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  • 11
    Electronic Resource
    Electronic Resource
    Sporophytic response to pollen selection for Alachlor tolerance in maize (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 812-817 
    Details
    ISSN: 1432-2242
    Keywords: Zea mays L. ; Pollen assay ; Gametophytic selection ; Alachlor ; Herbicide tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to assess the efficiency of male gametophytic selection (MGS) for crop improvement, pollen selection for tolerance to herbicide was applied in maize. The experiment was designed to test the parallel reactivity to Alachlor of pollen and plants grown in controlled conditions or in the field, the response to pollen selection in the sporophytic progeny, the response to a second cycle of MGS, and the transmission of the selected trait to the following generations. The results demonstrated that pollen assay can be used to predict Alachlor tolerance under field conditions and to monitor the response to selection. A positive response to selection applied to pollen in the sporophytic progeny was obtained in diverse genetic backgrounds, indicating that the technique can be generally included in standard breeding programs; the analysis of the data produced in a second selection cycle indicated that the selected trait is maintained in the next generation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01253990
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  • 12
    Electronic Resource
    Electronic Resource
    Quantitative expression of maize HSPs: genetic dissection and association with thermotolerance (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 213-220 
    Details
    ISSN: 1432-2242
    Keywords: Heat shock proteins ; Quantitative expression ; Restriction fragment length polymorphism ; Thermotolerance ; Maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In higher plants, within-species qualitative polymorphism for heat shock proteins (HSPs) is extremely rare, even between genotypes showing different heritable levels of thermotolerance. Here we have explored the amount of quantitative variability in HSP synthesis in maize. We have analyzed the quantitative expression of the typical HSPs in a set of recombinant inbreds (RIs) derived from the f1 hybrid between a thermotolerant (T232)- and a thermosensitive (CM37)-genotype, characterized for about 200 mapped RFLP loci. Significant differences were detected in the level of expression of five HSPs, and their frequency distribution in the RI population is that of a quantitative trait. Subsequent mapping of loci controlling the characters, based on RFLP analysis, confirmed the multigenic control of HSP expression: the regression analysis of the band intensities of each variant HSP on RFLPs revealed, for the different HSPs, a minimum number of three to eight quantitative trait loci (QTLs) accounting for a high proportion (0.35–0.60) of the genetic variability of these bands. An analysis of the correlation between the variability of HSPs and that of cellular membrane stability, a cellular component of thermotolerance, did not reveal any significant association of the two parameters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222081
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  • 13
    Electronic Resource
    Electronic Resource
    Haplodiploid gene expression in maize and its detection (1983)
    Springer
    Biochemical genetics 21 (1983), S. 923-931 
    Details
    ISSN: 1573-4927
    Keywords: haploid gene expression ; male gametophyte ; B-A translocations ; isozyme pattern ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A method for the demonstration of the gametophytic origin of genetic variability in maize is described. For genes coding dimeric or multimeric enzymes, haploid expression can be demonstrated by means of translocations between A and B chromosomes (TB-A), which make it possible to obtain hyperploid pollen grains, partially diploid and heterozygous for electrophoretic mobility. Comparison of the electrophoretic pattern of this pollen type (three bands) and that of normal grains produced by a heterozygous F/S plant (two bands only) reveals haploid transcription of the monomeric forms. The procedure was tested on ADH-1 and used to demonstrate haploid expression for GOT-1. The data obtained suggest, moreover, that the reduction in male gamete transmission of duplications may be due to differences in pollen competitive ability rather than to processes affecting microspore maturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00483950
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  • 14
    Electronic Resource
    Electronic Resource
    Molecular analysis and mapping of two genes encoding maize glutathione S-transferases (GST I and GST II) (1995)
    Springer
    Molecular genetics and genomics 248 (1995), S. 535-539 
    Details
    ISSN: 1617-4623
    Keywords: Glutathione S-transferase ; Maize ; Gene expression ; Mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maize glutathione S-transferase (GST) isozymes are encoded by a gene family comprising at least five genes, three of which (Gst I, II andIII) have recently been isolated and sequenced. The enzymes are active as homo or heterodimers and exhibit intraspecific polymorphism including a “null” variant for the two major isoforms expressed in roots. Northern blot analyses performed on total root RNA from “null” and “plus” genotypes, usingGst I- andGst II-specific probes, indicated that theGst I gene controls the expression of the two major GST isoforms expressed in roots.Gst I andGst II were mapped by RFLP analysis using an F2 population of 149 individuals previously characterized.Gst I was localized on the long arm of chromosome 8, while two putativeGst II loci were mapped to chromosomes 8 (70 cM fromGst I) and 10, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02423448
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