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  • 1
    Publication Date: 2021-05-19
    Description: Red tide event a product of micro algae abundance has elevated in last few years. Loss of aquatic life due to HAB, and its effect on human health, has caused tremendous damage in the world. In this research the effect of (1, 2, 3, 9, and 30) ×10^3 Cells ml^-1 densities of Cochlodinium polykrikoides on survival and histopathological effect on hepatopancreas and gills of Litopenaeus vannamei postlarvae (PLs) and juvenile stages has been investigated. Results showed 100% survival with (1, 2, and 3) ×10^3 Cells ml-1 densities but accumulative mortality in 9×10^3 Cells ml^-1 density were 1.33% and 2.22%, in PLs and juvenile stages respectively, and there is no significant difference between control and treatment groups. accumulative mortality in 3×10^4 Cells ml^-1 density was 4.44% and 17.8% in PLs and juveniles respectively which have significant different with control groups. According to histopathological investigation in this density the infiltration of blood cells in connective tissue of hepatopancreatic tubules and gills lamellae of PLs and juvenile stages were low but high in 3×10^4 Cells ml^-1. The intensity of infiltration in juvenile stages was higher than PLs, which indicate incensement of hepatopancreatic and gills blood cells in higher densities of this phytoplankton. The end result of the study indicates that in high densities of C. polykrikoides survival rate in PLs and juveniles decreased and according to histopathological study the most important possible factor in shrimp mortality when exposure to C. polykrikoides was settlement of phytoplankton on gills lamellae and preventing oxygen exchange of shrimp.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Cochlodinium polykrikoides ; Litopenaeus vannamei ; Bloom ; Histopathological ; Survival ; Investigation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 78pp.
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  • 2
    Publication Date: 2021-05-19
    Description: White Spot Disease (WSD) is a important disease due to economic impacts in shrimp industries. Spreading of this disease in shrimp farms can caused a 100% mortality during 3-10 days. Therefore control of this disease is a strategy in shrimp industry. Vaccination is a way to control of WSD. In several years ago during a project several type vaccine of this virus by association of Atomic Energy Organization is produced that among of them the virus inactivated by GAMA radiation had better results in laboratory. To test of this vaccine in field, a research pilot was carried out. Initially 20000 shrimp napliies were obtained from one of commercial hatchery in Bushehr province, then devided to two groups vaccinated and unvaccinated. The vaccinated group also devided . 26and PL12 , group B vaccinated at PL15and PL 5to two groups, group A that vaccinated at postlarva e (PL) Rrsults showed that difference of growth performance and survival rate between vaccinated and unvaccinated without challenge with WSV after 80 days is not significantly (P〈0.05). but survival rate in vaccinated groups after challenge with WSV was significantly (P〈0.05) further the unvaccinated group. Also the results showed survival rate of group B after challenge with WSV is better than group A and this difference was significantly (P〈0.05). this study showed vaccination of shrimp postlarvae with GAMA radiation vaccine can control of shrimp mortality in incidence of WSD in farms.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; WSSV ; Gamma radiation ; Vaccination ; Y-ray ; White spot disease ; Shrimp ; Nauplii ; Growth ; Survival rate ; Mortality
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 65pp.
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  • 3
    Publication Date: 2021-05-19
    Description: After the outbreak of various viral diseases, specifically white spot syndrome (WSS) in farmed Litopenaeus vannamei in the past few years, this non-native shrimp species in Iran requires a generator-builder with specific a pathogen-free approach. This Research was done by the Iran Shrimp Research Center (ISRC) to access accurate information regarding specific pathogen-free shrimp production and avoid using shrimp broodstock production through foreign investors. This research was part of the molecular viral study. For this trial, selected shrimps in farms were sampled and screened for main viruses (OIE list). Shrimps without infection were brought to the quarantine system for one month and at the end of the quarantine they were screened again. In addition, in winter spending, before and after spawning, it’s offspring in F1 and F2 were also screened for viral pathogens by IQ2000 kit at the Iran Shrimp Research Center lab and Iranian veterinary organization lab. All shrimp products such as fresh and consumed food were tested for OIE list at all stages during project performance and the result of tests were negative. Sequenced and molecular tests with specific primers were used to determine the presence of infected samples carrying the virus in any of the products, such as shrimp and consumed food, the infected samples. The result of all the tests during last three years, shows that all the shrimp and their food products were pathogen-free and safe to use.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrim ; SPF ; Virus ; White Spot Syndrome Virus ; WSS ; Spawning ; Molecular
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 48pp.
