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  • Articles  (2)
  • Articles: DFG German National Licenses  (2)
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  • Articles  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    TRACKING TOPOISOMERASE ACTIVITY AT THE SINGLE-MOLECULE LEVEL (2005)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Biophysics and Biomolecular Structure 34 (2005), S. 201-219 
    Details
    ISSN: 1056-8700
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology , Physics
    Notes: The recent development of new techniques to manipulate single DNA molecules has opened new opportunities for the study of the enzymes that control DNA topology: the type I and II topoisomerases. These single-molecule assays provide a unique way to study the uncoiling of single supercoiled DNA molecules and the unlinking of two intertwined DNAs. They allow for a detailed characterization of the activity of topoisomerases, including the processivity, the chiral discrimination, and the dependence of their enzymatic rate on ATP concentration, degree of supercoiling, and the tension in the molecule. These results shed new light on the mechanism of these enzymes and their function in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.biophys.34.040204.144433
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  • 2
    Electronic Resource
    Electronic Resource
    Micro‐mechanical measurement of the torsional modulus of DNA (1999)
    Springer
    Genetica 106 (1999), S. 57-62 
    Details
    ISSN: 1573-6857
    Keywords: DNA elasticity ; DNA torsional rigidity ; supercoiling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The torsional modulus C of DNA is determined from the difference between the work of stretching a single overwound molecule and the work done in stretching one underwound by the same number of turns. The value obtained C/kBT=86±10 nm is within the range (75±25 nm) estimated by more indirect methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003772626927
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    Paper (German National Licenses)
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