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  • 1
    Electronic Resource
    Electronic Resource
    Sea bass (Dicentrarchus labrax L.) vitellogenin. I-Induction, purification and partial characterization
    Amsterdam : Elsevier
    Comparative Biochemistry and Physiology -- Part B: Biochemistry and 107 (1994), S. 205-216 
    Details
    ISSN: 0305-0491
    Keywords: Dicentrarchus labrax ; Vitellogenin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0305-0491(94)90041-8
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  • 2
    Electronic Resource
    Electronic Resource
    Sea bass (Dicentrarchus labrax L.) vitellogenin. II-Validation of an enzyme-linked immunosorbent assay (ELISA)
    Amsterdam : Elsevier
    Comparative Biochemistry and Physiology -- Part B: Biochemistry and 107 (1994), S. 217-223 
    Details
    ISSN: 0305-0491
    Keywords: Dicentrarchus labrax L ; Vitellogenin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0305-0491(94)90042-6
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  • 3
    Electronic Resource
    Electronic Resource
    Pattern of sea bass oocyte development after ovarian stimulation by LHRHa (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 41 (1992), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The cyclic pattern of oocyte development in the sea bass, Dicentrarchus labrax L., was studied after induction of spawning by two injections, 24 h apart, of a luteinizing hormone releasing-hormone analog (LHRHa) administered at the end of vitellogenesis. The first difference in the developmental stage of the ovary and in the size-frequency distribution of oocytes between the LHRHa treated group and the control group, was detected 32 h after the first injection, the LHRHa group showing a higher proportion of the 900 μm diameter oocyte class (maturing oocytes) (P〈0.01). At 48 h LHRHa-treated females showed an increase in the 1000 and 1100 μm classes (maturing oocyte and ovulated eggs) (P〈0.01) and at 72 h these females exhibited a bimodal pattern, reaching the highest proportions in the 1100 (27.4%) and the 600 (14.7%) μm classes (ovulated eggs and advanced vitellogenic oocytes, respectively). Bimodal distributions were present in 80% of the LHRHa-treated females. Once oocyte final maturation was triggered by LHRHa the time needed for ovulation was about 48 h and the interval between consecutive ovulations and spawnings seemed to be 48–72 h.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1992.tb02723.x
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  • 4
    Electronic Resource
    Electronic Resource
    Influence of rearing temperature during the larval and nursery periods on growth and sex differentiation in two Mediterranean strains of Dicentrarchus labrax (2005)
    Oxford, UK; Malden, USA : Blackwell Science Ltd
    Journal of fish biology 67 (2005), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: European sea bass Dicentrarchus labrax of the north-western (NW) and south-eastern (SE) Mediterranean Sea strains were exposed to different temperatures (13, 17 or 21° C) during the larval rearing (11–51 days post hatching, dph) or nursery periods (55–95 dph), in order to examine the effects of temperature on sex differentiation and subsequent growth during the first year of life. Higher growth was observed during exposure to higher temperatures, but fish of the NW strain exposed to 13 or 17° C during larval rearing exhibited compensatory growth once exposure to the lower temperatures finished, and as a result their final size at 300 dph was similar or greater to the group exposed to 21° C. Fish exposed to 17° C during the nursery period also had similar size to fish exposed to 21° C after 300 days of rearing, but the fish exposed to 13° C remained significantly smaller (ANOVA, n = 55–100, P 〈 0·05). There were significant differences in the sex ratio among the fish exposed to different temperatures during the two periods of rearing, with high temperature (21° C) resulting in a significantly higher percentage of males in the population, both in the NW (ANOVA, n = 2, P 〈 0·04) and SE populations (ANOVA, n = 2, P 〈 0·01). The masculinization effect of high temperature was significantly stronger during the larval rearing stage, both in the NW (ANOVA, n = 2, P 〈 0·005) and SE populations (ANOVA, n = 2, P 〈 0·01). None of the temperature manipulations could produce 100% females, suggesting that there is a part of the genetic component in sex differentiation which is not labile to environmental influence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0022-1112.2005.00766.x
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  • 5
    Electronic Resource
    Electronic Resource
    Relative potency of the forms of GnRH and their analogs on LH release in sea bass (2003)
    Oxford, UK : Blackwell Science Ltd
    Journal of fish biology 63 (2003), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The in vivo and in vitro potency of native and modified forms of gonadotropin releasing hormone (GnRH) to release luteotropic hormone (LH) was studied in sea bass Dicentrarchus labrax in particular the hypothalamic fish-specific sea bream GnRH form (sbGnRH) and the general mesoencephalic form chicken GnRH-II (cGnRH-II). The potencies of the natives and their analogs (GnRHas) were referred to that of [D-Ala6, Pro9Net]-mGnRHa (LHRHa) at equivalent doses. Analogs of the native peptides [D-Arg6, Pro9Net]-cGnRH-II, [D-Ala6, Pro9Net]-cGnRH-II, [D-Trp6, Pro9Net]-sbGnRH and [D-Ala6, Pro9Net]-sbGnRH were effective in inducing in vivo LH release (at 15 µg kg−1 body mass), exhibiting longer lasting activity than their corresponding native forms. Injection of sbGnRH and cGnRH-II provoked a small but significant peak of circulating LH at 1·5 h after treatment (a.t.) decreasing down to basal levels at 4 h a.t. [D-Arg6, Pro9Net]-cGnRH-II, [D-Ala6, Pro9Net]-cGnRH-II and [D-Ala6, Pro9Net]-mGnRHa evoked a higher and a more sustained elevation of LH, peaking at 12 h a.t. and returning to basal levels between 48 and 72 h a.t. [D-Trp6, Pro9Net]-sbGnRH and [D-Ala6, Pro9Net]-sbGnRH also induced a significant surge of LH in plasma at 4 h a.t. turning