    Location Call Number Expected Availability
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  • 4
    Publication Date: 2021-05-19
    Description: DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. in this research, samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: DNA barcode ; Shrimp ; Litopenaeus vannamei ; Penaeus semisulcatus ; Fenneropenaeus merguiensis ; Metapenaeus affinis ; Parapenaeopsis stylifera ; Fenneropenaeus indicus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 72pp.
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  • 5
    Publication Date: 2021-05-19
    Description: In the present Shrimp industry Has become a huge industry . The shrimp culture in Bushehr province began with Penaeus semisulcatus and Fenneropenaeus indicus and with introducing Litopenaeus vannamei in Iran Was known as a culture species.The shrimp production rate in IRAN is about 23000 metric ton. Outbreak of Various diseases and Biosecurity are most important things to produce Specific Patogen Free(SPF) shrimp.In this regard, production of SPF shrimp is The main requirements of shrimp industry.The survey on growth and srvival larve of different generation of Litopenaeus vannamei project was carried out in 2013 and 2014.Broodstock selecting ,propagation, culture and shrimp generation Was performed in 2012 until 2014.In 2014 we produced 4300 pieces of prebroodstock with 22.38 gram and 86 percent survival rate after 120 days of culture.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannmei ; Specific Patogen Free ; SPF ; Cultue ; Shrimp culture ; Penaeus semisulcatus ; Fenneropenaeus indicus ; Survey ; Growth ; Survival ; Larvae
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 40pp.
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  • 6
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25661 | 18721 | 2018-10-14 03:01:48 | 25661 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Red tide event a product of micro algae abundance has elevated in last few years. Loss of aquatic life due to HAB, and its effect on human health, has caused tremendous damage in the world. In this research the effect of (1, 2, 3, 9, and 30) ×10^3 Cells ml^-1 densities of Cochlodinium polykrikoides on survival and histopathological effect on hepatopancreas and gills of Litopenaeus vannamei postlarvae (PLs) and juvenile stages has been investigated. Results showed 100% survival with (1, 2, and 3) ×10^3 Cells ml-1 densities but accumulative mortality in 9×10^3 Cells ml^-1 density were 1.33% and 2.22%, in PLs and juvenile stages respectively, and there is no significant difference between control and treatment groups. accumulative mortality in 3×10^4 Cells ml^-1 density was 4.44% and 17.8% in PLs and juveniles respectively which have significant different with control groups. According to histopathological investigation in this density the infiltration of blood cells in connective tissue of hepatopancreatic tubules and gills lamellae of PLs and juvenile stages were low but high in 3×10^4 Cells ml^-1. The intensity of infiltration in juvenile stages was higher than PLs, which indicate incensement of hepatopancreatic and gills blood cells in higher densities of this phytoplankton. The end result of the study indicates that in high densities of C. polykrikoides survival rate in PLs and juveniles decreased and according to histopathological study the most important possible factor in shrimp mortality when exposure to C. polykrikoides was settlement of phytoplankton on gills lamellae and preventing oxygen exchange of shrimp.
    Keywords: Ecology ; Iran ; Cochlodinium polykrikoides ; Litopenaeus vannamei ; Bloom ; Histopathological ; Survival ; Investigation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 78
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  • 7
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25738 | 18721 | 2018-10-12 17:49:02 | 25738 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Shrimp aquaculture as one of the most important activities in the world and Iran is expanding. Bacteria and fungi of the most common infectious agents causing diseases are in the hatchery and shrimp. The most important bacteria causing diseases in the hatcheries and shrimp Farms, bacteria are Vibrionaceae family. The fungi can be identified as Fusarium, Mucor, Cladosporium, Aspergillus, Penicillium and yeast. Utilized of water supply system in this project for breeding and reproduction of shrimp is a controlled system. The purpose of this action, control and stabilization of water quality during the breeding period and in all sectors. This system act as a part of biological security. Incoming water before the utilization was monitored for the total bacteria count, fungi and as well as Vibrionaceae family, each 15 days. If contamination is too predictable, additional tests and necessary treatments were carried out to improve the quality of the water. The water, after use in the system and after passing through the filtration system, was guided out.
