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  • 1
    Electronic Resource
    Electronic Resource
    Effects of long-term daily administration of prostaglandin-E2 on maintaining elevated proximal tibial metaphyseal cancellous bone mass in male rats (1992)
    Springer
    Calcified tissue international 50 (1992), S. 245-252 
    Details
    ISSN: 1432-0827
    Keywords: Prostaglandin E2 ; Long-term treatment ; Cancellous bone ; Bone formation ; Bone resorption ; Bone turnover ; Remodeling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The effects of long-term prostaglandin E2 (PGE2) on cancellous bone in proximal tibial metaphysis were studied in 7-month-old male Sprague-Dawley rats given daily subcutaneous injections of 0, 1, 3, and 6 mg PGE2/kg/day and sacrificed after 60, 120, and 180 days. Histomorphometric analyses were performed on double fluorescent-labeled undecalcified bone specimens. After 60 days of treatment, PGE2 produced diffusely labeled trabecular bone area, increased trabecular bone area, eroded and labeled trabecular perimeter, mineral apposition rate, and bone formation rate at all dose levels when compared with age-matched controls. In rats given PGE2 for longer time periods (120 and 180 days), trabecular bone area, diffusely labeled trabecular bone area, labeled perimeter, mineral apposition, and bone formation rates were sustained at the elevated levels achieved earlier at 60-day treatment. The eroded perimeter continued to increase until 120 days, then plateau. The observation that continuous systemic PGE2 administration to adult male rats elevated metaphyseal cancellous bone mass to 3.5-fold of the control level within 60 days and maintained it for another 120 days indicates that the powerful skeletal anabolic effects of PGE2 can be sustained with continuous administration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296289
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  • 2
    Electronic Resource
    Electronic Resource
    Crystal Structure of Zr2Al3C4 (2005)
    Malden, USA : Blackwell Science Inc
    Journal of the American Ceramic Society 88 (2005), S. 0 
    Details
    ISSN: 1551-2916
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The crystal structure of Zr2Al3C4 was refined by the Rietveld method from conventional X-ray powder diffraction data. The structure was hexagonal (space group P63mc, Z=2) with a=0.334680(6) nm, c=2.22394(3) nm, and V=0.215731(6) nm3, being isomorphous with that of U2Al3C4. The final reliability indices were Rwp=8.57%, Rp=6.06%, and S=1.32. The crystal showed an intergrowth structure with NaCl-type ZrC slabs separated by Al4C3-type Al3C2 layers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1551-2916.2005.00627.x
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  • 3
    Electronic Resource
    Electronic Resource
    Combined deficiency of iron and other divalent cations mitigates the symptoms of iron deficiency in tobacco plants (2003)
    Oxford, UK : Munksgaard International Publishers
    Physiologia plantarum 119 (2003), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: To determine the responses of plants to deficiencies of multiple metals, tobacco plants (Nicotiana tabacum L.) were subjected to treatments that were deficient in combinations of Fe and two other micronutrients, Zn and Mn. The response was measured using macro indices, including plant appearance, FW, chlorophyll concentration, and mineral concentrations, and with a molecular index, the barley (Hordeum vulgare L.) Ids2 promoter/GUS fusion gene system (Yoshihara et al. 2003, Plant Biotech 20: 33–41). Tobacco plants grown in medium with combined deficiencies grew better and had higher chlorophyll concentrations than did plants grown on medium deficient in Fe only, although the measured Fe concentrations in the plant tissues were essentially the same. The Ids2/GUS expression responded to Fe deficiency, but not to Mn or Zn deficiencies in tobacco plants when Fe was present. Tobacco plants grown in medium with combined deficiencies had clearly detectable GUS activity, but the response was significantly lower than that in tobacco plants deficient in Fe only. The Fe-deficiency symptoms were mitigated at both the visible and molecular levels. Although more precise experimental evidence is needed to explain the mitigation mechanism, the balance of minerals was shown to be an important parameter to consider when estimating iron deficiency based on tobacco plant responses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.2003.00126.x
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  • 4
    Electronic Resource
    Electronic Resource
