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  • 1
    Digitale Medien
    Digitale Medien
    Some aspects of ovular development during megasporogenesis inPisum sativum L. (1980)
    Springer
    Cellular and molecular life sciences 36 (1980), S. 657-658 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary This work reports some findings which will aid us in our understanding of the female phase ofPisum sativum embryology. We have observed that the central planes between one ovule and the next form an angle of 60°. We suggest that this ovule arrangement may make it easier for the pollen tube to reach the ovular micropyle in the fertilization process. On the other hand, ovary and ovule growth do not depend on the megasporogenesis stage but have individual characteristics for each plant, every one depending on its own phenotype.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01970121
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Observations of a certain arrangement in the nucleolar chromatin after continuous ethidium bromide treatment (1977)
    Springer
    Cellular and molecular life sciences 33 (1977), S. 1033-1035 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary This work is an ultrastructural and cytochemical study of a structure observed in the nucleolus of Allium cepa meristematic cells after nucleolar disgregation, by a continuous treatment of 12 h with ethidium bromide. The ultrastructural and cytochemical data allow us to consider this structure as the intranucleolar chromatin collapsed by the effect of the drug.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01945952
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Nuclear membrane and chromatin network (1965)
    Springer
    Cellular and molecular life sciences 21 (1965), S. 627-628 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Résumé Il existen un certain rapport entre la chromatine et des petites invaginations de la membrane nucléaire. Nous estimons que la charge positive de la membrane et la charge négative des chromosomes peut être la cause inductrice.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02144044
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Development of the middle lamella in rib meristem cells (1968)
    Springer
    Cellular and molecular life sciences 24 (1968), S. 514-516 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Resumen La lámina media que separa transversalmente a las células del meristemo radical en columna se desarrolla, en virtud del aporte de pequeñas vesículas del aparato de Golgi, incrementando su espesor desde 0,1–0,2 hasta 0,4–0,5 µ. Las características morfológicas de estas paredes transversales permiten distinguir fácilmente las células hermanas y hasta las 4 células que se originan de 1 célula madre en 2 ciclos de división.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02144426
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Role of Golgi vesicles in plant cell elongation (1968)
    Springer
    Cellular and molecular life sciences 24 (1968), S. 926-926 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Resumen La rápida elongación de las células durante la diferenciación se debe, entre otros motivos, a un incremento de la membrana envolvente; este incremento es originado por el aporte de vesículas del aparato de Golgi cuyas membranas coalecen con el plasmalema y su contenido se deposita externamente junto al material amorfo de la membrana.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02138659
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    A simple staining technique for electron microscopy with lead-uranyl acetate (1967)
    Springer
    Cellular and molecular life sciences 23 (1967), S. 316-317 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Resumen Mediante el empleo de una solución saturada de acetato de uranilo-plomo en acetona de 70%, durante el proceso convencional de deshidratación, se ha conseguido incrementar marcadamente el contraste electrónico en ápices radicales dePhalaris canariensis, sin riesgo alguno de contaminación o deformación de las estructuras.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02135708
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Evolution of the cytoplasmic organelles during female meiosis in Pisum sativum L. (1981)
    Springer
    Planta 151 (1981), S. 215-225 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Metosis (organelles) ; Organelles (during meiosis)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In this paper we have traced the evolution of the cytoplasmic organelles in the female germinal cell of Pisum sativum L., from the beginning of meiosis to the early stages of the maturing megaspore, in order to correlate the morphological changes with the physiological aspects of megasporogenesis. A process of intense cytoplasmic vacuolation takes place in the megaspore mother cell (MMC) during prophase I, probably proceeding from the smooth endoplasmic reticulum and dictyosomes; it results in the formation of big vacuoles, which play a role in MMC polarization. By means of this polarization most plastids and mitochondria are incorporated into the functional megaspore at the end of meiosis. There are plastid and mitochondria cycles which consist