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  • 11
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 32 (1967), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The sterol composition of several varieties of seafood was assayed by gas-liquid chromatography. Cholesterol is the major sterol (over 90%) in haddock, pollock, salmon, shrimp, and lobster. The predominant sterols in oyster are cholesterol (41%) and 24.methylenecholesterol (26%), and in crab the major sterols are cholesterol (57%) and brassicasterol (37%). Scallop and clam contain a large number of sterols, the predominant one being cholesterol (26 and 37%, respectively). The sterols other than cholesterol give different chromogens in the calorimetric analysis for sterols, which may account in part for the variability in the “cholesterol” values found in the literature.
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  • 12
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    Unknown
    Chicago : Periodicals Archive Online (PAO)
    Agricultural History. 64:4 (1990:Fall) 26 
    ISSN: 0002-1482
    Topics: History , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Journal of computational neuroscience 6 (1999), S. 215-235 
    ISSN: 1573-6873
    Keywords: hippocampus ; dentate ; calcium channels ; potassium channels ; afterpotentials ; AHP ; DAP ; burst ; calcium-dependent potassium channels ; compartmental model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Medicine , Physics
    Notes: Abstract We have constructed a detailed model of a hippocampal dentate granule (DG) cell that includes nine different channel types. Channel densities and distributions were chosen to reproduce reported physiological responses observed in normal solution and when blockers were applied. The model was used to explore the contribution of each channel type to spiking behavior with particular emphasis on the mechanisms underlying postspike events. T-type calcium current in more distal dendrites contributed prominently to the appearance of the depolarizing after-potential, and its effect was controlled by activation of BK-type calcium-dependent potassium channels. Co-activation and interaction of N-, and/or L-type calcium and AHP currents present in somatic and proximal dendritic regions contributed to the adaptive properties of the model DG cell in response to long-lasting current injection. The model was used to predict changes in channel densities that could lead to epileptogenic burst discharges and to predict the effect of altered buffering capacity on firing behavior. We conclude that the clustered spatial distributions of calcium related channels, the presence of slow delayed rectifier potassium currents in dendrites, and calcium buffering properties, together, might explain the resistance of DG cells to the development of epileptogenic burst discharges.
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Journal of computational neuroscience 8 (2000), S. 65-86 
    ISSN: 1573-6873
    Keywords: LTP ; CaM kinase II ; calmodulin ; calcium ; dendritic spine ; dentate ; computational model ; hippocampus ; phosphorylation ; autophosphorylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Medicine , Physics
    Notes: Abstract Activation of calcium/calmodulin-dependent protein kinase II (CaMKII) by calmodulin following calcium entry into the cell is important for long-term potentiation (LTP). Here a model of calmodulin binding and trapping by CaMKII in a dendritic spine was used to estimate levels and durations of CaMKII activation following LTP-inducing tetani. The calcium signal was calcium influx through NMDA receptor channels computed in a highly detailed dentate granule cell model. Calcium could bind to calmodulin and calmodulin to CaMKII. CaMKII subunits were either free, bound with calmodulin, trapped, autonomous, or capped. Strong low-frequency tetanic input produced little calmodulin trapping or CaMKII activation. Strong high-frequency tetanic input caused large numbers of CaMKII subunits to become trapped, and CaMKII was strongly activated. Calmodulin trapping and CaMKII activation were highly dependent on tetanus frequency (particularly between 10 and 100 Hz) and were highly sensitive to relatively small changes in the calcium signal. Repetition of a short high-frequency tetanus was necessary to achieve high levels of CaMKII activation. Three stages of CaMKII activation were found in the model: a short, highly activated stage; an intermediate, moderately active stage; and a long-lasting third stage, whose duration depended on dephosphorylation rates but whose decay rate was faster at low CaMKII activation levels than at high levels. It is not clear which of these three stages is most important for LTP.
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  • 15
    ISSN: 1435-0661
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: 2 and inorganic N. First-order kinetic models described the accumulation of CO2 and inorganic N and accounted for 96 to 99% of the variation in these processes. First-order rate constants (k) for net N mineralization significantly increased with temperature, but the k for microbial respiration did not increase in a consistent manner; it was 0.107 wk-1 at 5°C, 0.123 wk-1 at 10°C, and 0.101 wk-1 at 25° C. Matric potential did not significantly influence k for either process. Substrate pools for microbial respiration and net N mineralization declined between -0.01 and -0.30 Mpa, and the decline was greatest at the highest soil temperature; this response produced a significant temperature-matric potential interaction. We conclude that high rates of microbial activity at warm soil temperatures (e.g., 25°C) are limited by the diffusion of substrate to metabolically active cells. This limitation apparently lessens as physiological activity and substrate demand decline at relatively cooler soil temperature (e.g., 5°C).
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Soil Science Society of America journal 63 (1999), S. 1424-1428 
    ISSN: 1435-0661
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Acer saccharum Marshall)–red oak (Quercus rubra L.) and sugar maple–basswood (Tilia americana L.) ecosystems are Lake States forests that differ in net nitrification (5 and 15 g N m−2 yr−1, respectively), but experience equivalent rates of NO− 3 leaching following clear-cut harvest (≈5 g N m−2 yr−1). Our objectives were to determine whether high rates of N leaching are sustained following harvest and whether ecosystem-specific patterns of biomass accumulation influence NO− 3 loss. We studied two stands in each ecosystem and established four research plots in each stand; two plots were clear-cut in 1991 and two were controls. In 1996, we measured soil solution NO− 3 concentration (1-m depth) and calculated areal losses by a water balance method. We used allometric equations to estimate woody biomass in clearcut plots; herbaceous biomass was clipped. In the sugar maple–red oak ecosystem, NO− 3 leaching from 5-yr-old clear-cut plots (0.56 g N m−2 yr−1) was significantly greater than leaching from control plots (0.05 g N m−2 yr−1). In contrast, NO− 3 leaching did not differ between control (0.41 g N m−2 yr−1) and 5-yr-old clear-cut (0.02 g N m−2 yr−1) in the sugar maple–basswood ecosystem; however, loss from these clear-cut plots was significantly lower than that from clear-cut sugar maple–red oak plots. Five years after harvest, 7.1 Mg ha−1 of aboveground biomass accumulated in clear-cut sugar maple–basswood plots, almost twice that of clear-cut sugar maple–red oak plots (3.9 Mg ha−1). Five years after harvest, the highest rates of NO− 3 loss occurred in the sugar maple–red oak ecosystem, in which aboveground biomass accumulation was least.
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  • 17
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Company
    Nature biotechnology 3 (1985), S. 990-1000 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Recombinant urokinase has been refolded and purified from Escherichia coli in both its high and low molecular weight forms. The low molecular weight form of the protein has been characterized by specific activity, amino acid composition, amino terminal analysis, carboxy terminal analysis, tryptic ...
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  • 18
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] An Escherichia coli library containing cDNA sequences derived from mRNA of human pharnygeal carcinoma cells was constructed using the plasmid pBR322. The library was screened with a set of 8 deoxyoligonucleotides, 14 bases in length, which collectively code for the amino acid sequence ...
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  • 19
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 amino acids. An expression plasmid was constructed which permits ...
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  • 20
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 151 (1943), S. 531-532 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE physiology of the striated muscle of Crustacea is known to differ in several respects from that of the vertebrates; as, for example, in the peripheral location of the inhibitory process, and the presence of separate motor fibres for slow and fast contractions. The interpretation of these ...
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