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  • 11
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 41 (1992), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The infection of three resistant and two susceptible inbred lines of Pisum sativum by Mycosphaerella pinodes is described for the first time. Two types of resistance, one expressed in epicotyls and one in leaves, were found in all three resistant lines. On epicotyls of susceptible lines, abundant appressoria and penetrations occurred after a short period of hyphal growth. On epicotyls of resistant lines, hyphae grew extensively but rarely formed appressoria, and these failed to penetrate the cuticle. Attempted penetration was associated with the rapid death of 2–6 epicotyl cells, resembling a hypersensitive reaction. In contrast, resistance of leaves, which was only expressed after penetration, involved localization of the fungus by a mechanism involving delayed leaf cell death. It is suggested that a combination of these two types of resistance might provide effective protection against M. pinodes.
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  • 12
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 34 (1985), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In growth room regimes arranged to simulate field conditions which coincide with natural infection of oilseed rape by Leptosphaeria maculans, leaf inoculation resulted in systemic infection. After colonizing intercellular spaces in the spongy mesophyll of the lamina, the fungus reached a vascular strand and spread down the petiole mainly in xylem vessels or between cells of the xylem parenchyma and cortex, eventually invading and killing cells of the stem cortex and causing the stem canker symptom. The intercellular systemic phase of growth, which was biotrophic and virtually sytnptomless, occurred under a wide range of temperatures.
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  • 13
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 43 (1994), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The A and B groups (aggressive and non-aggressive) of Leptosphaeria maculans were compared in studies of host range, infection phenotypes and epidemiology. Isolates of both groups infected a wide range of cruciferous hosts including Brassica napus, B. rapa, B. oleracea, B. juncea, B. carinata. B. nigra, Thlaspi arvense and Raphanus sativus. On cotyledons, B-group isolates were generally more aggressive than A-group isolates, causing local lesions and subsequent systemic invasion of the majority of test species. On susceptible stems, A-group isolates caused cortical lesions; B-group isolates, if they reached the stem, usually caused pith lesions with no external symptoms. In a susceptible line of B. napus, CrGC5, systemic infections of the leaf and petiole were similar with both groups, but the B-group rarely entered the stem to form cortical lesions or cankers. At two different field locations, natural epidemics caused by the two groups on oilseed rape leaves were similar in overall pattern, B-group lesions occurred slightly later but the incidence increased more rapidly and reached a maximum slightly earlier than that of the A group. The two groups differed markedly in stem infection patterns. Infection of the cortex near the base of the stem and the development of a typical stem canker was caused entirely by the A group. However the B group often caused considerable damage to the pith. Superficial chlorotic lesions on stems and inflorescences were mainly attributable to the B group. In view of the stem pith infection by the B group without external symptoms, its importance on oilseed rape may have been underestimated previously.
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  • 14
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 36 (1987), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In a series of growth room experiments in which leaves of Brassica napus var. oleifera were inoculated with ascospores or pycnidiospores of Leptosphaeria maculans successful infections progressed through three consecutive phases. Initial establishment in the mesophyll was succeeded by a phase of intercellular exploration, when hyphal proliferation was highly variable and host cell necrosis always ensued, and then by a systemic phase when hyphae were consistently sparse. Host cells associated with the hyphal front were capable of autofluorescence, accumulation of vital stains and plasmolysis, indicating that they were viable and that the pathogen was biotrophic throughout this sequence. During either of the first two phases permanent fungistatic containment, involving the formation of vesicles by disintegration of the hyphae, often occurred. Localization at the first phase was symptomless; at the second it was signified by a lesion with a clearly defined margin.There was a negative correlation between biotrophic potential and necrotrophic potential of three pathogenic isolates, on both the moderately susceptible cultivar Primor and the resistant cultivar Jet Neuf. As leaves aged, a progressively larger proportion of infections failed to become systemic. With increasing inoculum load, symptomless localization of infection diminished, the phase of necrosis extended, and the probability of irreversible systemic development increased.
