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  • 1
    Electronic Resource
    Electronic Resource
    Correlation between magnetic susceptibility and distribution of heavy metals in contaminated sea-floor sediments of Hong Kong Harbour (1998)
    Springer
    Environmental geology 36 (1998), S. 77-86 
    Details
    ISSN: 1432-0495
    Keywords: Key words Magnetic susceptibility ; Sea-floor sediments ; Heavy metals ; Hong Kong
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract  Magnetic susceptibility measurements were conducted on 24 vibrocores obtained from an area located off the northeastern coast of Lantau Island in Hong Kong. High intensities of magnetic susceptibility were detected in the uppermost sections of the majority of the cores. Several magnetic parameters measured for one of the cores suggest that the variations in the magnetic characteristics over depth are mainly due to varying concentrations of the magnetic minerals. Since a strong correlation has been found between magnetic susceptibility and the heavy metals Pb, Cu, Zn and Cr, an anthropogenic contamination origin is thought to be the cause. The present study shows that magnetic susceptibility is a fast, inexpensive and non-destructive method for the detection and mapping of contaminated sediments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002540050322
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  • 2
    Electronic Resource
    Electronic Resource
    Synthesis and reactivity of dihydroxoruthenium(IV) complex of tetramesitylporphyrin. Aerobic epoxidation of norbornene by ruthenium porphyrins
    Amsterdam : Elsevier
    Polyhedron 12 (1993), S. 2331-2334 
    Details
    ISSN: 0277-5387
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0277-5387(00)83049-1
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural Evidence for Transport of Protein across the Epithelium of the Rat Caput Epididymidis (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 513 (1987), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1987.tb25099.x
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural correlation of water reabsorption in isolated rat cauda epididymidis (1978)
    Springer
    Cellular and molecular life sciences 34 (1978), S. 485-487 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopic study was made on the water reabsorption of the epithelial cells of the rat cauda epididymidis. It was shown that when the epididymal duct was reabsorbing water at a maximal rate, widely dilated intercellular spaces were seen. It is suggested that the standing gradient model of water reabsorption first proposed for the gall bladder may also operate in the cauda epididymidis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01935943
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  • 5
    Electronic Resource
    Electronic Resource
    A study of bend formation in locally reactivated hamster sperm flagella (1983)
    Springer
    Journal of muscle research and cell motility 4 (1983), S. 625-645 
    Details
    ISSN: 1573-2657
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mature golden hamster sperm were demembranated with Triton X-100, and the flagellum was reactivated locally by iontophoretic application of ATP at various distances from the base. The response was a brief local straightening of a short length of the flagellum followed by the formation of a pair of bends beyond the two ends of the straight region. The two possible proximo-distal sequences of bends, either PR (principal and reverse bends) or RP, could be distinguished and their incidence studied. The formation of PR and RP bend pairs is interpreted as the result of active sliding of the axonemal doublet subsets 1–4 and 6–9 respectively. The probability of obtaining a PR response increased (1) with the initial local curvature of the resting R bend and (2) with the distance of the stimulated site from the flagellar base; it decreased with the duration of incubation after demembranation. The patterns of response in the middle and the principal piece of the flagellum were basically similar although the former was weaker and more complicated. Quantitative analysis of the ATP-induced movements indicates little or no net microtubule displacement distal to the pair of induced bends, suggesting the cancelling of microtubule displacements in the two bends. However, the expected balance in the rate of growth of the two bends was upset by the decay of one bend simultaneously with decay of the original adjacent bend. Propagation of the interbend region started before the growth of the pair of bends reached its maximum, and seemed to be triggered by a critical bend curvature. Propagation was always in the direction base to tip. Experimental findings also suggest a role in the determination of the waveform for the fibrous structures on the periphery of the axoneme which are characteristic of the mammalian sperm flagellum. The present study strengthens the experimental evidence for the mathematical model which proposes that active sliding occurs mainly in the interbend region and causes bending of segments in opposite directions. In addition our findings indicate that the activation of alternate halves of the axoneme is curvature dependent, suggesting a basis for the flagellar oscillation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00712157
