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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 12 (1996), S. 1677-1702 
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: No Abstract
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0749-503X
    Keywords: Candida albicans ; HIS4 ; complementation ; molecular biology tools ; topological marker ; amino acid biosynthesis general control ; PEX5 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have isolated the Candida albicans HIS4 (CaHIS4) gene by complementation of a his4-34 Saccharomyces cerevisiae mutant. The sequenced DNA fragment contains a putative ORF of 2514 bp, whose translation product shares a global identity of 44% and 55% to the His4 protein homologs of S. cerevisiae and Kluyveromyces lactis, respectively. Analysis of CaHIS4 sequence suggests that, similarly to S. cerevisiae HIS4, it codes for a polypeptide having three separate enzymatic activities (phosphoribosyl-AMP cyclohydrolase, phosphoribosyl-ATP pyrophosphohydrolase and histidinol dehydrogenase) which reside in different domains of the protein. A C. albicans his4 strain is complemented with this gene when using a C. albicans-S. cerevisiae-Escherichia coli shuttle vector, thus enabling the construction of a host system for C. albicans genetic manipulation. In addition, upstream of the sequenced CaHIS4 sequence, we have found the 3′-terminal half of a gene encoding a PEX5-like protein. The EMBL/DDJB/GenBank Accession Number of this sequence is AJ003115. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 3 Ill.
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  • 3
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Analysis of the initial interaction betweenEntamoeba histolytica trophozoites and the large intestine is impossible in humans and very difficult in experimental animals. To circumvent this obstacle we treated the luminal side of full-thickness rabbit colon segments mounted in Ussing-type chambers with trophozoite lysates of theE. histolytica HM1 virulent strain. Exposure to lysates for up to 90 min produced dose- and time-dependent effects on the colon, consisting of (a) increased decay rates for potential difference, short-circuit current, and transmural resistance and (b) mucosal damage ranging from vacuolation at the bases and shortening of epithelial cells to the loss of intercellular junctions, destruction of microvilli, and necrosis of interglandular epithelial zones. This acute model of intestinal amebiasis is sensitive, fast, and reliable.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract   One of the main drawbacks of experimental amebiasis is the lack of an adequate animal model for invasive intestinal lesions. Mongolian gerbils are useful because both intestinal and hepatic amebiasis can be produced experimentally with Entamoeba histolytica trophozoites. In this paper we show results obtained with in vivo and in vitro models of intestinal amebiasis in gerbils. We inoculated gerbils intracecally with monoxenic cultures of a highly virulent E. histolytica HM1:IMSS substrain. In the in vivo model an increase in mucus production was observed during the first 6 h of interaction. Microulcerative mucosal lesions appeared at 24–72 h postinoculation. Inflammatory infiltrate and edema of the lamina propria were associated with superficial foci of necrosis. At 96 h the cecal mucosa had an almost normal appearance and live amebas were no longer detected. In the in vitro model, early damage was detected in cecal strips mounted in Ussing chambers as a rapid fall in potential difference, short-circuit current, and transepithelial resistance that correlated with the extent of the microscopic lesions produced. The latter consisted of cellular edema and the appearance of small, translucent vacuoles at the base of epithelial cells. Further damage led to loss of intercellular junctions, destruction of interglandular epithelial cells, and edema of the lamina propria. The present results demonstrate that the gerbil is useful as an experimental model for the analysis of early stages of invasive intestinal amebiasis both in vivo and in vitro.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0749-503X
    Keywords: Firefly luciferase ; yeast expression ; gene reporter ; Saccharomyces cerevisiae ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The LUC gene coding for Photinus pyralis firefly luciferase was cloned in different yeast episomal plasmids in order to assess its possibilities as an in vivo reporter gene. Activity of the enzyme in transformed cells in vivo was measured by following light emission and assay conditions optimized in intact cells, with regard to oxygen concentration, temperature, cell concentration in assay mixtures and external ATP concentration. Among the factors tested, light emission was drastically influenced by the external pH in the assay (which resulted in a ten-fold amplification signal) and by substrate permeability. The growth phase of the cells was also important for the level of activity detected. Cloning of firefly luciferase gene under the control of different yeast-regulated promoters (ADH1, GAL1-10) enabled us to measure their strength which correlated well with previously described data. We conclude that firefly luciferase is an adequate gene reporter for the in vivo sensitive determination of gene expression and promoter strength in yeast.
