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  • 11
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 25 (2002), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Causes of variation in cold hardiness in the autumn were assessed among closely related, fast-growing clones of willow of northern/continental and southern/maritime origins, under controlled regimes and natural conditions. Cold hardiness was assessed by controlled freezing followed by injury analysis, based on measurements of chlorophyll fluorescence (stems) and electrolyte leakage (leaves). During growth at a given temperature, the cold hardiness of the clones' stems was negatively correlated with their rate of growth. This apparently phenotypic variation was independent of temperature and, hence, the absolute growth rate. At later stages, cold hardiness of stems varied mainly with respect to genetic differences in the timing and rate of cold hardening. Cold hardening began up to 7 weeks earlier in northern/continental clones, and their rates of hardening in cool temperature regimes were up to three times higher than in southern/maritime clones. Ranking of clones with respect to rates was essentially the same whether natural or abrupt reductions of day length were used to trigger cold hardening. Results closely agreed with those of a previous field trial. Comparisons of rates at cool and warm temperatures suggest that cold hardening became increasingly dependent on cool temperatures with time. Increasing sucrose-to-glucose ratios, and especially dry-to-fresh weight ratios, paralleled early cold hardening. Before leaves were shed in the autumn they underwent cold hardening in parallel with stems, eventually allowing them to tolerate temperatures down to −10 °C.
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  • 12
    ISSN: 1573-8477
    Keywords: apical dominance ; Gentianella ; grazing tolerance ; herbivory ; meristem allocation ; overcompensation ; shoot architecture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Meristem allocation models suggest that the patterns of compensatory regrowth responses following grazing vary, depending on (i) the number of latent meristems that escape from being damaged, and (ii) the activation sensitivity of the meristems in relation to the degree of damage. We examined the shape of compensatory responses in two late-flowering populations (59°20′N and 65°45′N) of the field gentian. Plants of equal initial sizes were randomly assigned to four treatment groups with 0, 10, 50 and 75% removal of the main stalk. The plants were clipped before flowering, and their performance was studied at the end of the growing season. The northern population showed a linear decrease in shoot biomass and fecundity with increasing biomass removal, while the response in the southern population was quadratic with maximum performance at the damage level of 50% clipping. This nonlinear shape depended upon the activation sensitivity of dormant meristems in relation to their position along the main stem. The highest plant performance was achieved by inflicting intermediate damage which induced regrowth from basally located meristems. In contrast, the topmost branches took over the dominance role of the main stem after minor apical damage (10% clipping). Consequently, the breakage of apical dominance is a necessary precondition of vigorous regrowth in this species. However, compensation in the field gentian is unlikely to be a mere incidental by-product of apical dominance. The ability to regrow from basally located meristems that escape from being damaged by grazing may well be a sign of adaptation to moderate levels of shoot damage.
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  • 13
    ISSN: 1573-8477
    Keywords: browsing ; compensatory regrowth ; Gentianella campestris ; plasticity ; quantitative genetics ; tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the framework of phenotypic plasticity, tolerance to browsing can be operationally defined as a norm of reaction comparing plant performance in undamaged and damaged conditions. Genetic variation in tolerance is then indicated by heterogeneity in the slopes of norms of reaction from a population. We investigated field gentian (Gentianella campestris) tolerance to damage in the framework of phenotypic plasticity using a sample of maternal lines from natural populations grown under common garden conditions and randomly split into either a control or an artificial clipping treatment. We found a diversity of tolerance norms of reaction at both the population and family level: the impacts of clipping ranged from poor tolerance (negative slope) to overcompensation (positive slope). We detected heterogeneity in tolerance norms of reaction in four populations. Similarly, we found a variety of plastic architectural responses to clipping and genetic variation in these responses in several populations. Overall, we found that the most tolerant populations were late flowering and also exhibit the greatest plastic increases in node (meristem) production in response to damage. We studied damage-imposed natural selection on plasticity in plant architecture in 10 of the sampled populations. In general, there was strong positive direct selection on final number of nodes for both control and clipped plants. However, the total selection on nodes (direct + indirect selection) within each treatment category depended heavily on the frequency of damage and cross-treatment genetic correlations in node production. In some cases, strong correlated responses to selection across the damage treatment led to total selection against nodes in the more rare environment. This could ultimately lead to the evolution of maladaptive phenotypes in one or both of the treatment categories. These results suggest that tolerance and a variety of architectural responses to damage may evolve by both direct and indirect responses to natural selection. While the present study demonstrates the potential importance of cross-treatment genetic correlations in directing the evolution of tolerance traits, such as branch or node production, we did not find any strong evidence of genetic trade-offs in candidate tolerance traits between undamaged and damaged conditions.
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 51 (1977), S. 461-495 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract On the basis of field and particle observations, it is suggested that a bright auroral display is a part of a magnetosphere-ionosphere current system which is fed by a charge-separation process in the outer magnetosphere (or the solar wind). The upward magnetic-field-aligned current is flowing out of the display, carried mainly by downflowing electrons from the hot-particle populations in the outer magnetosphere (the ambient cold electrons being depleted at high altitudes). As a result of the magnetic mirroring of these downflowing current carriers, a large potential drop is set up along the magnetic field, increasing both the number flux and the kinetic energy of precipitating electrons. It is found that this simple basic model, when combined with wave-particle interactions, may be able to explain a highly diversified selection of auroral particle observations. It may thus be possible to explain both ‘inverted-V’ events and auroral rays in terms of a static parallel electric field, and the electric field may be compatible with a strongly variable pitch-angle distribution of the precipitating electrons, including distributions peaked at 90° as well as 0°. This model may also provide a simple explanation of the simultaneous precipitation of electrons and collimated positive ions.