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  • 6
    Electronic Resource
    Electronic Resource
    Immunocytochemical localisation of some lysosomal hydrolases, their presence in luminal fluid and their directional secretion by human epididymal cells in culture (1995)
    Springer
    Cell & tissue research 280 (1995), S. 415-425 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Epididymis ; Efferent ducts ; Cell culture ; Immunocytochemistry ; Immunoprecipitation ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The way in which the human epididymis modifies spermatozoa during their sojourn in this structure might be clarified by knowledge of the nature of its secretions. We have examined the presence of several lysosomal hydrolases in human epididymal tissue and fluids, and their synthesis and secretion by monolayer cultures. Tissues were obtained from men undergoing orchidectomy for prostatic carcinoma. The enzymes cathepsin D and acid α-glucosidase were localised in the lysosomes of epithelial cells from the corpus epididymidis, by an immunocytochemical technique. Cathepsin D was also found in epithelial cells of the efferent ducts within lysosomes, apical vesicles and multivesicular bodies. No immunolocalisation of acid glucosidase in the efferent ducts or on the microvilli of the corpus was demonstrable. Cathepsin D, β-hexosaminidase (N-acetylglucosaminidase) and α-glucosidase were measurable in the luminal fluid from the human corpus epididymidis; β-hexosaminidase was secreted into the culture medium by confluent monolayers of epididymal and efferent duct cells. Immunoprecipitation of cell extracts and culture medium of these cultures incubated with 35S-methionine revealed that the precursors of cathepsin D and β-hexosaminidase were synthesized and secreted by such monolayers. Thus, active lytic enzymes are secreted by the human epididymis and could modify sperm membranes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307815
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  • 7
    Electronic Resource
    Electronic Resource
    Immunocytochemical localisation of some lysosomal hydrolases, their presence in luminal fluid and their directional secretion by human epididymal cells in culture (1995)
    Springer
    Cell & tissue research 280 (1995), S. 415-425 
    Details
    ISSN: 1432-0878
    Keywords: Epididymis ; Efferent ducts ; Cell culture ; Immunocytochemistry ; Immunoprecipitation ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The way in which the human epididymis modifies spermatozoa during their sojourn in this structure might be clarified by knowledge of the nature of its secretions. We have examined the presence of several lysosomal hydrolases in human epididymal tissue and fluids, and their synthesis and secretion by monolayer cultures. Tissues were obtained from men undergoing orchidectomy for prostatic carcinoma. The enzymes cathepsin D and acid α-glucosidase were localised in the lysosomes of epithelial cells from the corpus epididymidis, by an immunocytochemical technique. Cathepsin D was also found in epithelial cells of the efferent ducts within lysosomes, apical vesicles and multivesicular bodies. No immunolocalisation of acid glucosidase in the efferent ducts or on the microvilli of the corpus was demonstrable. Cathepsin D, β-hexosaminidase (N-acetylglucosaminidase) and α-glucosidase were measurable in the luminal fluid from the human corpus epididymidis; β-hexosaminidase was secreted into the culture medium by confluent monolayers of epididymal and efferent duct cells. Immunoprecipitation of cell extracts and culture medium of these cultures incubated with 35S-methionine revealed that the precursors of cathepsin D and β-hexosaminidase were synthesized and secreted by such monolayers. Thus, active lytic enzymes are secreted by the human epididymis and could modify sperm membranes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307815
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  • 8
    Electronic Resource
    Electronic Resource
    Appearance and endocytic activity of epithelial cells from human efferent ducts in primary monolayer culture (1992)
    Springer
    Cell & tissue research 270 (1992), S. 513-519 
    Details
    ISSN: 1432-0878
