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  • Gene regulation  (1)
  • Multigene family  (1)
  • Recombination  (1)
  • 1990-1994  (2)
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  • 1990-1994  (2)
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  • 1
    Digitale Medien
    Digitale Medien
    Structure and regulation of the isocitrate lyase gene ICL1 from the yeast Saccharomyces cerevisiae (1993)
    Springer
    Current genetics 23 (1993), S. 375-381 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Saccharomyces cerevisiae ; Isocitrate lyase ; Gene regulation ; Ethanol induction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The ICL1 gene encoding the isocitrate lyase from Saccharomyces cerevisiae was cloned and sequenced. A reading frame of 557 amino acids showing significant similarity to isocitrate lyases from seven other species could be identified. Construction of icl1 null mutants led to growth defects on C2 carbon sources while utilization of sugars or C3 substrates remained unaffected. Using an ICL1-lacZ fusion integrated at the ICL1 locus, a more than 200-fold induction of β-galactosidase activity was observed after growth on ethanol when compared with glucose-repressed conditions. A preliminary analysis of the ICL1 upstream region identified a 364-bp fragment necessary and sufficient for this regulatory phenotype. Sequence motifs also present in the upstream regions of co-regulated genes were found within this region.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00312621
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Genetical and RFLP studies at the Mla locus conferring powdery mildew resistance in barley (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 713-718 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Barley ; Multigene family ; Mla locus ; Recombination ; RFLP marker
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The complex structure of the multigene family at the Mla locus conferring powdery mildew resistance in barley was studied by making diallel crosses between several near-isogenic lines carrying different Mla alleles. The mode of inheritance of the Mla alleles investigated was determined to be dominant for Mla1, Mla6, Mla7 and Mla13 and semidominant for Mla3, Mla12 and Mla20. F1 plants were backcrossed to the susceptible recurrent parent in order to identify susceptible and double-resistant recombinants in the BC1F1 generation. Out of 17605 progenies tested in the BC1F1 generation, two susceptible recombinants, one between Mla1 and Mla12 and one between Mla13 and Mla20 were confirmed. The former was also verified by RFLP analysis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00225010
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
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