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Digitale Medien

Continuous-flow electrophoresis: a separation criterion applied to the separation of model proteins

Balmann, H.R.-d. ; Sanchez, V.

Amsterdam : Elsevier

Journal of Chromatography A 594 (1992), S. 351-359

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ISSN: 0021-9673

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Chemie und Pharmazie

Materialart: Digitale Medien

URL: http://linkinghub.elsevier.com/retrieve/pii/0021-9673(92)80349-Y

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2

Digitale Medien

Protein separation by continuous-flow electrophoresis in microgravity (1996)

Clifton, Michael J. ; Balmann, Hélène Roux-De ; Sanchez, Victor

Hoboken, NJ : Wiley-Blackwell

AIChE Journal 42 (1996), S. 2069-2079

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ISSN: 0001-1541

Schlagwort(e): Chemistry ; Chemical Engineering

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: During the IML-2 space shuttle mission, the RAMSES instrument was operated in the Spacelab module. This continuous-flow electrophoresis device performs separation and purification of protein solutions on a preparative scale. Samples containing artificial mixtures of pure proteins were used to test the capabilities of the device, and useful separations were obtained for proteins having a mobility difference of only 3 × 10-9 m2·V-1·s-1. Operating conditions that cannot be applied on earth were explored for two different sample concentrations, one of which is too high to allow treatment on earth. It agrees well with a previously published numerical model in that the main cause of loss in resolution in this process is the electrohydrodynamic spreading of the protein filaments.

Zusätzliches Material: 10 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/aic.690420727

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3

Digitale Medien

Conditions for purification of proteins by free-flow zone electrophoresis (1990)

Clifton, Michael J. ; Jouve, Nicolas ; Balmann, Hélène De ; [weitere]

Weinheim : Wiley-Blackwell

Electrophoresis 11 (1990), S. 913-919

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ISSN: 0173-0835

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie

Notizen: The separation of proteins by free-flow zone electrophoresis is generally impaired by a number of secondary effects which cause spreading of the protein streams with resultant loss in resolution. A strategy is outlined, based on experimental observations and numerical modeling, which allows the operating conditions and separation chamber dimensions to be chosen so as to obtain complete separation of two proteins of known mobility. This approach takes into account some dispersive phenomena such as molecular diffusion, electroosmosis and residence time gradients. In some cases, the right operating conditions cannot be achieved on earth and electrophoresis separations in microgravity may then be justified.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/elps.1150111106

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4

Digitale Medien

Continuous flow isoelectric focusing for purification of proteins (1998)

Soulet, Nathalie ; Roux-de Balmann, Hélène ; Sanchez, Victor

Weinheim : Wiley-Blackwell

Electrophoresis 19 (1998), S. 1294-1299

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ISSN: 0173-0835

Schlagwort(e): Isoelectric focusing ; Continuous free flow electrophoresis ; Purification ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie

Notizen: To estimate the feasibility of isoelectric focusing in a continuous mode, the conditions for obtaining stable and linear pH gradients were first investigated, using a mixture of carrier ampholytes. Then the operating parameters allowing the isoelectric focusing of bovine serum albumin as model protein were determined. A theoretical approach was used to find conditions minimizing the electrohydrodynamic effect, mainly responsible for protein dispersion, for the separation of bovine serum albumin and α-lactalbumin from bovine milk.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/elps.1150190813

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5

Digitale Medien

Purification of bioproducts by free-flow zone electrophoresis: Choice of processing parameters (1998)

Roux-De Balmann, Hélène ; Cerro, Rose-Marie ; Sanchez, Victor

Weinheim : Wiley-Blackwell

Electrophoresis 19 (1998), S. 1117-1126

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ISSN: 0173-0835

Schlagwort(e): Purification ; Free-flow electrophoresis ; Transport phenomena ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie

Notizen: Free-flow zone electrophoresis may be used to purify biological samples, due to differences in electrophoretic mobility, in absence of a matrix - most frequently a gel - thus enabling the biological integrity of even fragile molecules to be preserved. However, the process is more complicated than its principle suggests due to different transport phenomena interfering with electrophoretic migration, with the resultant separation depending both on separation effects and dispersive phenomena. The physical origin of the main effects involved was identified. Mathematical expressions were proposed to estimate the influence of the crescent effect and electrohydrodynamics on the process. In this paper, these equations are used to determine the minimum difference in electrophoretic mobility required for a separation to be achieved with respect to the processing parameters. A methodology is proposed which defines the conditions under which the difference in electrophoretic mobilities equals that calculated when considering the influence of dispersive phenomena. Optimized separations of the whey proteins lactoferrin and albumin, known to interact strongly, and the purification of a monoclonal antibody from a mouse ascitic fluid illustrate the approach.