    Keywords: Reproductive system, male ; Ductuli efferentes ; Epithelial cells ; Cell culture ; Monolayer cultures ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The culture of epithelial cells lining human efferent ducts, obtained from prostatic carcinoma patients, is described. Ciliated cells were observed to beat for at least one month on plastic. On pervious filters low cuboidal cells characterized the monolayers. Cells comprising monolayers over the filter were 5 to 9 μm in height whereas taller cells were found over the original fragments (14 μm). Some non-ciliated cells contained dark and light vacuoles, others were found to lack them. Both non-ciliated and ciliated cells maintained tight junctional complexes restricting the paracellular movement of horseradish peroxidase. Both types of cultured cells exhibited fluid-phase and adsorptive endocytosis from both apical and basal surfaces. It is reported for the first time that the monolayers form high resistance barriers (150 Ω cm2) that prevent the apical medium from draining to the basal compartment over 24 h.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00645053
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  • 9
    Electronic Resource
    Electronic Resource
    Immature rat epididymal epithelial cells grown in static primary monolayer culture on permeable supports (1989)
    Springer
    Cell & tissue research 256 (1989), S. 573-580 
    Details
    ISSN: 1432-0878
    Keywords: Epididymis ; Epithelium ; Monolayer culture ; Histochemistry ; Electron microscopy ; Rat (Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary N-acetylglucosaminidase (NAG), acid phosphatase (ACP) and alkaline phosphatase (AKP) were localised histochemically in fixed cells from the 37-day-old rat epididymis grown in static monolayer culture for 2–8 days. ACP and NAG were cytosolic enzymes found in perinuclear positions, whereas staining of AKP was consistent with a membranous position. These enzymes were also examined in frozen tissue sections of the epididymis, from rats of the equivalent age, where NAG had intense activity in both supra- and infra-nuclear cytoplasm and ACP was more active apically. For the first time AKP was localised along basolateral membranes of the epithelium and in the lumen of the mid-caput region. The monolayer in culture was of principal cells only and they maintained their polarity and ultrastructural characteristics, but the height of the cells was reduced compared to that obtained in situ.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225606
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructure of the perfused rat epididymis: Effect of luminal sodium ion concentration (1982)
    Springer
    Cell & tissue research 226 (1982), S. 407-425 
    Details
    ISSN: 1432-0878
    Keywords: Luminal perfusion ; Rat epididymis ; Epithelial structure ; Absorption ; Water transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The appearance of the rat epididymal epithelium changed when it was perfused in vivo through the lumen with unphysiologically high sodium ion concentrations; dilatation of intercellular spaces (ICS) at threshold concentrations of 30mM-Na+ in the cauda and about 55mM-Na+ in the corpus was associated with absorption of water from the lumen. Despite the distended ICS, junctional complexes appeared intact, and their integrity was confirmed by the exclusion of luminal horseradish peroxidase (HRP) from the ICS, and by demonstrating that circulating [3H]inulin did not enter the lumen. Smooth ER and lipid droplets in the principal cells of the corpus epididymidis were well maintained, and the preservation of granular ER in principal cells of the cauda epididymidis lent morphological support to the continued secretion of protein in this segment. However, occasional distension or involution of inner Golgi cisternae was evident in principal cells after 3–6 h perfusion. In contrast to multivesicular bodies of principal cells, the apical and basal vacuoles characteristic of clear cells changed in size with different perfusing solutions. When low Na+ concentrations were perfused large translucent vacuoles were frequently found in the apical cytoplasm of clear cells in the corpus and cauda epididymidis, and filled vacuoles became larger and showed a decrease in content density in the cauda epididymidis. These large vacuoles were absent from tissue perfused with high Na+ concentrations. Normal pinocytotic activity of both cell types was demonstrated by perfusing HRP which was taken up by the normal route in principal cells, with some transfer to the Golgi cisternae. By far the most HRP was accumulated in clear cell vacuoles irrespective of the composition of the perfusing solution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218369
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