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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 44 (2002), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Spo0A~P is the essential response regulator and transcription factor for sporulation initiation in Bacillus subtilis. The phosphorylation level of Spo0A in the cell is determined by the sensor kinase activity of the phosphorelay, donating phosphoryl groups, and the antagonistic effects of dephosphorylation mediated by the Rap and Spo0E families of phosphatases. In this study, spo0A mutations were generated that encoded proteins less sensitive to the activity of Spo0E than the wild-type protein. The Spo0A substitutions N12K, P60S, L62P and F88L are surface exposed and localize to the same face of the molecule as the active site and in its close proximity on the β1–α1, β3–α3 and β4–α4 loops. The corresponding surface in the Spo0F response regulator was shown previously to be involved in the interaction with the RapB phosphatase, as well as the KinA histidine kinase and the Spo0B phosphotransferase. Thus, residues occupying the same position (N12:Q12, F88:Y84) and the same loops in Spo0A or Spo0F are involved in the interaction with the structurally unrelated Spo0E and RapB phosphatases, respectively, in addition to kinases and phosphotransferase. The specificity in phosphatase target recognition must be the result of side-chain variability within the response regulators and the interactions they promote. The residues involved in Spo0E interaction are identical in all Spo0A orthologues from spore-forming Bacilli encoding Spo0E phosphatases.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Weed research 44 (2004), S. 0 
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Classical Mendelian experiments were conducted to determine the genetics and inheritance of quinclorac and acetolactate synthase (ALS)-inhibitor resistance in a biotype of Galium spurium. Plants were screened with the formulated product of either quinclorac or the ALS-inhibitor, thifensulfuron, at the field dose of 125 or 6 g active ingredient (a.i.) ha−1 respectively. Segregation in the F2 generation indicated that quinclorac resistance was a single, recessive nuclear trait, based on a 1 : 3 segregation ratio [resistant : susceptible (R : S)]. Resistance to ALS inhibitors was due to a single, dominant nuclear trait, segregating in the F2 generation in a 3 : 1 ratio (R : S). The genetic models were confirmed by herbicide screens of F1 and backcrosses between the F1 and the S parent. F2 plants that survived quinclorac treatment set seed and the resulting F3 progeny were screened with either herbicide. Quinclorac-treated F3 plants segregated in a 1 : 0 ratio (R : S), hence F2 progenitors were homozygous for quinclorac resistance. In contrast, F3 progeny segregated into three ratios: 1 : 0, 3 : 1 and 0 : 1 (R : S) in response to ALS-inhibitor treatment. This segregation pattern indicates that their F2 parents were either homozygous or heterozygous for ALS-inhibitor resistance. Therefore, there were clearly two distinct resistance mechanisms encoded by two genes that were not tightly linked as demonstrated by segregation patterns of the F3.
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  • 3
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effects of mycorrhizal infection, soil P availability and fruit production on the male function of reproduction were examined in two cultivars of tomato (Lycopersicon esculentum Mill.). Tomato plants were grown in a greenhouse under three treatment combinations: non-mycorrhizal, low P (NMPO); non-mycorrhizal, high P (NMP3); and mycorrhizal, low P (MPO). In addition, all treatment combinations were grown both with and without fruit. Fruit production decreased final leaf biomass, flower production and in vitro pollen tube growth rates, often reducing the beneficial effects of increased P uptake. Thus, fruit production diverted resources from subsequent vegetative growth, flower production and pollen development. As the growing season progressed, mean pollen production per flower and in vitro germination and tube growth decreased. Mycorrhizal infection and high soil P conditions increased final leaf biomass, flower production, mean pollen production per flower (in one cultivar) and in vitro pollen tube growth rates. Thus, mycorrhizal infection and high soil P conditions increased pollen quantity and quality, thereby enhancing fitness through the male function. Similar trends in these treatments suggested that mycorrhizal effects on the male function were largely the result of improved P acquisition.