Zusätzliches Material: 15 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/elps.1150190711

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6

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On Investment Consumption Modeling with Jump Process Extensions for Productive Sectors (2013)

Engler, Tina ; Balmann, Alfons

Springer

In: Journal of Optimization Theory and Applications. 2013; 167(3): 949-958. Published 2013 Sep 05. doi: 10.1007/s10957-013-0406-5.

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Publikationsdatum: 2013-09-05

Print ISSN: 0022-3239

Digitale ISSN: 1573-2878

Thema: Mathematik

Publiziert von Springer

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Sustainability, Vol. 8, Pages 584: The Rise of the Food Risk Society and the Changing Nature of the Technological Treadmill (2016)

Lioudmila Chatalova; Daniel Müller; Vladislav Valentinov; Alfons Balmann

MDPI Publishing

In: Sustainability

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Publikationsdatum: 2016-06-23

Beschreibung: Economic development of transition and developed countries is associated with increasingly unhealthy dietary habits among low-income population segments. Drawing on Ulrich Beck’s sociological theory of risk society, the present research note calls attention to the positive relation between national economic development and food risks that result in the rise of food-related diseases and healthcare costs. On this basis, we argue that the knowledge-intensive agribusiness may translate Cochrane’s technological treadmill into Beck’s risk treadmill that shifts a growing share of food-related healthcare costs from producers toward consumers, state, and the healthcare system. This argument motivates a novel research program dealing with the “food risk treadmill” that emerges in response to modern farming and agribusiness practices. Awareness of the food risk treadmill may help to streamline the development of agricultural science and to prevent it from being excessively dominated by the agricultural and food industry.

Digitale ISSN: 2071-1050

Thema: Energietechnik

Publiziert von MDPI Publishing

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Policy impact analysis in competitive agricultural markets: a real options approach (2013)

Feil, J.-H., Musshoff, O., Balmann, A.

Oxford University Press

In: European Review of Agricultural Economics

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Publikationsdatum: 2013-07-31

Beschreibung: In consequence of strong changes in general economic conditions, adjustments in the agricultural sector can be expected. To date, however, there are only few policy impact analyses on agricultural investments in a dynamic-stochastic context. The objective of this paper is to develop a real options market model which allows the impact assessment of different political schemes. The model combines genetic algorithms and stochastic simulation. Simulations of the model show that investment subsidies and production ceilings are preferable to price floors because the welfare is less reduced for a given stimulation of the willingness to invest.

Schlagwort(e): D81 - Criteria for Decision-Making under Risk and Uncertainty, Q12 - Micro Analysis of Farm Firms, Farm Households, and Farm Input Markets, Q18 - Agricultural Policy ; Food Policy

Print ISSN: 0165-1587

Digitale ISSN: 1464-3618

Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Wirtschaftswissenschaften

Publiziert von Oxford University Press

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9

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Post-Soviet cropland abandonment and carbon sequestration in European Russia, Ukraine and Belarus (2013)

Florian Schierhorn, Daniel Müller, Tim Beringer, Alexander V. Prishchepov, Tobias Kuemmerle, Alfons Balmann

Wiley

In: Global Biogeochemical Cycles

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Publikationsdatum: 2013-11-08

Beschreibung: [1] Widespread cropland abandonment occurred after the collapse of socialism across the former Soviet Union, but the rates and spatial patterns of abandoned lands are not well known. As a result, the potential of this region to contribute to global food production and estimates of the carbon sink developing on currently idle lands are highly uncertain. We developed a spatial allocation model that distributes yearly and sub-national sown area statistics to the most agriculturally suitable plots. This approach resulted in new, high-resolution (1 km 2 ) annual time series of cropland and abandoned lands in European Russia, Ukraine and Belarus from 1990 to 2009. A quantitative validation of the cropland map confirms the reliability of this dataset, especially for the most important agricultural areas of the study region. Overall, we found a total of 87 Mha of cropland and 31 Mha of abandoned cropland in European Russia, Ukraine and Belarus combined, suggesting that abandonment has been severely underestimated in the past. The abandonment rates were highest in European Russia. Feeding our new map dataset into the dynamic vegetation model LPJmL revealed that cropland abandonment resulted in a net carbon sink of 470 TgC for 1990 to 2009. Carbon sequestration was generally slow in the early years after abandonment, but carbon uptake increased significantly after approximately 10 years. Recultivation of older abandoned lands would be associated with high carbon emissions and lead to substantial amounts of carbon not being sequestered in vegetation formations currently developing on idle croplands. Our spatially and temporally explicit cropland abandonment data improve the estimation of trade-offs involved in reclaiming abandoned croplands and thus in increasing agricultural production in this globally important agricultural region.

Print ISSN: 0886-6236

Digitale ISSN: 1944-9224

Thema: Biologie , Chemie und Pharmazie , Geographie , Geologie und Paläontologie , Physik

Publiziert von Wiley im Namen von American Geophysical Union (AGU).

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