article  CAS  Google Scholar 

  12. Lernmark, Å., Nathans, A., and Steiner, D.F., J. Cell Biol.71 (1976) 606.

    Article  CAS  PubMed  Google Scholar 

  13. Grankvist, K., Lernmark, Å., and Täljedal, I.-B., Biochem. J.162 (1977) 19.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Jahr, H., Schröder, D., Ziegler, B., Ziegler, M., and Zühlke, H., Eur. J. Biochem.110 (1980) 499.

    Article  CAS  PubMed  Google Scholar 

  15. Zucker, P., and Logothetopoulos, J., Diabetes24 (1975) 194.

    Article  CAS  PubMed  Google Scholar 

  16. Lernmark, Å., Kanatsuna, T., Patzelt, C., Diakoumis, K., Carroll, R., Rubenstein, A.H., and Steiner, D.F., Diabetologia19 (1980) 445.

    Article  CAS  PubMed  Google Scholar 

  17. Pipeleers, D.G., and Pipellers-Marichal, M.A., Diabetologia20 (1981) 645.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Investigations were carried out as a part of the ‘HFR Diabetes mellitus and Fettstoffwechselstörungen’ supported by the Ministry of Health of the GDR.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schröder, D., Hehmke, B. & Zühlke, H. Pancreatic islet cell suspensions from newborn rats; different preparation procedures, viability and (pro)insulin biosynthesis. Experientia 39, 210–212 (1983). https://doi.org/10.1007/BF01958906

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01958906

Keywords

Navigation