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  • 4
    Publication Date: 2018
    Description: 〈span〉Mantle lithosphere heterogeneities are well documented, are ubiquitous, and have often been thought to control lithosphere-scale deformation. Here, we explore the influence of deep scarring in crustal deformation in three dimensions by considering the Ouachita orogeny in the southeastern United States, an example of a continental collision where mantle structure is present but not previously linked to the regional crustal tectonics. We present state-of-the-art continental compressional models in the presence of inherited three-dimensional lithospheric structure. Our models find that the surface expression of the Ouachita orogeny is localized by, and projected from, the controlling mantle scarring, in keeping with geological and geophysical observations. We are able to produce a large-scale arcuate orogeny with associated basin development appropriate to the Ouachita orogeny, alongside smaller-scale crustal faulting related to the region. This study offers a new and alternative hypothesis to the tectonic history of the Ouachita orogeny, with previous research having focused exclusively on crustal structures. The findings have broad implications, demonstrating the important potential role of the mantle lithosphere in controlling crustal dynamics and highlighting the requirement to consider deeper structure and processes when interpreting tectonic evolution of lithospheric-scale deformation.〈/span〉
    Print ISSN: 0091-7613
    Electronic ISSN: 1943-2682
    Topics: Geosciences
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  • 5
    Publication Date: 2015-12-02
    Description: Near-surface thrust fault splays and antithetic backthrusts at the tips of major thrust fault systems can distribute slip across multiple shallow fault strands, complicating earthquake hazard analyses based on studies of surface faulting. The shallow expression of the fault strands forming the Seattle fault zone of Washington State shows the structural relationships and interactions between such fault strands. Paleoseismic studies document an ~7000 yr history of earthquakes on multiple faults within the Seattle fault zone, with some backthrusts inferred to rupture in small (M ~5.5–6.0) earthquakes at times other than during earthquakes on the main thrust faults. We interpret seismic-reflection profiles to show three main thrust faults, one of which is a blind thrust fault directly beneath downtown Seattle, and four small backthrusts within the Seattle fault zone. We then model fault slip, constrained by shallow deformation, to show that the Seattle fault forms a fault propagation fold rather than the alternatively proposed roof thrust system. Fault slip modeling shows that back-thrust ruptures driven by moderate (M ~6.5–6.7) earthquakes on the main thrust faults are consistent with the paleoseismic data. The results indicate that paleoseismic data from the back-thrust ruptures reveal the times of moderate earthquakes on the main fault system, rather than indicating smaller (M ~5.5–6.0) earthquakes involving only the backthrusts. Estimates of cumulative shortening during known Seattle fault zone earthquakes support the inference that the Seattle fault has been the major seismic hazard in the northern Cascadia forearc in the late Holocene.
    Electronic ISSN: 1553-040X
    Topics: Geosciences
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  • 6
    Publication Date: 2014-03-15
    Description: The postorogenic collapse of the early Paleozoic Caledonian orogeny is well documented; however, several different plate tectonic models exist for the convergent phase involving closure of the Iapetus Ocean and the collision of Laurentia and Baltica. Receiver function analysis of 11 broadband seismometers along a 270 km transect in the East Greenland Caledonides reveals the existence of an east-dipping high velocity slab. Numerical modeling demonstrates that relict subducted and eclogitized crust is a plausible explanation. Thus, eastward subduction preceded subsequent west-dipping subduction during the formation of the East Greenland and Scandinavian Caledonides. This is a key constraint for understanding the Caledonian and continental margin evolution in the North Atlantic realm.
    Print ISSN: 0091-7613
    Electronic ISSN: 1943-2682
    Topics: Geosciences
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  • 7
    Publication Date: 2018
    Description: 〈span〉〈div〉Abstract〈/div〉Mantle lithosphere heterogeneities are well documented, are ubiquitous, and have often been thought to control lithosphere-scale deformation. Here, we explore the influence of deep scarring in crustal deformation in three dimensions by considering the Ouachita orogeny in the southeastern United States, an example of a continental collision where mantle structure is present but not previously linked to the regional crustal tectonics. We present state-of-the-art continental compressional models in the presence of inherited three-dimensional lithospheric structure. Our models find that the surface expression of the Ouachita orogeny is localized by, and projected from, the controlling mantle scarring, in keeping with geological and geophysical observations. We are able to produce a large-scale arcuate orogeny with associated basin development appropriate to the Ouachita orogeny, alongside smaller-scale crustal faulting related to the region. This study offers a new and alternative hypothesis to the tectonic history of the Ouachita orogeny, with previous research having focused exclusively on crustal structures. The findings have broad implications, demonstrating the important potential role of the mantle lithosphere in controlling crustal dynamics and highlighting the requirement to consider deeper structure and processes when interpreting tectonic evolution of lithospheric-scale deformation.〈/span〉
    Print ISSN: 0091-7613
    Electronic ISSN: 1943-2682
    Topics: Geosciences
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  • 8
    Publication Date: 2015-11-19
    Description: Plate tectonic reconstructions are usually constrained by the correlation of lineaments of surface geology and crustal structures. This procedure is, however, largely dependent on and complicated by assumptions on crustal structure and thinning and the identification of the continent-ocean transition. We identify two geophysically and geometrically similar upper mantle structures in the North Atlantic and suggest that these represent remnants of the same Caledonian collision event. The identification of this structural lineament provides a sub-crustal piercing point and hence a novel opportunity to tie plate tectonic reconstructions. Further, this structure coincides with the location of some major tectonic events of the North Atlantic post-orogenic evolution such as the occurrence of the Iceland Melt Anomaly and the separation of the Jan Mayen microcontinent. We suggest that this inherited orogenic structure played a major role in the control of North Atlantic tectonic processes.