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  • 15
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 36 (1987), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Development of stem cankers in seedlings of Brassica napus var. oleifera inoculated with Leptosphaeria maculans was studied in growth cabinets. As the pathogen progressed from the petiole into the stem, hyphae invaded parenchyma cells for the first time, initiating a necrotrophic phase which resulted in the formation of a lesion. Ultimate containment of the pathogen was associated with a sequence of host reactions including lignification, cambium formation and callose deposition at the perimeter of the lesion. Two isolates of L. maculans differed in the pattern of stem infection. One was confined to the cortex and the other passed through the leaf gap into the pith. Variation in response between cultivars Rapora, Primor and Jet Neuf modified these two colonization patterns but did not eliminate the main differences. There were qualitative and quantitative differences in host cell reactions. The significance of these findings is discussed in relation to differences in resistance of the cultivars in the field.
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  • 16
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 35 (1986), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: When a range of genotypes of the genus Pisum was inoculated with Ascochyta pisi some remained uninfected or symptomless whereas the reactions of others ranged from a hypersensitive resistance through various levels of susceptibility to almost lethal infection. This variation, categorized on a six-class scale, was used to describe the reactions of 57 isolates of A. pisi collected from pea crops widely distributed in Britain when inoculated on to 14 differential lines of Pisum. Five major pathotype groups were distinguished and a set of lines nominated as standard differential hosts.
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  • 17
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 16 (1967), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Common scab (Streptomyces scabies) on ‘Majestic’ potato tubers was checked by irrigation maintaining the soil at saturation (‘field capacity’) throughout tbe growing season more than it was by irrigating only before the first tubers had grown to 1/4-1/2 in. diameter.The stage of the tubers when irrigation was started or stopped affected the distribution of scab lesions on their surfaces and infections were rare on tissue formed when soil was saturated.
    Type of Medium: Electronic Resource
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  • 18
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 112 (1994), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Six accessions belonging to four subspecies of Brassica rapa, including three accessions of B. rapa subsp. sylvestris, were crossed with B. oleracea subsp. alboglabra in order to develop a series of synthetic B. napus lines with a common C genome but contrasting A genomes. Different A genomes had significant effects on the efficiency of B. napus resynthesis and the sexual compatibility of the synthetic lines with oilseed rape cultivars. The synthetic lines were used to investigate the effect of A genome substitution on the resistance of B. napus to infection by Leptosphaeria maculans, and to explore the potential for the use of wild forms of B. rapa in oilseed rape breeding programmes. Synthetic lines derived from two wild accessions of B. rapa, and their F1 hybrids with oilseed rape cultivars, expressed high levels of resistance to L. maculans in glasshouse experiments. One of these lines also expressed high levels of resistance in field experiments in England and Australia when exposed to a genetically diverse pathogen population. All other synthetic lines and cultivars were highly susceptible in both glasshouse and field experiments. F1 hybrids between oilseed rape cultivars and synthetic lines derived from B. rapa subsp. chinensis were significantly more susceptible than either parent.
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  • 19
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 58 (1991), S. 183-189 
    ISSN: 1573-5060
    Keywords: Mycosphaerella pinodes ; pea ; Pisum sativum ; genetics ; disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In studies of the inheritance of resistance, pea seedlings of seven lines in which stems and leaves were both resistant to Mycosphaerella pinodes were crossed with a line in which they were both susceptible. With seven of the crosses resistance was dominant to susceptibility. When F2 progenies of five crosses were inoculated on either stems or leaves independently, phenotypes segregated in a ratio of 3 resistant: 1 susceptible indicating that a single dominant gene controlled resistance. F2 progenies of one other cross gave ratios with a better fit to 9 resistant: 7 susceptible indicating that two co-dominant genes controlled resistance. The F2 progeny of another cross segregated in complex ratios indicating multigene resistance. When resistant lines JI 97 and JI 1089 were crossed with a susceptible line and leaves and stems of each F2 plant were inoculated, resistance phenotypes segregated independently demonstrating that leaf and stem resistance were controlled by different genes. In two experiments where the F2 progeny of the cross JI 97×JI 1089 were tested for stem and leaf resistance separately, both characters segregated in a ratio of 15 resistant:1 susceptible indicating that these two resistant lines contain two non-allelic genes for stem resistance (designated Rmp1 and Rmp2) and two for leaf resistance (designated Rmp3 and Rmp4). Evidence that the gene for leaf resistance in JI 1089 is located in linkage group 4 of Pisum sativum is presented.
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  • 20
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The volatile selenium compound produced by cabbage (Brassica oleracea var. Capitata) when cultured on media containing either selenite or selenate is dimethyl selenide, (CH3)2 Se. The dimethyl selenide arises from enzymatic cleavage of a Se-methyl selenomethionine selenonium compound. re]19730424
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