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Space science reviews 80 (1997), S. 305-323 
    ISSN: 1572-9672
    Keywords: Magnetotail ; Plasma Sheet ; Low Latitude Boundary Layer ; Solar Wind Entry ; Tail Plasma Flows ; Substorms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Energetic (0.1-16 keV/e) ion data from a plasma composition experiment on the ISEE-1 spacecraft show that Earth's plasma sheet (inside of 23 RE) always has a large population of H+ and He++ ions, the two principal ionic components of the solar wind. This population is the largest, in terms of both number density and spatial thickness, during extended periods of northward interplanetary magnetic field (IMF) and is then also the most "solar wind-like" in the sense that the He++/H+ density ratio is at its peak (about 3% on average in 1978 and 79) and the H+ and He++ have mean (thermal) energies that are in the ratio of about 1:4 and barely exceed the typical bulk flow energy in the solar wind. During geomagnetically active times, associated with southward turnings of the IMF, the H+ and He++ are heated in the central plasma sheet, and reduced in density. Even when the IMF is southward, these ions can be found with lower solar wind-like energies closer to the tail lobes, at least during plasma sheet thinning in the early phase of substorms, when they are often seen to flow tailward, approximately along the magnetic field, at a slow to moderate speed (of order 100 km s-1 or less). These tailward flows, combined with the large density and generally solar wind-like energies of plasma sheet H+ and He++ ions during times of northward IMF, are interpreted to mean that the solar wind enters along the tail flanks, in a region between the lobes and the central plasma sheet, propelled inward by ExB drift associated with the electric fringe field of the low latitude magnetopause boundary layer (LLBL). In order to complete this scenario, it is argued that the rapid (of order 1000 km s-1) earthward ion flows (mostly H+ ions), also along the magnetic field, that are more typically the precursors of plasma sheet "recovery" during substorm expansion, are not proof of solar wind entry in the distant tail, but may instead be a time-of-flight effect associated with plasma sheet redistribution in a dipolarizing magnetic field.
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  • 16
    ISSN: 1573-6822
    Keywords: diethylnitrosamine ; EGF receptor ; enzyme-altered foci ; hepatocytes ; p53 ; TGF-α
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Previous reports indicate that the expression of transforming growth factor α (TGF-α) is increased in enzyme-altered foci (EAF) arising in livers of rats treated with a carcinogen. Here we have investigated the effects of TGF-α on EAF cells in vitro. Hepatocytes were isolated from rats that had received repeated treatment with diethylnitrosamine (DEN) and whose livers contained glutathione S-transferase P (GST-P)-positive EAF. Primary cultures of GST-P-positive and GST-P-negative hepatocytes were exposed to TGF-α. TGF-α (20–40 ng/ml) increased DNA replication in the GST-P-negative, but not in the GST-P-positive cells. Furthermore, it was shown that this effect on GST-P-negative cells could be blocked by p53 antisense oligonucleotides. We conclude that EAF hepatocytes do not respond to TGF-α in vitro. This lack of response may reflect the attenuated expression of p53 in these cells. These data corroborate previous findings that, in response to DNA damage, many EAF hepatocytes do not accumulate p53.
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  • 17
    ISSN: 1572-9672
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The science objectives of the Toroidal Imaging Mass-Angle Spectrograph (TIMAS) are to investigate the transfer of solar wind energy and momentum to the magnetosphere, the interaction between the magnetosphere and the ionosphere, the transport processes that distribute plasma and energy throughout the magnetosphere, and the interactions that occur as plasma of different origins and histories mix and interact. In order to meet these objectives the TIMAS instrument measures virtually the full three-dimensional velocity distribution functions of all major magnetospheric ion species with one-half spin period time resolution. The TIMAS is a first-order double focusing (angle and energy), imaging spectrograph that simultaneously measures all mass per charge components from 1 AMU e−1 to greater than 32 AMU e−1 over a nearly 360° by 10° instantaneous field-of-view. Mass per charge is dispersed radially on an annular microchannel plate detector and the azimuthal position on the detector is a map of the instantaneous 360° field of view. With the rotation of the spacecraft, the TIMAS sweeps out very nearly a 4π solid angle image in a half spin period. The energy per charge range from 15 eV e−1 to 32 keV e−1 is covered in 28 non-contiguous steps spaced approximately logarithmically with adjacent steps separated by about 30%. Each energy step is sampled for approximately 20 ms;14 step (odd or even) energy sweeps are completed 16 times per spin. In order to handle the large volume of data within the telemetry limitations the distributions are compressed to varying degrees in angle and energy, log-count compressed and then further compressed by a lossless technique. This data processing task is supported by two SA3300 microprocessors. The voltages (up to 5 kV) for the tandem toroidal electrostatic analyzers and preacceleration sections are supplied from fixed high voltage supplies using optically controlled series-shunt regulators.
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  • 18
    ISSN: 1572-9672
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 19
    ISSN: 1572-9672
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
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  • 20
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 208 (1997), S. 45-69 
    ISSN: 1615-6110
    Keywords: Gentianaceae ; Gentianella amarella ; G. campestris ; Seasonal differentiation ; seasonal ecotypes ; flowering phenology ; germination ; reproductive isolation ; environmental factors ; evolutionary history
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 18 populations of the grassland biennialsGentianella amarella andG. campestris were cultivated to clarify the genetical vs. environmental components of the flowering phenology, and the reproductive isolation caused by seasonal differentiation. The influence of some environmental factors was tested. The seasonal variation persisted in cultivation, and the plants could normally be assigned to distinct aestival or autumnal groups, with no reproductive contact. Flowering phenology was affected by environmental factors, but not to such an extent that the reproductive isolation was broken. The observed phenological variation was not reflected by a corresponding variation in present management practices. Management history and possible non-anthropogenic factors are discussed as alternative explanations.
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