ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
A novel lectin (SML-2) consisting of 138 amino acids was isolated from cyst merozoites of Sarcocystis muris and sequenced by Edman degradation and mass spectrometry. All 12 cysteinyl residues are involved in disulfide bridges, four of which are attributed to a characteristic pattern of cysteines as found in the so-called PAN-module superfamily. Crystals of SML-2 diffracting to 2.1 Å resolution at a synchrotron were grown by the hanging-drop vapour-diffusion technique. They belong to the space group P212121, with unit-cell parameters a = 53.6, b = 128.8, c = 158.2 Å and eight molecules in the asymmetric unit. SML-2 cocrystallized with Au galactose results in two different crystal forms. The first form is isomorphous with the native crystals and the second form adopts space group C2221, with unit-cell parameters a = 74.7, b = 82.0, c = 131.0 Å, and diffracts to 2.4 Å at a rotating-anode X-ray generator.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444901007284
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