ALBERT

Description: Nonalcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disorder worldwide with no curative therapy. The aim of this study was to investigate the hepatoprotective effects of a novel Trihoney against biochemical and histological manifestations of NAFLD in hypercholesterolemic rabbits. Methodology. Forty-eight male New Zealand white (NZW) rabbits were grouped into normal diet (C), normal diet with 0.6 g/kg/day of Trihoney (C + H), 1% cholesterol diet (HCD), 1% cholesterol diet with 0.3 g/kg/day of Trihoney (HCD + H1), 1% cholesterol diet with 0.6 g/kg/day of Trihoney (HCD + H2), and 1% cholesterol diet with 2 mg/kg/day of atorvastatin (HCD + At.). Animals were sacrificed after 12 weeks of treatment. Serum lipids and liver function test (LFT) were measured prior to and at the endpoint of the experiment for total cholesterol (TC), low-density lipoprotein (LDL-c), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), and total bilirubin (T. Bil.). Liver was processed for histopathology study. Liver homogenate was analysed for oxidative stress parameters: superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA). Results. Lipid analysis approved the induction of hypercholesterolemia. A significant elevation (p

Description: Gases such as nitric oxide (NO) and carbon monoxide (CO) play important roles both in normal physiology and in disease. Recent studies have shown that hydrogen sulfide (H2S) protects neurons against oxidative stress and ischemia-reperfusion injury and attenuates lipopolysaccharides (LPS) induced neuroinflammation in microglia, exhibiting anti-inflammatory and antiapoptotic activities. The gas H2S is emerging as a novel regulator of important physiologic functions such as arterial diameter, blood flow, and leukocyte adhesion. It has been known that multiple factors, including oxidative stress, free radicals, and neuronal nitric oxide synthesis as well as abnormal inflammatory responses, are involved in the mechanism underlying the brain injury after subarachnoid hemorrhage (SAH). Based on the multiple physiologic functions of H2S, we speculate that it might be a promising, effective, and specific therapy for brain injury after SAH.

Description: We present results on the first principle study of the elastic constants and the phonon dispersion of EuFe2As2 at zero pressure. The ground-state energy calculations were performed within Density Functional Theory (DFT) and the generalized gradient approximation using the pseudopotential method with plane-wave basis sets. The projector augmented-wave (PAW) pseudopotentials were used in our calculation. The open source code QUANTUM ESPRESSSO was used with its pseudopotential database. The study on the elastic constants at zero pressure was a clear indication that the compound is mechanically stable, and the phonon dispersion study also indicated that the compound is dynamically stable. The elastic constants and mechanical properties also led to the conclusion that the compound is ductile and anisotropic.

Description: Despite the success of antibiotic discovery, infectious diseases remain the second leading source of death worldwide, while the resistance to antibiotics is among the significant problems in the twenty-first century. Medicinal plants are very rich in phytochemicals which can be structurally optimized and processed into new drugs. Nigeria enjoys a diverse collection of medicinal plants, and joint research has ascertained the efficacy of these plants. Plants such as guava (Psidium guajava), ginger (Zingiber officinale), neem (Azadirachta indica), and moringa (Moringa oleifera) have been found to exhibit broad range of antimicrobial activities. Studies on Nigerian plants have shown that they contain alkaloids, polyphenols, terpenes, glycosides, and others with possible therapeutic potentials. The antimicrobial activities of some new compounds such as alloeudesmenol, hanocokinoside, orosunol, and 8-demethylorosunol, identified from medicinal plants in Nigeria, are not yet explored. Further investigation and optimization of these compounds will facilitate the development of new sets of pharmacologically acceptable antimicrobial agents. This review study revealed the efficacy of medicinal plants as an alternative therapy in combating and curtailing the development and survival of multidrug-resistant pathogens coupled with the toxic effects of some antibiotics. Due to enormous therapeutic possibilities buried in medicinal plants, there is a need for more research into unique fingerprints and novel compounds that can provide cure to the neglected tropical diseases (NTDs) of humans and animals facing Africa, especially Nigeria.

