ISSN:
1432-2048
Schlagwort(e):
Calcium (enzyme activation)
;
1,3-β-Glucan synthase
;
Glycine (glucan synthase activation)
;
Plasma membrane
;
Polyamine
;
Polycation
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract Sucrose-density-gradient centrifugation and partitioning in a polyethylene glycol/dextran two-phase system were used to isolate plasmamembrane vesicles from microsomal preparations of soybean cell suspension cultures. Both methods resulted in the enrichment of the activity of a 1,3-β-glucan synthase which forms a polymer consisting of more than 99% of 1,3-linked glucose (callose). Digitonin increases the 1,3-β-glucan synthase activity in the various membrane fractions to a different degree, supporting the suggestion that this enzyme is vectorially arranged in the plasma membrane. The enzyme is greatly activated either by poly-l-ornithine or synergistically by Ca2+ and spermine, indicating that the same enzyme is affected and exhibits the regulatory properties necessary for callose synthesis.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00395076
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