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  • 11
    Publication Date: 1996-10-01
    Print ISSN: 0003-2700
    Electronic ISSN: 1520-6882
    Topics: Chemistry and Pharmacology
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  • 12
    ISSN: 0935-6304
    Keywords: Monolith ; factor VIII ; affinity chromatography ; combinatorial peptide library ; peptide synthesis ; recombinant proteins ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: FVIII is a very complex molecule of great therapeutic significance. It is purified by a sequence of chromatographic steps including immunoaffinity chromatography. A peptide affinity chromatography method has been developed using peptides derived from a combinatorial library. Spot technology using cellulose sheets has been applied for this purpose. The dual positional scanning strategy was used for identification of the amino acids in random positions. Approximately 5000 possible candidates found in the first screening round were reduced to a panel of 36. Six candidates have been selected empirically. Five peptides seem to be directed against the light chain of FVIII, one peptide seems to be directed against the heavy chain. The peptides have been immobilized on conventional beaded material and CIM polymethacrylate monoliths. Much better performance with respect to capacity and selectivity has been observed with the monolithic material. Exposure of the ligand and its ensuing accessibility are responsible for these properties.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 13
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    Heidelberg University Publishing (heiUP)
    Publication Date: 2024-03-24
    Description: In this third volume of the “Online Handbook of Language Criticism – A European Perspective”, linguistic purism is being understood as both criticism of language usage on the level of parole and criticism of forms and structures of a language on the level of langue. The articles in this volume illustrate in comparative perspective in which constellations of languages linguistic purism in German, English, French, Italian, and Croatian was relevant in the past, and still is today, how the individual languages were and are affected by language-puristic activities, and which actors and discourses have formed and framed language-puristic activities in the different language cultures.
    Description: In dem vorliegenden Handbuchband wird Sprachpurismus als eine Kritik am Sprachgebrauch und als eine Kritik an verschiedenen Strukturen und Elementen einer Sprache verstanden. Es wird sprachvergleichend aufgezeigt, in welchen Sprachkonstellationen Sprachpurismus in der Vergangenheit sowie heute von Bedeutung ist und inwiefern die einzelnen Sprachkulturen von sprachpuristischen Aktionen betroffen waren bzw. sind. Außerdem wird auf wichtige Akteure und auf spezifische diskursive Zusammenhänge der einzelnen Sprachräume eingegangen.
    Keywords: Linguistic purism ; Language criticism ; German ; English ; French ; Italian ; Croatian ; Sprachpurismus ; Sprachkritik ; Deutsch ; Englisch ; Französisch ; Italienisch ; Kroatisch ; thema EDItEUR::C Language and Linguistics
    Language: German , English , French , Italian , Croatian
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  • 14
    Publication Date: 2022-01-31
    Description: This volume presents the findings of a European research network on the comparative description of German. The key area of exploration was corpus-assisted comparative research on grammatical variations in the left sentence periphery. The compendium provides insight into topological and information structural aspects of German in comparison to the French, Norwegian, Polish, and Hungarian languages.
    Keywords: P1-1091 ; Contrastive Syntax ; Informational Structure ; Prefield
    Language: German
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