of dedifferentiation followed by redifferentiation, During these cycles a transient morphology appears, called a cup-shaped form, which we interpret as an expression of low organelle activity. The wall of the MMC thickens throughout megasporogenesis and loses its plasmodesmata during middle prophase I. The ribosome population is reduced during prophase I and then restored during the early stages of the megaspore maturing process, as shown by the quantitative study that we have carried out. The nucleolar cytoplasmic bodies play a part in this restoring process. These bodies have a special morphology and appear to be originated from the activity of the nucleolar organizing region (NOR) during nucleolar disorganization in prophase I. We think that this cytoplasmic evolution is a response to nuclear genic recombination, in order to provide the most adequate expression of the zygote genome.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00395172
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Distribution of B-36 nucleolar protein in relation to transcriptional activity in plant cells (1992)
    Springer
    Chromosoma 102 (1992), S. 41-49 
    Details
    ISSN: 1432-0886
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The Mr 34000 nucleolar protein B-36 was immunolocalized using a mouse monoclonal antibody in different plant cells by light and electron microscopy. Specific immunofluorescence was observed in nucleoli of cryofixed and cryosectioned material. Immunoelectron microscopy was performed on ultrathin cryosections and Lowicryl sections. Immunogold labelling revealed that B-36 is mainly localized in the nucleolar dense fibrillar component (DFC). The fibrillar centres and the granular component were practically devoid of gold particles. Nucleoli with different rates of transcriptional activity and, as a consequence, with different morphologies showed the same immunolocalization. This was true for both somatic and germinal tissues of the different plant species studied: Allium cepa root meristematic cells and Scilla peruviana and Capsicum annuum pollen grains. The presence of the B-36 antigen in the DFC of nucleoli with very different rates of transcriptional activity indicates that it is a constitutive protein. Moreover, the localization suggests that it could play a role in the transcription and/or early processing of rRNA. The presence of B-36 in the different plant tissues and species studied, confirms once more its high level of conservation throughout evolution. Another Mr 34000 protein called fibrillarin was also found in the DFC. Taking these and other published data together, we think that the antibodies used in the different studies are probably binding different epitopes of identical or very similar Mr 34000 nucleolar proteins.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00352289
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Transmission electron microscopic studies on the mitotic cycle of nucleolar proteins impregnated with silver (1982)
    Springer
    Chromosoma 85 (1982), S. 261-273 
    Details
    ISSN: 1432-0886
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Hela cells were impregnated with silver according to Paweletz et al. (1967). In cells in mitosis not only the nucleolar organizer regions (NORs) are strongly impregnated but also part of the nucleolar material, which accumulates in and around the chromosomes. The treatment with adenosine, which in interphase cells spreads the nucleolar material within the nucleus, also distributes the argentophilic material in and around the chromosomes. During the reconstruction phase this material reassembles around the NORs to form parts of the new nucleolus. The silver impregnation technique clearly demonstrates that two main components are responsible for the argentophily of the nucleolus. This is in agreement with the results obtained by Lischwe et al. (1979).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00294970
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    A study on nucleolar silver staining in plant cells. The role of argyrophilic proteins in nucleolar physiology (1983)
    Springer
    Chromosoma 88 (1983), S. 149-155 
    Details
    ISSN: 1432-0886
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Using a high resolution nucleolar silver staining technique, we have studied the distribution of silver grains at interphase and mitosis. The nucleolar fibrillar centers and the fibrillar component, as well as the mitotic nucleolar organizing region (NOR), are argyrophilic. Of the NOR structures, the fibrillar component is transcriptionally active, but the fibrillar centers and the mitotic NORs are not. Treatment with cycloheximide (CHM), which indirectly impairs rRNA synthesis, does not affect the pattern of silver staining. The currently assumed correlation between activity and Ag-NOR staining appears questionable. On the other hand, all Ag-NOR-stained structures contain decondensed chromatin, whereas condensed portions of this chromatin are not stained by silver. In CHM-treated cells there is apparently no chromatin condensation accompanying inhibition of rRNA synthesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00327336
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