    Additional Material: 5 Ill.
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  • 6
    Publication Date: 2019-03-14
    Description: The main objective of this study is to determine the influence of service quality on satisfaction of football spectators, looking scarcely investigated in the literature. The results, from a sample of 700 spectators at a football club of the Spanish First Division, reveal three main conclusions: (i) the quality of service of a football event is determined by both tangibles - infrastructure - as intangible - quality and delivery of equipment, and management of the board; (ii) the quality of positively affects service satisfaction experienced by the viewer part associated with football event; (iii) in overall satisfaction directly influences partial satisfaction associated with specific aspects of the sporting event and indirectly the quality of services provided. The study concludes with the main contributions, limitations and future research.
    Keywords: M31 ; ddc:330 ; Perceived quality ; Service ; Customer satisfaction ; Sport event ; Football
    Repository Name: EconStor: OA server of the German National Library of Economics - Leibniz Information Centre for Economics
    Language: Spanish
    Type: doc-type:article
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  • 7
    Publication Date: 2018-11-16
    Description: The study of the factors that have an influence on firm's export performance is one of the more fruitful lines of research in international marketing literature. However, there is no agreement among the researchers about which ones are the determinants that have an influence on the results of firms' exporting activity. In this sense, starting from the main reviewing studies on this topic, the purpose of this work consists on proposing a conceptual model that contains the main variables that have an influence on the export performance.
    Keywords: ddc:330 ; export Performance ; determinants ; international
    Repository Name: EconStor: OA server of the German National Library of Economics - Leibniz Information Centre for Economics
    Language: Spanish
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  • 8
    Publication Date: 2018-12-19
    Description: The preoccupation to consider the heterogeneity in firm behavior within an activity sector has been one of the main reasons for the search and identification of strategic groups, generating an inexhaustible debate in the scope of the strategic management. Nevertheless, this preoccupation has not been limited to identify firm archetypes or configurations, but also it has extended to the study of possible differences in performance between the identified groups. In this paper, we tried to answer to this double problem focusing in the scope of franchising. A data base including all the franshisor chains (664) that operated in Spain in 2005 has been elaborated. Results reveal the existence of five strategic groups (types of franchisors) perfectly differentiated, which are described from the strategic variables they define them. Also, the existence of differences between groups with regard to results is shown based on some performance measures (income, ordinary results and profit).
    Keywords: M10 ; M19 ; ddc:330 ; franchising ; franchisors ; strategic groups ; performance ; profit
    Repository Name: EconStor: OA server of the German National Library of Economics - Leibniz Information Centre for Economics
    Language: Spanish
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  • 9
    Publication Date: 2018-05-30
    Description: Most of articles that have recently appeared studying electronic service quality propose scales that are focused on measuring the quality of a particular service. However, could these scales be applied to measure the quality of other online services? In this situation, the main purpose of this study is to test the equivalence of a factor model to evaluate the quality of three services: online travel reservations, accommodation reservations and online ticketing. The results support the measurement equivalence of the measures. However, the importance of the dimensions varies across the services. In this sense, reliability is the most important factor in the evaluation of Websites of travel and accommodation reservations. Furthermore, in the evaluation of ticketing Websites, the higher weight factor is design.
    Keywords: ddc:650 ; electronic service quality ; measurement invariance ; multigroup ; confirmatory factor analysis ; Website
    Repository Name: EconStor: OA server of the German National Library of Economics - Leibniz Information Centre for Economics
    Language: English
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  • 10
    Publication Date: 2018-12-19
    Description: Franchising system continues its unstoppable advance worldwide, as in the most commercially developed countries as in those that have some more antiquated commercial structures. For that reason, nowadays, this is an important research topic in the business area. Franchising chains expand themselves through own and franchised units, in this context, this work intends to determine what kinds of units are more efficient. Own and franchised units are compared by the following indexes: sales per employee, sales per establishment and employees per establishment. As performances measures has been used: ROI (return on investment), incomes and ordinary results. This field of research has not been developed in Spain and scantly treated in the scientific international literature.
    Keywords: ddc:330 ; franchise system ; franchised units ; own units ; eficiency ; agency theory ; resource scarcity theory ; extension risk theory
    Repository Name: EconStor: OA server of the German National Library of Economics - Leibniz Information Centre for Economics
    Language: Spanish
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