Publication Date:
2021-05-19
Description:
One of the mainly valuable consumed colloid protein materials in pharmaceutics, medical and
food industries is Gelatin. Fish gelatin near warm water is similar mammal gelatin. Due to the
amount of catch of yellowfin tuna (Thunnus albacares) use in factory. Fish skin are peel off and
wasted in factory every day. Analysis factors were extracted alkaline method gelatin from skin,
physiochemical and rheological test (amino acid composition (HPLC), electrophoreses, fourier
transform infrared, moisture content, pH, setting point , setting time, melting point and melting
time, color and gelatin yield) with access method of National Iran Standard . Prepared gelatin
nanoparticles from gelatin with desolvation method. Determination of particles size, size
distribution, zeta potential for characterize the surface and morphology .Antibacterial assay with
disc diffusion method and determined MIC (minimum inhibitory concentration) and MBC
(minimum bactericidal concentration) for Esherichia coli, Pseudomonas aeruginosa and
Staphylococcus aureus. For nanoparticles gelatin antibacterial assay (MIC and MBC)
turbidimetry. Statistical processing were linear regression and modeling and forecasting
regression. Albeit antibacterial compared gelatin and gelatin nanoparticles.It produced 0.034g
gelatin for one gram of yellowfin tuna. Yellowfin tuna had higher gelatin content (Proline and
Hydroxyproline) than mammalian gelatin content. SDS-electrophoresis for yellow fin gelatin
showed protein band (α, β, γ) same as mammalian protein band. Fourier transform infrared had
the same spectra for both of them. Factors were pH (6.1), Moisture (8.5%) Setting temperature
and time respectively 4(c) and 60(s) and Melting temperature and time respectively were 50(c)
and 45(s). The color was transparent. The mean size of the gelatin nanoparticles was 132 nm and
PI (polydispersity) and zeta potential were respectively 0.248, -31 MV. pH, speed of addition of
acetone, percent of glutaraldehyde depends on size of nanoparticles gelatin. (Statistically
significant was P〈0.05). Gelatin nanoparticles had antibacterial in Pseudomonas aeruginosa and
Staphylococcus aureus while gelatin had antibacterial Pseudomonas aeruginosa. Nanoparticle
gelatin had higher MIC and MBC than gelatin. Statistically significant was P〈0.05. Antibacterial
assay for gelatin particles has evaluated for foodstuffs packing and it may using drug delivery.
This kind of gelatin is lawand similar mammalian gelatin. Antibacterial properties is more useful
for usage in foodstuffs packing and may using drug delivery.
Description:
Published
Keywords:
Fourier transform infrared
;
Rheological
;
Yellow fin tuna
;
Proline
;
Hydroxyproline
;
Fish
;
Protein
;
Gelatin
;
Amino acids
;
pH
;
Thunnus albacares
;
Extraction
;
Evaluation
;
Pseudomonas aeruginosa
;
Esherichia coli
;
Staphylococcus aureus
Repository Name:
AquaDocs
Type:
Journal Contribution
,
Refereed
Format:
pp.103
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