ISSN:
1612-1112
Schlagwort(e):
Column liquid chromatography
;
Electrothermal atomic absorption spectrometry
;
Vitamin B12 analogues
;
Cobalamins and cobalt
;
Meat
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Summary The coupling of liquid chromatography and electrothermal atomic absorption spectrometry (LC-ETAAS) lowers the detection limit for identification of vitamin B12 analogues. Cobalamins and aqueous cobalt (II) were separated by reversed-phase liquid chromatography using a linear gradient: 26∶74 (v/v) methanol:0.05 M phosphate buffer (pH 4.2) to 50∶50 mixture over 8 min. The vitamins were quantitatively determined in the column effluent by measuring total cobalt by ETAAS. The analysis of meat and liver extracts by LC-ETAAS showed that the matrix did not interfere with the determination of cobalt. Hence, recoveries of cobalt in spiked meat and liver samples were satisfactory.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02290293
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