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  • Articles  (103)
  • Springer  (73)
  • Elsevier  (30)
  • 1980-1984  (70)
  • 1970-1974  (33)
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  • Articles  (103)
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  • 1
    Electronic Resource
    Electronic Resource
    Preparation of a human lung purified plasma membrane fraction: Confirmation by enzyme markers, electron microscopy, and histamine H1 receptor binding (1984)
    Springer
    The journal of membrane biology 79 (1984), S. 33-39 
    Details
    ISSN: 1432-1424
    Keywords: plasma membrane ; lung ; histamine receptors ; 5′ nucleotidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary A simple and rapid method of isolating plasma membranes from human peripheral lung tissue is described. The method involves homogenization of tissue in 0.25m sucrose-buffered medium followed by differential and sucrose density gradient centrifugation. Enzymatic and morphological characterization of the plasma membrane fraction revealed minimal contamination by nonplasma membrane fragments. The isolated plasma membranes showed an 18-fold purification of 5′-nucleotidase activity compared to the original homogenate. Electronmicroscopic studies of the plasma membrane fraction revealed the presence of small membrane vesicles having a trilaminar membrane structure. To further examine the purity of the plasma membrane preparation, the binding of the H1 receptor antagonist,3H pyrilamine, to the plasma membrane-enriched fraction was compared to the binding to crude membrane preparations. Both the plasma membrane-enriched fraction and the crude membrane preparation had similar Kd's for the histamine antagonist, but the plasma membrane-enriched fraction had a threefold greater binding capacity, reflecting the relative enrichment of plasma membranes of the preparation. Thus, a method has been developed for the isolation of plasma membranes from human peripheral lung which should provide material for a variety of biochemical and pharmacological studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868524
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  • 2
    Electronic Resource
    Electronic Resource
    Discrimination between the red- and far-red-absorbing forms of phytochrome from Avena sativa L. by monoclonal antibodies (1984)
    Springer
    Planta 160 (1984), S. 382-384 
    Details
    ISSN: 1432-2048
    Keywords: Avena (phytochrome) ; Immunological discrimination ; Monoclonal antibody ; Phytochrome (red-, far-red absorbing forms)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A set of rat monoclonal antibodies (ARC MAC 48 to 52 and 54 to 56), raised to phytochrome from dark-grown seedlings of Avena sativa L. was tested for the ability to discriminate between the red-absorbing (Pr) and far-red-absorbing (Pfr) forms of phytochrome by indirect enzyme-linked immunosorbent assay. MAC 50 bound more strongly to Pfr and MAC 49 and 52 showed preferential binding to Pr from extracts of dark-grown Avena seedlings; MAC 50 also bound more strongly to Pfr from brushite-purified phytochrome. The remainder of the monoclonal antibodies and a rabbit polyclonal antiphytochrome preparation did not discriminate between Pr and Pfr. The results provide evidence for conformational changes in defined regions of the phytochrome apoprotein upon photoconversion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393420
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  • 3
    Electronic Resource
    Electronic Resource
    Regulation of swelling of etiolated-wheat-leaf protoplasts by phytochrome and gibberellic acid (1983)
    Springer
    Planta 158 (1983), S. 416-421 
    Details
    ISSN: 1432-2048
    Keywords: Gibberellin and protoplast swelling ; Phytochrome ; Protoplast (swelling) ; Triticum (protoplast swelling)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of light on the size of intact protoplasts isolated from the primary leaves of etiolated Triticum aestivum was studied. A 2-min red-light irradiation in the presence of 1 mM KCl was sufficient to cause a swelling of protoplasts compared with those maintained in darkness. The effect was photoreversible by far-red light over two light cycles, indicating the involvement of phytochrome. At 4°C, escape from reversibility occurred between 2 and 5 min after the exposure to red light. In exposure-response experiments, 20 s red light at 27 μmol m-2s-1 was sufficient to saturate the response. Exogenous gibberellic acid added in darkness in the presence of KCl also induced protoplast swelling. Gibberellins may act as an intermediate in the phytochrome-induced swelling of protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397734
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  • 4
    Electronic Resource
    Electronic Resource
    Calmodulin-like activity in a mineralising tissue: The rat molar tooth germ (1981)
    Springer
    Calcified tissue international 33 (1981), S. 545-548 
    Details
    ISSN: 1432-0827
    Keywords: calmodulin ; calcium ; mineralisation ; tooth germ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Calmodulin, a calcium binding protein, has been implicated in the regulation of many calcium-dependent biological processes. Since calcium has an important role in hard tissue genesis, both at intra- and extracellular levels, we anticipate that calcium binding proteins may modulate this process. The present study investigated a mineralising tissue, the rat molar tooth germ, to determine the presence of calmodulin-like activity. A heat-treated cell-free extract of tooth germs provided enhancement of Ca2+-dependent Mg2+-ATPase and 3′:5′-nucleotide phosphodiesterase activity. No enhancement occurred in the absence of calcium or in the presence of trifluoperazine. SDS-polyacrylamide gel electrophoresis of this extract revealed a protein band of approximately 18,000 mol. wt. These findings indicate the presence of calmodulin-like activity in rat molar tooth germs and support the proposal that calcium and calcium binding proteins, in particular calmodulin, have a major regulatory role in the biology of mineralising tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02409487