    Print ISSN: 0091-7613
    Electronic ISSN: 1943-2682
    Topics: Geosciences
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  • 9
    Publication Date: 2017-04-01
    Description: The Evergreen basin is a 40-km-long, 8-km-wide Cenozoic sedimentary basin that lies mostly concealed beneath the northeastern margin of the Santa Clara Valley near the south end of San Francisco Bay (California, USA). The basin is bounded on the northeast by the strike-slip Hayward fault and an approximately parallel subsurface fault that is structurally overlain by a set of west-verging reverse-oblique faults which form the present-day southeastward extension of the Hayward fault. It is bounded on the southwest by the Silver Creek fault, a largely dormant or abandoned fault that splays from the active southern Calaveras fault. We propose that the Evergreen basin formed as a strike-slip pull-apart basin in the right step from the Silver Creek fault to the Hayward fault during a time when the Silver Creek fault served as a segment of the main route by which slip was transferred from the central California San Andreas fault to the Hayward and other East Bay faults. The dimensions and shape of the Evergreen basin, together with palinspastic reconstructions of geologic and geophysical features surrounding it, suggest that during its lifetime, the Silver Creek fault transferred a significant portion of the ~100 km of total offset accommodated by the Hayward fault, and of the 175 km of total San Andreas system offset thought to have been accommodated by the entire East Bay fault system. As shown previously, at ca. 1.5–2.5 Ma the Hayward-Calaveras connection changed from a right-step, releasing regime to a left-step, restraining regime, with the consequent effective abandonment of the Silver Creek fault. This reorganization was, perhaps, preceded by development of the previously proposed basin-bisecting Mount Misery fault, a fault that directly linked the southern end of the Hayward fault with the southern Calaveras fault during extinction of pull-apart activity. Historic seismicity indicates that slip below a depth of 5 km is mostly transferred from the Calaveras fault to the Hayward fault across the Mission seismic trend northeast of the Evergreen basin, whereas slip above a depth of 5 km is transferred through a complex zone of oblique-reverse faults along and over the northeast basin margin. However, a prominent groundwater flow barrier and related land-subsidence discontinuity coincident with the concealed Silver Creek fault, a discontinuity in the pattern of seismicity on the Calaveras fault at the Silver Creek fault intersection, and a structural sag indicative of a negative flower structure in Quaternary sediments along the southwest basin margin indicate that the Silver Creek fault has had minor ongoing slip over the past few hundred thousand years. Two earthquakes with ~M6 occurred in A.D. 1903 in the vicinity of the Silver Creek fault, but the available information is not sufficient to reliably identify them as Silver Creek fault events.
    Electronic ISSN: 1553-040X
    Topics: Geosciences
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  • 10
    Publication Date: 2016-10-08
    Description: Gravity and magnetic anomalies suggest that the Olympia structure beneath the southern Puget Lowland (western Washington State, U.S.) vertically displaces Eocene Crescent Formation strata. Northeast of the Olympia structure, middle Eocene Crescent Formation is beneath 4–6 km of Paleogene–Neogene and Quaternary strata of the Tacoma basin, whereas the Crescent Formation is exposed at the surface immediately to the south. Although numerous marine seismic reflection profiles have been acquired near the surface location of the Olympia structure as defined by potential field anomalies, its tectonic character remains enigmatic, in part because inlets of southern Puget Sound are too shallow for the collection of deep-penetration marine seismic profiles across the geophysical anomalies. To supplement existing shallow-marine data near the structure, we acquired 14.6 km of land-based seismic reflection data along a profile that extends from Crescent Formation exposed in the Black Hills northward across the projected surface location of the Olympia structure. The reflection seismic data image the Crescent bedrock surface to ~1 km depth beneath the southern Tacoma basin and reveal the dip on this surface to be no greater than ~10°. Although regional potential field data show a strong linear trend for the Olympia structure that implies folding over a blind thrust and/or bedrock juxtaposed against a weakly to nonmagnetic sediment section, high-resolution magnetic anomaly analysis along the land-based profile suggests that the structure is more complex. Overall, seismic and potential-field profiles presented in this study identify only minor shallow faulting within the projected surface location of the Olympia structure. We suggest that the mapped trace of the Olympia structure along the northern flank of the Black Hills, at least within the study area, is constrained by juxtaposed normal and reversely magnetized Crescent Formation units and minor tectonic deformation of Crescent Formation bedrock.
    Electronic ISSN: 1553-040X
    Topics: Geosciences
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