    Keywords: Health ; Iran ; Specific pathogen free ; SPF ; Bacterial agents ; Fungal agents ; Water coality in shrimp hatchryies and culture system ; Fusarium ; Mucor ; Cladosporium ; Aspergillus ; Penicillium ; Vibrionaceae
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 54
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  • 8
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25749 | 18721 | 2018-10-13 07:31:35 | 25749 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: After the outbreak of various viral diseases, specifically white spot syndrome (WSS) in farmed Litopenaeus vannamei in the past few years, this non-native shrimp species in Iran requires a generator-builder with specific a pathogen-free approach. This Research was done by the Iran Shrimp Research Center (ISRC) to access accurate information regarding specific pathogen-free shrimp production and avoid using shrimp broodstock production through foreign investors. This research was part of the molecular viral study. For this trial, selected shrimps in farms were sampled and screened for main viruses (OIE list). Shrimps without infection were brought to the quarantine system for one month and at the end of the quarantine they were screened again. In addition, in winter spending, before and after spawning, it’s offspring in F1 and F2 were also screened for viral pathogens by IQ2000 kit at the Iran Shrimp Research Center lab and Iranian veterinary organization lab. All shrimp products such as fresh and consumed food were tested for OIE list at all stages during project performance and the result of tests were negative. Sequenced and molecular tests with specific primers were used to determine the presence of infected samples carrying the virus in any of the products, such as shrimp and consumed food, the infected samples. The result of all the tests during last three years, shows that all the shrimp and their food products were pathogen-free and safe to use.
    Keywords: Biology ; Iran ; Shrim ; SPF ; Virus ; White Spot Syndrome Virus ; WSS ; Spawning ; Molecular
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 48
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  • 9
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25750 | 18721 | 2018-10-13 07:33:30 | 25750 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. in this research, samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species.
    Keywords: Biology ; Information Management ; Iran ; DNA barcode ; Shrimp ; Litopenaeus vannamei ; Penaeus semisulcatus ; Fenneropenaeus merguiensis ; Metapenaeus affinis ; Parapenaeopsis stylifera ; Fenneropenaeus indicus
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 72
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  • 10
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25546 | 18721 | 2018-10-05 16:39:40 | 25546 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: White Spot Disease (WSD) is a important disease due to economic impacts in shrimp industries. Spreading of this disease in shrimp farms can caused a 100% mortality during 3-10 days. Therefore control of this disease is a strategy in shrimp industry. Vaccination is a way to control of WSD. In several years ago during a project several type vaccine of this virus by association of Atomic Energy Organization is produced that among of them the virus inactivated by GAMA radiation had better results in laboratory. To test of this vaccine in field, a research pilot was carried out. Initially 20000 shrimp napliies were obtained from one of commercial hatchery in Bushehr province, then devided to two groups vaccinated and unvaccinated. The vaccinated group also devided . 26and PL12 , group B vaccinated at PL15and PL 5to two groups, group A that vaccinated at postlarva e (PL) Rrsults showed that difference of growth performance and survival rate between vaccinated and unvaccinated without challenge with WSV after 80 days is not significantly (P〈0.05). but survival rate in vaccinated groups after challenge with WSV was significantly (P〈0.05) further the unvaccinated group. Also the results showed survival rate of group B after challenge with WSV is better than group A and this difference was significantly (P〈0.05). this study showed vaccination of shrimp postlarvae with GAMA radiation vaccine can control of shrimp mortality in incidence of WSD in farms.
    Keywords: Health ; Iran ; Litopenaeus vannamei ; WSSV ; Gamma radiation ; Vaccination ; Y-ray ; White spot disease ; Shrimp ; Nauplii ; Growth ; Survival rate ; Mortality
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 65
    Location Call Number Expected Availability
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