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Digitale Medien

Starvation in yeast increases non-adaptive mutation (1999)

Marini, A. ; Matmati, N. ; Morpurgo, G.

Springer

Current genetics 35 (1999), S. 77-81

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ISSN: 1432-0983

Schlagwort(e): Key words Adaptive mutations ; 6-N-hydroxylaminopurine ; Saccharomyces cerevisiae

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract The frequency of reversion in a histidine-requiring mutant of Saccharomyces cerevisiae increases about ten-fold in stationary cells during histidine starvation. Histidine starvation enhances a similar frequency of reversion in a tryptophan-requiring mutant. Starvation, therefore, enhances mutation frequencies in a non-adaptive manner. The base analogue 6-N-hydroxylaminopurine (HAP) added prior to plating on medium with limited histidine strongly increases reversion of the histidine mutant. HAP-induced reversion increases further in stationary starving cells with the same kinetics as that which increases spontaneous reversion. Adding HAP to the stationary starving cells does not produce any effect.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s002940050435

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Digitale Medien

The uvsC and uvsE genes of Aspergillus nidulans are not required for the mutagenic repair of UV damage (1998)

Babudri, N. ; Marini, A. ; Matmati, N. ; [weitere]

Springer

Molecular genetics and genomics 259 (1998), S. 130-132

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ISSN: 1617-4623

Schlagwort(e): Key wordsAspergillus nidulans ; uvsC ; uvsE ; UV mutability

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract The uvsC gene of Aspergillus nidulans is a homolog of the RAD51 gene of Saccharomyces cerevisiae. However, with respect to its effects on UV mutagenesis, it differs from the yeast gene, since it seems to be required for UV mutagenesis; however, this conclusion is based only on data from resting conidia. To further clarify the functional role of the uvsC gene, we tested the UV mutability of strains bearing a uvsC mutation in resting as well as in germinating conidia, by the p-fluoro-phenyl-alanine resistance test. We also evaluated the mutability of the uvsE mutant which belongs to the same epistatic group. Our results show that the uvsC and uvsE genes do not have a significant role in the mutagenic UV-repair pathway.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004380050797

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Finding pathway-modulating genes from a novel Ontology Fingerprint-derived gene network (2014)

Qin, T., Matmati, N., Tsoi, L. C., Mohanty, B. K., Gao, N., Tang, J., Lawson, A. B., Hannun, Y. A., Zheng, W. J.

Oxford University Press

In: Nucleic Acids Research

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Publikationsdatum: 2014-10-10

Beschreibung: To enhance our knowledge regarding biological pathway regulation, we took an integrated approach, using the biomedical literature, ontologies, network analyses and experimental investigation to infer novel genes that could modulate biological pathways. We first constructed a novel gene network via a pairwise comparison of all yeast genes’ Ontology Fingerprints—a set of Gene Ontology terms overrepresented in the PubMed abstracts linked to a gene along with those terms’ corresponding enrichment P -values. The network was further refined using a Bayesian hierarchical model to identify novel genes that could potentially influence the pathway activities. We applied this method to the sphingolipid pathway in yeast and found that many top-ranked genes indeed displayed altered sphingolipid pathway functions, initially measured by their sensitivity to myriocin, an inhibitor of de novo sphingolipid biosynthesis. Further experiments confirmed the modulation of the sphingolipid pathway by one of these genes, PFA4 , encoding a palmitoyl transferase. Comparative analysis showed that few of these novel genes could be discovered by other existing methods. Our novel gene network provides a unique and comprehensive resource to study pathway modulations and systems biology in general.

Schlagwort(e): Computational Methods, Miscellaneous/other

Print ISSN: 0305-1048

Digitale ISSN: 1362-4962

Thema: Biologie