Description: This study aims to determine the quality of olive oils (Picholine variety) from the traditional oil mills in different Moroccan cities by means of physicochemical characterization and chemical compositions. All samples of olive oil were collected from traditional oil mills. Physicochemical analyses of free fatty acid (FFA), iodine value (IV), saponification value (SV), specific extinction at 232 and 270 (E232, E270), chlorophyll content, carotenoid content, fatty acids (FAs), and total phytosterols composition were performed with respect to the International Olive Council (IOC) standards. These oils were revealed to be rich in unsaturated fatty acids (UFAs): C18 : 1, C18 : 2, and C18 : 3, and that the total phytosterols content ranged between 142.68 and 208.72 mg per 100 g of oil. Also, the chlorophyll contents, for most of the studied samples, are less than 2 mg/kg, while the carotenoid content varied between 0.13 and 0.63 mg/kg. These results, along with the physicochemical assays, helped classify the oils studied into three categories: extra virgin, virgin, and ordinary virgin olive oils. These results confirm that the conditions under which olive oils are collected, pressed, and stored influence the quality of the oil produced. Therefore, there is a need to inform producers about the correct practices and techniques for storage, processing, and conservation of oils to better improve the quality of the final product.

Description: The current upsurge in resistance to conventional antibiotics, as well as high cost of orthodox medical treatment, called for the use of medicinal plants as an alternative therapy. This research was aimed at determining the antibacterial activity of Artocarpus heterophyllus seed extracts (Jackfruit as it is locally called) in the treatment of diarrhoea. Ethanolic and hexanolic seed crude extracts of the plant were screened for antidiarrhoeal activity against bacteria isolated from clinical samples (methicillin-resistant and susceptible Staphylococcus aureus, multidrug-resistant Pseudomonas aeruginosa, ciprofloxacin-resistant Salmonella typhimurium, and third-generation cephalosporin-resistant Escherichia coli). Plant phytochemical screening was conducted using standard methods. The antibacterial activity was carried out using the agar well diffusion method and compared to the standard antibiotics ceftriaxone and vancomycin. The minimum inhibitory concentration was determined by the microbroth dilution method, whereas the minimum bactericidal concentration was determined by plating out from microtitre plates with no visible growth. The results of phytochemical screening revealed the presence of tannins, flavonoids, reducing sugars, cardiac glycosides, saponins, and steroids from the prepared crude extracts. The ethanolic and hexanolic extracts had activity on multidrug-resistant Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and methicillin-susceptible Staphylococcus aureus with the mean and standard error zone of inhibition that ranged from 8.5 ± 0.5 to 16.5 ± 0.25 mm; however, the extracts were found not to have activity on resistant E. coli and Salmonella typhimurium. The ethanolic crude extract had the lowest MIC and MBC values of 31.25 and 125 mg/ml, respectively, compared to the hexane extract which had the MIC and MBC values of 62.50 and 250 mg/ml, respectively. This provides the evidence for its usage as an alternative herbal remedy for the treatment of diarrhoea caused by susceptible and methicillin-resistant Staphylococcus aureus and multidrug resistant Pseudomonas aeruginosa.

Description: The aim of this study was to evaluate the application of cocoa pulp as an adjunct for malt in beer production. The cocoa pulp was analyzed for humidity, proteins, lipids, sugars, total soluble solids, organic acids, and minerals. A study was carried out to reduce the cocoa pulp viscosity by enzymatic depectinization, making its use viable in beer production. The cocoa pulp showed relevant quantities of compounds important in fermentation, such as sugars, acids, and minerals. In fermentation using the adjunct, the proportions of pulp used were 10, 30, and 49%. A significant difference was found between the adjunct and all-malt worts. The 30% cocoa pulp concentration as an adjunct for malt in the fermentation medium contributed the most to the fermentative performance of the yeasts at both 15 and 22°C based on the consumption of apparent extract (°Plato), ethanol production, and cellular growth.