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  • 5
    Electronic Resource
    Electronic Resource
    The gregarisation pheromone of locusts (1970)
    Springer
    Chromosoma 29 (1970), S. 462-473 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Swarming locusts show three physical criteria, i.e. the phase changes of melanisation of the nymphal stages or hoppers, of the proportions of certain body parts (morphometric ratios), and increased genetic recombination (meiotic chiasma frequencies) in the adult. The control of these changes, initiated by aggregation into swarms, i.e. gregarisation, seems to be vested in a pheromone which is produced by all hoppers in both the solitaria and gregaria phases, also by hoppers of the albino strain. Such a pheromone can be extracted from the locust room air and from the locust, these extracts showing high activity in bioassays, primarily in increased chiasma frequencies but also in hopper colour. The extract in risella oil is more efficient than that in petroleum ether and can be distilled to yield an active distillate. The pheromone is secreted in the faeces of hoppers but not of adults. There is evidence in faeces bioassays that all three physical criteria are affected; the pheromone may be called locustone. It is manufactured or secreted in a specific section of the alimentary canal, i.e. the crop. Reception is not through the antennae but through the stigmata. Preliminary chemical analysis of a risella oil air extract distilled into various other solvents showed the presence of a relatively simple saturated aliphatic chain with a carbonyl function, perhaps a ketone or an ester.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00281928
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  • 6
    Electronic Resource
    Electronic Resource
    Chronic exposure of goldfish-derived cell cultures to toxaphene alters the replication of Goldfish Virus-2 (1982)
    Springer
    Bulletin of environmental contamination and toxicology 29 (1982), S. 731-733 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01606114
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  • 7
    Electronic Resource
    Electronic Resource
    Toxicity and intracellular localization of carbaryl and 1-naphthol in cell cultures derived from goldfish (1983)
    Springer
    Bulletin of environmental contamination and toxicology 30 (1983), S. 99-104 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01610105
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  • 8
    Electronic Resource
    Electronic Resource
    Toxicity of certain insecticides to protozoa (1982)
    Springer
    Bulletin of environmental contamination and toxicology 28 (1982), S. 628-631 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01605596
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  • 9
    Electronic Resource
    Electronic Resource
    An overview of sedimentation on the amazon continental shelf (1984)
    Springer
    Geo-marine letters 4 (1984), S. 207-210 
    Details
    ISSN: 1432-1157
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Sedimentological, geochemical, and physical-oceanographic studies of the Brazilian continental shelf near the Amazon River help provide a broad understanding of this major sediment dispersal system. Amazon River sediment accumulates as a subaqueous delta, with the most rapid accumulation (∼10 cm/yr) occurring near the seaward edge of the topset beds and in the foreset beds. Amazon River sediment is dispersed northwestward along the shelf and is transported beyond the Brazilian border. Radiographic studies of sediment cores delineate three sedimentary environments: interbedded mud and sand, faintly laminated mud, and bioturbated mud. The distribution of these environments is a function of proximity to the river mouth and of sediment accumulation rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02281707
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  • 10
    Electronic Resource
    Electronic Resource
    Isolation of paraquat-tolerant mutants from tomato cell cultures (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 169-176 
    Details
    ISSN: 1432-2242
    Keywords: Tomato ; Cell cultures ; Herbicidetolerant mutant ; Methyl viologen ; Paraquat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tomato callus clones selected for the ability to grow at paraquat concentrations lethal to wild-type cells were found at an approximate frequency of 5×10−8 per viable cell. Diploid plants were regenerated from nine of the nineteen paraquat-tolerant callus clones isolated. Although some of these plants appeared normal, others had altered morphology and reduced vigor and fertility. New callus cultures initiated from these regenerated plants typically had at least a 30-fold increase over the wild type in tolerance to paraquat. Tests on callus from sexual progeny showed that the paraquat-tolerant phenotypes of clones PQT4, PQT6, and probably also PQT13 resulted from dominant nuclear mutations, but the number of loci involved is not yet known. Paraquat spray experiments indicated that slight paraquat-tolerance was expressed at the plant level in PQT13, but not in any of the other clones tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303704
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