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  • 1
    ISSN: 1432-1017
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European biophysics journal 7 (1981), S. 265-265 
    ISSN: 1432-1017
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
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  • 3
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    Electronic Resource
    Springer
    European biophysics journal 7 (1981), S. 325-325 
    ISSN: 1432-1017
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
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  • 4
    ISSN: 1432-1017
    Keywords: Cu(II)-complexes ; Peptides ; ESR ; Optical absorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The interaction of Cu(II) with di- and tripeptides each containing phenylalanine, tryptophan or histidine in the amino acid chain has been investigated by means of electron spin resonance (ESR) and optical absorption spectroscopy. Cu(II) complexes of dipeptides and tripeptides exhibit different magnetic and optical parameters. Dipeptide complexes have larger g ∥-values and smaller {A ∥−values than tripeptide complexes. When compared to dipeptide complexes, the d-d band of the central metal ion is blue shifted for tripeptide complexes. There are no significant differences in the behavior of Cu(II) peptide complexes containing phenylalanine or tryptophan. Complexes of histidine containing peptides, however, show modified spectra caused by the participation of the imidazole nitrogen in the coordination to Cu(II). The imidazole nitrogen seems to coordinate in-plane with other coordinating atoms or in an axial position depending on the kind of peptide.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Radiation and environmental biophysics 10 (1973), S. 61-67 
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Summary The NMR spectra of uracil and some of its derivatives have been investigated using deuterated dimethylsulfoxide as a solvent. The spectral positions of the peaks depend on the electron-attracting or -releasing strength of the substituents: rel. to uracil, the peaks were shifted upfleld in the case of the electron-releasing substituents andvice versa. A plot of the electron affinityvs. the H-3 position exhibits a straigth line. Addition of Cu2+ or other metal ions, such as Pe3+, Ni2+, Co2+, Zn2+, and Ag+, results in a splitting of the N-H protons, the distance of which turned out to be a linear relationship to the inverse electronegativity. K+, Na+, Mg2+, NH4 +, Cl−, NO3 −, SO4 2− had no effect at all. The effect of Cu2+ and the substituents as well seems to be mediated by the hydrogen bond formed between the uracil derivative and DMSO.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Radiation and environmental biophysics 11 (1974), S. 203-207 
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Summary Co60-γ-irradiation of an aqueous 0.15 mM 5-iododeoxyuridine (5-IUdR) solution results in an I− absorption band at 226 nm. The absorbance of this band, as obtained from difference spectra (irradiatedvs. unirradiated sample), increases linearly with dose between 200 and 12,000 rad. Smaller doses cannot be detected experimentally; at higher doses, saturation is reached due to the limited amount of iodine present in 5-IUdR. Because of the linear OD-dose relationship this system might be used for radiation dosimetry.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Radiation and environmental biophysics 13 (1976), S. 305-313 
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Electron spin resonance and optical absorption studies have been done in order to investigate the interaction between Cu2+ and aromatic amino acids in aqueous solution at 77 K and at room temperature as well. Depending on the concentration each aromatic amino acid can form two different kinds of complexes with Cu2+ which can be characterized by its ESR pattern. Additional information was obtained from optical d-d and CT transitions.
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  • 8
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 325 (1986), S. 473-475 
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary High-performance liquid chromatography with UV-detection at 254 nm was used for determining ascorbic acid, dehydroascorbic acid, and isoascorbic acid in plasma and erythrocytes obtained from healthy volunteers and from patients with coronary infarct, acute lymphatic leukemia (ALL), and acute myeloic leukemia (AML). Deproteinisation was done by a simple ultrafiltration technique. Dehydroascorbic acid (DHA) was determined after reduction to ascorbic acid with dithiothreitol. The separation system chosen allows the search for the stereoisomer isoascorbic acid. To exclude interfering substances which could coelute with ascorbic or isoascorbic acid, several other compounds present in plasma were investigated including e.g. the aromatic amino acids, glutathione, cysteine, and uric acid. The results reveal a drastic decrease in total vitamin C concentration in plasma and erythrocytes with a concomitant increase in rel. DHA concentration in patients with AML. In ALL patients, both the total vitamin C and rel. DHA concentrations were increased.
    Notes: Zusammenfassung High-performance-liquid-Chromatographie mit UV-Nachweis bei 254 nm wurde zur Bestimmung von Ascorbinsäure, Dehydroascorbinsäure und Isoascorbinsäure in Plasma und Erythrocyten von gesunden Probanden und von Patienten mit Herzinfarkt, akuter lymphatischer Leukämie (ALL) und akuter myeloischer Leukämie (AML) benutzt. Deproteinisierung erfolgte mittels einer einfachen Ultrafiltrationstechnik. Dehydroascorbinsäure wurde nach Reduktion mit Dithiothreitol als Ascorbinsäure bestimmt. Die benutzte Methode erlaubt auch die Bestimmung des Stereoisomers Isoascorbinsäure. Der mögliche Einfluß von im Plasma vorkommenden Substanzen auf die Bestimmung des Vitamin C wurde untersucht. Die Ergebnisse zeigen eine drastische Abnahme der Gesamt-Vitamin C-Konzentration in Plasma und Erythrocyten mit einer parallelen Zunahme der relativen DHA-Konzentration in AML-Patienten. In ALL-Patienten sind sowohl die Gesamt-Vitamin C- als auch die relative DHA-Konzentrationen erhöht.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 317 (1984), S. 129-130 
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary Only LAMMA spectra of negative ions of ascorbic acid and isoascorbic acid exhibit a deprotonated peak. In the case of the radicals Na-ascorbate and K-isoascorbate neither the positive nor the negative ion spectra show a similar peak. Only a peak at mr/z=41 can be detected in the negative ion spectra of both of the radicals. Positive ion spectra exhibit peaks at mr/z =63 (for Na-ASC) and at 95 (for K-Iso-ASC) in addition to the Na+ and K+ peaks in the corresponding radicals. The peaks at 41, 63, and 95 might represent Na and K complexes engulfed in the cyclic structure of the side chain. From the results obtained one can be certain, that both of the radicals are electroneutral.
    Notes: Zusammenfassung Nur die LAMMA-Spektren der negativen Ionen von Ascorbinsäure und Isoascorbinsäure haben einen deprotonierten Peak. Bei den Radikalen Na-Ascorbat und K-Isoascorbat ist dieser weder bei den positiven noch bei den negativen Ionen-Spektren vorhanden. Bei beiden Radikalen kann nur bei mr/z=41 ein Peak im negativen Ionen-Spektrum nachgewiesen werden. Die positiven Ionen-Spektren haben neben den Na+- und K+-Peaks solche bei mr/z=63 (für Na-ASC) und 95 (für K-Iso-ASC). Die Peaks bei 41,63 und 95 stellen wahrscheinlich Na- und K-Komplexe dar, die in der cyclischen Struktur der Seitenkette eingebettet sind. Auf jeden Fall kann aus den erhaltenen Ergebnissen geschlossen werden, daß beide Radikale elektroneutral sind.
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