to the basal levels at 24 h a.t. These rises, however, were of less amplitude and duration than the observed after treatment with cGnRH-II analogs and [D-Ala6, Pro9Net]-mGnRHa. The in vitro stimulation of dispersed pituitary cells with the different native and modified forms of GnRH resulted in a dose-dependent increase in the quantity of LH released at 24 h a.t. [D-Arg6, Pro9Net]-cGnRH-II and [D-Ala6, Pro9Net]-cGnRH-II induced the highest response of LH in vitro release followed by salmon GnRH (sGnRH), [D-Ala6, Pro9Net]-mGnRHa and [D-Trp6, Pro9Net]-sbGnRH. The lowest activity was exhibited by sbGnRH. Collectively, the in vitro biological activity (compared by their EC50) can be ordered as follows: [D-Arg6, Pro9Net]-cGnRH-II 〉 [D-Ala6, Pro9Net]-cGnRH-II 〉 sGnRH 〉 [D-Ala6, Pro9Net]-mGnRHa 〉 [D-Trp6, Pro9Net]-sbGnRH 〉 [D-Ala6, Pro9Net]-sbGnRH 〉 cGnRH-II 〉 sbGnRH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1095-8649.2003.00130.x
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  • 6
    Electronic Resource
    Electronic Resource
    Comparative growth performance of diploid and triploid European sea bass over the first four spawning seasons (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 58 (2001), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: During their 3–4 first years of life, triploid sea bass Dicentrarchus labrax grew in a similar fashion to diploids in fork length but more slowly than diploids (P〈0·05) in body weight, even when the diploids reached full sexual maturity. However, from 48–53 months of age triploids exhibited non-significantly higher instantaneous growth rates, and thus when fish were 4 years or older, differences in weight with diploids were no longer apparent, suggesting that triploidy could be of benefit in the culture of large (〉1 kg) sea bass. The condition factor was reduced in both ploidies during the spawning season which took place in winter when the temperature was low. These observations suggest that any growth advantage in triploids, which were functionally sterile, may be offset by unfavourable environmental conditions. Thus, the potential gain of triploid fish, because they do not direct energy to gonadal growth, could not overcome the effects of low temperature on somatic growth, which coincided with the spawning season. This suggests that the low growth of this species during winter is more a consequence of low temperature than of the energetic cost associated with reproduction. On the other hand, the lower hepatosomatic index in triploid females in contrast to diploid females might be indicative of the lack of gonadal oestradiol-mediated hepatic synthesis of vitellogenin. Also, erythrocyte and haematocrit measurements showed an increased nuclear and cellular volume in triploids, but with similar cell numbers to those of diploids, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2001.tb00500.x
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  • 7
    Electronic Resource
    Electronic Resource
    Hormonal regulation of the European sea bass reproductive cycle: an individualized female approach (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 56 (2000), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Fifteen tagged female sea bass Dicentrarchus labrax were sampled weekly from September to April and plasma vitellogenin (VTG), testosterone (T), 17β-estradiol (E2), and two potential maturation inducing steroids (MISs): 17,20β-dihydroxy-4-pregnen-3-one (17,20βP) and 17,20β,21-trihydroxy-4-pregnen-3-one (20βS) assayed. An oocyte sample was obtained via intraovarian cannulation at each sampling time from every female and the stage of development of the most advanced clutch of oocytes determined and related to VTG and hormone plasma levels for each female. The mean number of ovulations per female was 1·75+0·25 when those females that did not present ovulations were excluded and up to 4 ovulations detected in some females. The highest plasma levels of T (c. 6 ng ml-1) were observed during postvitellogenesis and the beginning of maturation while maximum plasma levels of E2 (〉5 ng ml-1) were obtained during late vitellogenesis. VTG plasma levels increased throughout vitellogenesis peaking (c. 2·5 mg ml-1) at postvittelogenesis. For the first time significant changes of plasma progestogens were detected in European sea bass during the sexual cycle. The highest plasma level of 17,20βP (c. 1·1 ng ml-1) was observed during postvitellogenesis while the highest level of 20βS (c. 1·4 ng ml-1) coincided with final maturation. These results suggest that 17,20βP and 20βS play a role in the early and final maturation, respectively, in the European sea bass.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2000.tb02131.x
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  • 8
    Electronic Resource
    Electronic Resource
    Effect of bonito insulin injection on plasma immunoreactive glucagon levels and carbohydrate and lipid metabolism of sea bass (Dicentrarchus labrax)
    Amsterdam : Elsevier
    Comparative Biochemistry and Physiology -- Part A: Physiology 94 (1989), S. 33-36 
    Details
    ISSN: 0300-9629
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0300-9629(89)90779-2
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  • 9
    Electronic Resource
    Electronic Resource
    Annual cycle of plasma lipids in sea bass, Dicentrarchus labrax L.: Effects of environmental conditions and reproductive cycle
    Amsterdam : Elsevier
    Comparative Biochemistry and Physiology -- Part A: Physiology 93 (1989), S. 407-412 
    Details
    ISSN: 0300-9629
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0300-9629(89)90057-1
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  • 10
    Electronic Resource
    Electronic Resource
    Growth and diurnal variations in metabolic parameters in the starved bass, Dicentrarchus labrax, after experimental feeding
    Amsterdam : Elsevier
    Comparative Biochemistry and Physiology -- Part A: Physiology 72 (1982), S. 11-16 
    Details
    ISSN: 0300-9629
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0300-9629(82)90003-2
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