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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 132 (1982), S. 365-371 
    ISSN: 1432-072X
    Keywords: Pyruvate formate-lyase ; Anaerobiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It is shown here that a plasmid (p29) derived from the transducing phage λaspC2 (Christiansen and Pedersen 1981) codes for pyruvate formate-lyase. The identity of the 80 kilodaltons (kd) gene product of plasmid p29 with the pyruvate formate-lyase polypeptide was proven (i) by comigration of the gene product expressed in the maxicell system with purified enzyme on O'Farrell gels, and (ii) by comparison of the peptide maps obtained from limited proteolysis. In vivo the 80 kd form of the enzyme was proteolytically converted to a 78 kd polypeptide. The two polypeptides (80 kd and 78 kd) and their charge isomers present in purified enzyme preparations are therefore products of a single gene. Aerobically grown cells of Escherichia coli contained a basal level of pyruvate formate-lyase which was derepressed 5-to 10-fold under anaerobiosis. Derepression also occurred during anaerobic growth on glycerol plus fumarate. Presence of plasmid p29 caused overproduction of pyruvate formatelyase, 11-fold upon anaerobic growth on glucose, 14-fold upon aerobic growth on glucose and 33-fold upon aerobic growth at the expense of D-lactate.
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  • 12
    ISSN: 1432-072X
    Keywords: Redox control ; Enterobacteriaceae ; Anaerobiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mutants of Escherichia coli were isolated in which transcription of the structural genes for hydrogenase (hyd) and for one of the components of formate dehydrogenase (fdh) (of the formate hydrogen-lyase complex) is coupled with that of the lacZ gene. They were — together with lac fusions of the nifH and nifL genes from Klebsiella — used to study regulation by redox control, of the expression of the respective structural genes. The following results were obtained: (i) β-galactosidase synthesis was fully repressed in the presence of O2 or nitrate (anaerobically), and induced in the absence of an external electron acceptor. Fumarate as terminal electron acceptor only marginally affected nif expression and partially repressed hyd and fdh expression. Redox control of the synthesis of hydrogenase and formate dehydrogenase, therefore, (as well as that of nif) acts at the level of transcription; the size of the redox potential seems to be correlated with the amount of repression; (ii) β-galactosidase synthesis in the hyd:: lac and fdh::lac fusion strains is induced by formate. At high concentrations formate reverses the repression by nitrate and fumarate but not that by oxygen.
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  • 13
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 10 (1981), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 14
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 158 (1989), S. 99-104 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Nuclear Physics, Section A 379 (1982), S. 330-348 
    ISSN: 0375-9474
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 16
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 158 (1989), S. 99-104 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 420 (2002), S. 622-623 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Gas hydrates are an ice-like form of water that has cavities containing gas — usually methane. They exist in vast quantities beneath the ocean floor in certain areas, especially continental margins, where the methane is generated mostly from the bacterial breakdown of organic matter, and they ...
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  • 18
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 73 (1998), S. 31-33 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Methyl radicals from a methane electron cyclotron resonance plasma are measured quantitatively at the sample position by ionization-threshold mass spectrometry (ITMS). The absolute fluxes are determined by calibrating the CH3 ITMS results with those of methane, taking into account the published energy-dependent cross sections for the ionization of CH3 and CH4, respectively. The measured CH3 radical fluxes are on the order of some 1015 cm−2 s−1, which is in accordance with recent modeling results. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 19
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 51 (2004), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Perkinsus species are parasitic protozoa of mollusks, currently classified within the Perkinsozoa, a recently established phylum that is basal to the Apicomplexa and Dinozoa. Ribosomal RNA (rRNA) genes and their intergenic spacers have been used to support the taxonomy of Perkinsus species, the description of new species, and to develop molecular probes for their detection and identification. We previously described ultrastructure, behavior in culture, and partial sequence of the rRNA locus of a Perkinsus species isolated from the baltic clam Macoma balthica. The rRNA genes and intergenic spacers of this Perkinsus isolate differed from those described in the currently accepted species to a degree that led to its designation as a new species, Perkinsus andrewsi. In this study, we identify an additional rRNA gene unit (rRNA-B) in the P. andrewsi holotype, and report the complete sequences of both rRNA gene units. Except for the 5. 8S, all regions of the rRNA-B gene unit exhibited sequence differences from that initially described (rRNA-A). Each rRNA gene unit is arranged in a “head-to-tail” tandem repeat. This is the first report demonstrating two distinct rRNA units in a Perkinsus species.
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  • 20
    ISSN: 1525-1314
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: The South Karakorum margin, east of the Himalayan syntaxis, consist of an E–W elongated zone of young (10–3 Ma) high-grade metamorphic rocks (M2) and related migmatitic domes. This late tectono-metamorphic event post-dates the Palaeogene (55–37 Ma) phase of thickening of the belt featured by NW–SE structures and associated M1 amphibolite facies metamorphism (0.7 GPa, 700 °C). This M2 metamorphism is characterised by low-pressure, high-temperature conditions coeval with migmatite formation in response to a thermal increase of c. 150 °C compared to M1, culminating at a temperature of c. 770 °C and a pressure of 0.5–0.6 GPa. Rapid exhumation of migmatitic domes, at a rate of 5 mm yr−1, was accommodated by vertical extrusion, in the core of E–W crustal-scale folds. These crustal-scale folds formed in response to N–S syn-collisional shortening and were enhanced by thermal weakening of the migmatised continental crust.M2 metamorphism is spatially and temporarily associated with granitoids showing a mantle affinity, firmly suggesting that this could be the advective heat source for the granite and syenite generation and the subsequent migmatisation of the mid-crustal level. Such relationships between a mantle-related magmatism and a high-temperature metamorphism in a convergent shortening context are suggestive of the breakoff of the subducted Indian slab since 20 Ma.
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