Description: Aim. To evaluate the prevalence of impacted teeth in Saudi patients and compare between male and female subjects. Method. This cross-sectional study comprised of Saudi patients who attended dental clinics in major hospitals in the Eastern Province of Saudi Arabia. Patients’ dental records and panoramic radiographs were reviewed retrospectively. Impacted teeth excluding third molars and spaces occupied by primary, permanent, and transmigrated teeth were recorded from panoramic radiographs. The Pearson chi-squared test was performed to determine gender differences regarding impacted teeth and spaces occupied by other teeth. Results. The study included radiographs of 539 patients with a mean age of 23.3 ± 10.8 years. Seventy-one patients (13.2%) had at least one impacted tooth. The total number of impacted teeth was 115 in the sample, out of which 91 (79.1%) were in the upper arch and 24 (20.8%) in the lower arch. Fifty-eight maxillary canines (50.4%) were impacted making them the most commonly impacted teeth, followed by 21 upper second premolars (18.2%) and 14 lower second premolars (12.2%). More females (70.7%) than males (29.3%) had impacted teeth (P=0.82). Of 61 spaces occupied, 35 (57.4%) were occupied by permanent teeth, 24 (39.3%) by primary teeth, and 2 (3.3%) by transmigrated teeth. Greater proportions of spaces were occupied in female than male participants (P〉0.05). Conclusion. There was a high prevalence of impacted teeth in Saudi patients. The canines were the most commonly impacted teeth followed by the second premolars. Females demonstrated a higher occurrence of impacted teeth than males. Early detection of impacted teeth can help prevent malocclusion and maintain a healthy dentition.

Description: Land use changes have long been considered among many factors responsible for physical and chemical soil degradation. This study was conducted to evaluate land use and land cover (LULC) changes and their cumulative effects over 30 years (from 1989 to 2019) on the current physical and chemical properties of soils in the Tejibara watershed, Ethiopia. Image analysis and LULC classifications were performed using ERDAS IMAGINE 2014 and ArcGIS 10.4 software, respectively. For the determination of soil properties, four land use types (natural forest, eucalyptus plantation, cultivated, and grazing lands) and two soil depths (0–20 and 20–40 cm) were used. Triplicate composite soil samples were collected from each land use type and soil depths. For the determination of physical (texture and bulk density) and chemical soil properties such as electrical conductivity (EC), organic matter (OM), total nitrogen (TN), and available phosphorus (AP), standard laboratory procedures were employed. The image analysis results for all of the years studied show that cultivated lands have increased in area at the expense of forest and grazing lands. Silt content, clay content, AP, and pH were significantly affected by land use as the main effect while the interaction effects of soil depth and land use were significant for total N and OM only. The highest (10.1 mg/kg) and the lowest (4.9 mg/kg) AP contents were observed in the forest and the grazing lands, respectively. Soil total N content was highest in the forest lands (0.32%) and lowest in cultivated lands (0.06%). Concerning OM content, the highest (11.0%) and the lowest (0.8%) values were recorded in the forest and cultivated lands, respectively. Generally, this study showed that land use changes have reduced the areal coverage by forest and grazing lands and have negatively affected the soil properties. This implies that land use change without soil fertility measures that are appropriate to the area could cause enhanced land degradation and thereby reduce the productivity of the study area soils.

Description: Geladas are the most distinctive of Ethiopian endemic mammals, representing the last extant species of primate genus that have a very restricted distribution in the northern Ethiopian plateau. The activity budget and feeding ecology of geladas (Theropithecus gelada obscurus) were studied around Abogedam Church, Ethiopia, from May to October 2014, encompassing dry and wet seasons. The scan sampling method was applied to collect behavioural data on the identified band. Activity scans were collected at 15-minute intervals for up to five minutes duration from 0700 to 1730 h. The activity recorded for each individual was the first activity that lasts for five seconds. During each scan, individuals were recorded as performing activities: feeding, moving, resting, playing, aggression, grooming, sexual activity, and others. On average, geladas devoted 57.19% feeding, 14.82% resting, 14.92% moving, 4.83% playing, 2.53% aggression, 4.14% grooming, 1.23% sexual activity, and 0.34% other activities such as vocalization, defecation, and urination. Forty-one plant species were consumed by geladas that belonged to 18 families of which 53.66% were grasses. This study provides basic information on further studies and motivates conservationists to plan the management of unprotected areas at the vicinity of agricultural lands where such endemic animals dwell.