ISSN:
0935-6304
Schlagwort(e):
Liquid chromatography, HPLC
;
HPLC columns
;
Packing procedure
;
Aqueous slurry
;
Zeta potential
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
Successful packing of high quality HPLC columns is dependent upon a number of parameters. Especially when using slurry packing techniques, the absence of conglomerates of particles in the slurry and the wettability of the particles are important. The method described in this paper fulfills these conditions, imparting a ξ potential to the 5 and 10 μm silica particles by applying a pH gradient. This results in repulsive forces between the particles and hence leads to a well-controlled monodisperse situation. Water is used as a slurry and displacement liquid; it combines the advantages of excellent wetting properties and non-toxicity.
Zusätzliches Material:
5 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/jhrc.1240050505
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