    Is the release of phytosiderophores in zinc-deficient wheat plants a response to impaired iron utilization? (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 92 (1994), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of zinc nutritional status on the time course of phytosiderophore release, and uptake of iron and translocation of iron to the shoot, was studied in nutrient solution cultures for two cultivars of wheat (Triticum aestivum. cv. Aroona: T. durum, cv. Duratit) differing in their susceptibility to zinc deficiency. In the zinc-efficient cultivar Aroona, under zinc deficiency translocation of iron from roots to shoot was significantly decreased in 13- and 15-day-old plants, whereas release of phytosiderophores was enhanced when the plants were 16 days old. As zinc deficiency became more severe in older plains, translocation of iron to the shoot was further decreased and release of phytosiderophores was further enhanced. Resupplying zinc in nutrient solution to zinc-deficient plants significantly increased the translocation of iron to the shoot after 48 and 72 h. Concomitantly the release of phytosiderophores was repressed. The other cultivar Durati classified as zinc-inefficient in field observations differed from cv. Aroona by showing a lower rate of phytosiderophore release under Zinc deficiency, and a less impaired translocation of iron to the shoot. Foliar application of iron citrate to zinc-deficient Aroona plants repressed the release of phytosiderophores and increased iron concentrations in shoot and roots. Application of 55Fe to the leaves demonstrated that retranslocation of iron from the shoot to the roots was not affected by the zinc nutritional status. It is concluded that enhanced release of phytosiderophores in zinc-deficient wheat plants was induced primarily by impaired trans-location of iron lo the shoot.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1994.tb08841.x
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  • 5
    Electronic Resource
    Electronic Resource
    Enhanced tolerance of rice to low iron availability in alkaline soils using barley nicotianamine aminotransferase genes (2001)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 19 (2001), S. 466-469 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] One of the widest ranging abiotic stresses in world agriculture arises from low iron (Fe) availability due to high soil pH, with 30% of arable land too alkaline for optimal crop production. Rice is especially susceptible to low iron supply, whereas other graminaceous crops such as barley are not. A ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/88143
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  • 6
    Electronic Resource
    Electronic Resource
    Thioredoxin h is one of the major proteins in rice phloem sap (1995)
    Springer
    Planta 195 (1995), S. 456-463 
    Details
    ISSN: 1432-2048
    Keywords: Oryza (phloem proteins) ; Phloem sap ; protein ; Sieve tube ; Thioredoxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sieve tubes play important roles in the transfer of nutrients as well as signals. Hundreds of proteins were found in pure phloem sap collected from rice (Oryza sativa L. cv. Kantou) plants through the cut ends of insect stylets. These proteins may be involved in nutrient transfer and signal transduction. To characterize the nature of these proteins, the partial amino-acid sequence of a 13kDa protein, named RPP13-1, that was abundant in the pure phloem sap was determined. A cDNA clone of 687 bp, containing an open reading frame of 122 amino acids, was isolated using corresponding oligonucleotides as a probe. The deduced amino-acid sequence was very similar to that of the ubiquitous thiol redox protein, thioredoxin. The consensus sequences of thioredoxins are highly conserved. No putative signal peptide was identified. Antiserum against wheat thioredoxin h cross-reacted with RPP13-1 in the phloem sap of rice plants. RPP131 produced in Escherichia coli was reactive to antiserum against wheat thioredoxin h. Both E. coli-produced RPP13-1 and the phloem sap proteins catalyzed the reduction of the disulfide bonds of insulin in the presence of dithiothreitol. These results indicate that an active thioredoxin is a major protein translocating in rice sieve tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00202605
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  • 7
    Electronic Resource
    Electronic Resource
    Immunological characterization of a 36 kDa Fe deficiency specific peptide in barley roots (1997)
    Springer
    BioMetals 10 (1997), S. 77-84 
    Details
    ISSN: 1572-8773
