ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
In this study, various dental stem cells were isolated and their mineralization ability wasevaluated. First, we isolated postnatal stem cells from human dental tissues such as dental pulp(DPSCs), periodontal ligament (PDLSCs), periapical follicle (PAFSCs) and mandibular bonemarrow (MBMSCs). Then, we cultured these dental stem cells in the mineralization condition for21 days. Ability of these stem cells to mineralize was evaluated by Alizarin Red staining,quantitative analysis of Alizarin Red, alkaline phosphatase (ALP) activity, and RT-PCR. Thecalcium deposits of PAFSCs were sparsely scattered throughout the adherent layer, whereasMBMSCs and PDLSCs cultures produced extensive sheets of calcified deposits over the entireadherent layer. Quantitative Alizarin Red staining and the ALP activity also were high in MBMSCsand PDLSCs. In the osteogenic differentiation condition, all of dental stem cells showedupregulated expression of the mineralization-related gene. Our findings suggest that isolated DPSCs,PDLSCs, PAFSCs and MBMSCs have the different mineralization abilities
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.129.pdf
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