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  • 11
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 263 (1976), S. 145-146 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Three fibre-blast lines were used: human lung fibroblasts (from 13-weekembryos) obtained from Gibco-Biocult (Paisley, Scotland); human skin fibroblasts (from 14?16-week embryos) and rat skin fibroblasts (from 16?18-day embryos) cultured in this laboratory. All cell lines were grown in air on glass ...
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  • 12
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 214 (1967), S. 715-716 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] It seems that early attempts to obtain colonies of this organism by streaking failed because of the method used to prepare the Repaskes agar plates. This organism apparently does not grow on Repaskes medium when all the ingredients are autoclaved together, or if the plates are held for 24 h before ...
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  • 13
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Organometallic Chemistry 178 (1979), S. C43-C49 
    ISSN: 0022-328X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 14
    ISSN: 1525-1314
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: The central part of the Carolina terrane in western South Carolina comprises a 30 to 40 km wide zone of high grade gneisses that are distinct from greenschist facies metavolcanic rocks of the Carolina slate belt (to the SE) and amphibolite facies metavolcanic and metaplutonic rocks of the Charlotte belt (to the NW). This region, termed the Silverstreet domain, is characterized by penetratively deformed felsic gneisses, granitic gneisses, and amphibolites. Mineral assemblages and textures suggest that these rocks formed under high-pressure metamorphic conditions, ranging from eclogite facies through high-P granulite to upper amphibolite facies.Mafic rocks occur as amphibolite dykes, as metre-scale blocks of coarse-grained garnet-clinopyroxene amphibolite in felsic gneiss, and as residual boulders in deeply weathered felsic gneiss. Inferred omphacite has been replaced by a vermicular symplectite of sodic plagioclase in diopside, consistent with decompression at moderate to high temperatures and a change from eclogite to granulite facies conditions. All samples have been partially or wholly retrograded to amphibolite assemblages. We infer the following P-T-t history: (1) eclogite facies P-T conditions at ≥ 1.4 GPa, 650–730 °C (2) high-P granulite facies P-T conditions at 1.2–1.5 GPa, 700–800 °C (3) retrograde amphibolite facies P-T conditions at 0.9–1.2 GPa and 720–660 °C. This metamorphic evolution must predate intrusion of the 415 Ma Newberry granite and must postdate formation of the Charlotte belt and Slate belt arcs (620 to 550 Ma).Comparison with other medium temperature eclogites and high pressure granulites suggests that these assemblages are most likely to form during collisional orogenesis. Eclogite and high-P granulite facies metamorphism in the Silverstreet domain may coincide with a ≈570–535 Ma event documented in the western Charlotte belt or to a late Ordovician-early Silurian event. The occurrence of these high-P assemblages within the Carolina terrane implies that, prior to this event, the western Carolina terrane (Charlotte belt) and the eastern Carolina terrane (Carolina Slate belt) formed separate terranes. The collisional event represented by these high-pressure assemblages implies amalgamation of these formerly separate terranes into a single composite terrane prior to its accretion to Laurentia.
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  • 15
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    In:  CASI
    Publication Date: 2016-06-07
    Description: Various techniques and methods in fluid management are discussed. Propellant on-orbit transfer efficiency from the space shuttle orbiter to orbital transfer vehicle is discussed. Techniques such as refueling, residuals recovery and propellant storage are described.
    Keywords: SPACE TRANSPORTATION
    Type: Satellite Serv. Workshop, Vol. 1; p 381-405
    Format: application/pdf
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  • 16
    Publication Date: 2016-06-07
    Description: The Apollo 13 anomaly provided considerable impetus for a variety of types of cryogenic and ignition tests. The logic of the various test program designs, the test techniques, and their final impact upon the investigation findings are described. In addition, several test programs initiated to determine the thermal performance and general performance characteristics of the redesigned Apollo 14 cryogenic storage system are presented.
    Keywords: PHYSICS, GENERAL
    Type: MSC Cryog. Symp. Papers; p 433-454
    Format: application/pdf
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  • 17
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The feasibility of using scanning electron microscopy (SEM) to identify the position of specific DNA sequences was examined using a Y chromosome ‘specific’ probe (pHY2.1). Tests were carried out on chromosome spreads hybridizedin situ with biotinylated pHY2.1. Chromosomal sites of hybridization of the probe were localized by an indirect immunohistochemical procedure which resulted in a gold product which could be amplified by silver precipitation. In the SEM, the specific location of the probe was easily identified due to the enhanced signal produced by the gold—silver complex. The probe was localized both on the long arm of the Y chromosome and within interphase nuclei. It was found that SEM was more sensitive than light microscopy since the probe could be identified without silver amplification. With refinements to the technique, SEM could provide a useful method for high resolution localizing of unique DNA sequences (i.e. single copy genes).
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  • 18
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary It is generally assumed that nucleic acid association duringin situ hybridization reactions is similar to that of nucleic acid association in solution. This assumption has been investigated by detecting closely homologous human papillomavirus types 6 and 11 byin situ hybridization as a model for the evaluation of stringency conditions in clinical biopsies. By examining matched and mismatched, labelled and target sequences under various stringency conditions, empirical DNA-DNA stability curves and their derivative equations for tissue melting temperatures (Tmt) were derived. The corresponding values for Tmt are 10–20°C higher than their solution equivalents. These data, supported by polymerase chain reaction experiments, demonstrate that closely homologous viral DNAs cross linked in tissue by formaldehyde fixation do not interact with the corresponding labelled probes as predicted from solution kinetic equations. This not only has theoretical implications but is also relevant to the accuracy of clinical diagnostic testing.
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  • 19
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The parameter Tmt has been defined by non-isotopic in situ hybridization and describes the tissue melting temperature (Tmt) of human papillomavirus (HPV) DNA sequences. In this study, multiple in situ hybridization signals for HPV types 16, 31 and 33 in individual archival biopsies hybridized with genomic probes are shown by polymerase chain reactions to be due to cross-hybridization of probe sequences to a single tissue target. Tmt is independent of viral type but depends on the homology between probe and target when using nick-translated whole genomic probes. The difference between Tm and Tmt is not due to the presence of viral capsid protein. Multiple HPV signals in archival material should not therefore be interpreted as indicative of multiple HPV infection unless adequate stringency conditions have been employed or they are present in morphologically distinct areas of the biopsy. Furthermore, extrapolation of calculated DNA homologies to non-isotopic in situ hybridization analysis may not be appropriate. A hybridization signal does not imply probe and target identity: this has implications for HPV typing in clinical material.
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  • 20
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The experimentally derived parameter Tmt (tissue Tm) was defined previously to describe the end-point used for evaluation of the stringency of non-isotopic in situ hybridization and was found to differ from the theoretical melting temperature (Tm) for several HPV types. In this paper, the reasons for this discrepancy were investigated by performing a series of experiments with a variety of probes for both human genomic and integrated viral sequences in isolated and cultured normal and abnormal cells in addition to paraffin-embedded material. Tmt was shown to be dependent on several parameters of probe and target, and on the sensitivity of the detection system used but was not affected by aldehyde fixation or paraffin wax embedding under optimal conditions of nucleic acid unmasking. These data support the hypothesis that differences between Tmt and Tm may be due to the use of a different end-point for in situ hybridization analysis rather than biochemical alteration of DNA-DNA interactions in intact cells. Appropriate stringency conditions should therefore be determined by experiment rather than calculated theoretically for gene evaluation in cells and tissues.
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