    Keywords: barley roots ; graminaceous plants ; immunoblotting ; iron deficiency ; 36 kDa peptide ; 2D–PAGE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In a previous paper we reported that an acidic 36 kDa peptide is the most strongly induced peptide among several peptides induced by Fe deficiency in barley roots. In this paper, polyclonal antibodies were raised against the 36 kDa peptide. This peptide appeared in the roots of all the graminaceous species tested (barley, rye, wheat, oat, maize, sorghum and rice) in response to Fe deficiency. More of the peptide was found in the roots of graminaceous species which secrete higher amounts of mugineic acids (MAs) under Fe deficient nutrition status. Induction of the 36 kDa peptide was first observed on the third day of Fe deficiency, rising to a maximum value on the seventh day. The trend has a positive correlation with secretion of MAs during Fe deficiency. Further, resupply of Fe resulted in a decrease in peptide production on the second day, reaching a control level on the seventh day. The rate of decrease in peptide production was observed to be slower than that of MA secretion. Other nutrient stresses such as B excess, B deficiency, Cu excess, Cu deficiency, Mn excess, Mn deficiency, Zn excess and Zn deficiency induced far less of the peptide. The specific expression of the 36 kDa peptide in roots of graminaceous species under Fe deficiency suggested the positive association of the peptide with a specific Fe deficiency tolerance mechanism in graminaceous plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018323021721
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  • 8
    Electronic Resource
    Electronic Resource
    Mechanism of aluminium tolerance in Cyanidium caldarium (2000)
    Springer
    Hydrobiologia 433 (2000), S. 57-60 
    Details
    ISSN: 1573-5117
    Keywords: aluminium ; Cyanidium caldarium ; acidophile ; thermophile ; red alga
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyanidium caldarium, an acidophilic, thermophilic red alga, specifically tolerates Al. The tolerance increases at lower culture temperatures. The intracellular Al concentration is kept at low levels, especially when the cells are cultured at lower temperatures. Lower Al incorporation accounts for the Al tolerance in this alga. Fe incorporation antagonizes the Al incorporation, implying that Fe transporters incorporate Al ions. Treatment with an uncoupler, carbonylcyanide m-chlorophenylhydrazone, increases the intracellular concentration of Al. These results support the hypothesis that Al ions taken up by the algal cells are exported by an energy-dependent mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004058402425
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  • 9
    Electronic Resource
    Electronic Resource
    The role of potassium in the secretion of mugineic acids family phytosiderophores from iron-deficient barley roots (1999)
    Springer
    Plant and soil 215 (1999), S. 221-227 
    Details
    ISSN: 1573-5036
    Keywords: anion channel ; barley root ; iron deficiency ; LV-SEM-XMA ; mugineic acid family phytosiderophores ; potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Mugineic acid family phytosiderophores (MAs) are secreted from iron-deficient barley roots with high equimolar correlation of potassium. To determine the form of MAs when it is secreted, we investigated the effect of anion channel blockers and valinomycin on the secretion of MAs. Among the anion channel blockers, anthracene-9-carboxylic acid and phenylglyoxal drastically reduced the amount of secreted MAs, while 4,4-diisothiocyano-2,2- stilbene disulfonate slightly inhibited the MAs secretion. Trifluoromethyl-3-phenylamino-2-nicotinic acid reduced the secreted amount to the half of non-treated. This result suggested that MAs are secreted in the form of anion through an anion channel. The elimination of potassium gradient between the cytoplasm and the cell exterior by treatment with valinomycin reduced the amount of secreted MAs. Analysis of potassium distribution in root by LV-SEM-XMA indicated that potassium in the cortex cells of iron-deficient roots is released with MAs secretion and the amount of potassium in the cortex cells decreases after secretion. These results suggested that MAs are secreted in the form of a monovalent anion via anion channels using the potassium gradient between the cytoplasm and the cell exterior.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004546112140
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  • 10
    Electronic Resource
    Electronic Resource
    An iron deficiency-specific cDNA from barley roots having two homologous cysteine-rich MT domains (1991)
    Springer
    Plant molecular biology 17 (1991), S. 531-533 
    Details
    ISSN: 1573-5028
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040651
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