Publikationsdatum:
2013-03-28
Beschreibung:
SAD-1 is a serine/threonine kinase which plays an important role in the regulation of both neuronal polarity and synapse formation inCaenorhabditis elegans. The kinase domain of SAD-1 fromC. eleganswas overexpressed inEscherichia coliBL21 (DE3) cells and purified to homogeneity using nickel–nitrilotriacetic acid metal-affinity, ion-exchange and gel-filtration chromatography. Diffraction-quality crystals were grown using the sitting-drop vapour-diffusion technique from a condition consisting of 1 MCAPSO pH 9.6, 10%(w/v) polyethylene glycol 3350. The crystals belonged to the monoclinic space groupC2, with unit-cell parametersa= 205.4,b= 57.1,c= 71.7 Å, β = 106.1°. X-ray diffraction data were recorded to 3.0 Å resolution from a single crystal using synchrotron radiation.
Print ISSN:
1744-3091
Thema:
Biologie
,
Chemie und Pharmazie
,
Geologie und Paläontologie
,
Physik
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