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  • Chromosome mosaicism  (2)
  • 1
    Digitale Medien
    Digitale Medien
    In vitro culture ofBellevalia romana (L.) Rchb. (1986)
    Springer
    Protoplasma 132 (1986), S. 58-63 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Bellevalia romana ; Callus ; Nuclear DNA content ; Chromosome mosaicism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Bellevalia romana (L.) Rchb., a monocotyledonous plant characterized by few (2 n=2 x=8) and very large chromosomes, is a useful subject for studying developmental problemsin vitro. Cytological analysis of callus revealed that the majority of cells were diploid, but the remaining cells had aneuploid nuclei with a wide range of chromosome numbers, tetraploid and haploid nuclei. The frequency of aneuploid and polyploid cells was higher in callus grown in the presence of 2,4-D than in callus grown in NAA plus BAP. These nuclei seemed to increase with the duration of culture. The chromosome number distribution as determined by chromosome counts in calli at different culture times was confirmed by DNA cytophotometry. Chromosome number mosaicism (mixoploidy and aneusomaty) also occurred in all root apices of 9 out of 46 plantlets regenerated from callusvia adventitious shoots.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01275790
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 2
    Digitale Medien
    Digitale Medien
    Nuclear cytology of callus and plantlets regenerated from pea (Pisum sativum L.) meristems (1987)
    Springer
    Protoplasma 141 (1987), S. 121-125 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Callus ; Chromosome mosaicism ; Meristem culture ; Pisum sativum ; Plant regeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The chromosomal status of calli and plantlets regenerated fromPisum sativum shoot apical meristems was studied. Chromosome mosaicism (aneusomaty) occurs during callus induction and proliferation, mostly owing to nuclear fragmentation prior to mitosis in the first days of culture. Plantlets regenerated from calli are diploid or aneusomatic, but a selective advantage of diploid cells (diplontic selection) takes place with plantlet growth. The results are discussed in relation to the possibility of inducing chromosomal and/or genetic variability by using meristematic tissues as expiants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01272893
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
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