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  • Articles  (68)
  • Springer  (68)
  • 1980-1984  (52)
  • 1905-1909  (16)
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  • Articles  (68)
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  • 11
    Electronic Resource
    Electronic Resource
    Isolation of paraquat-tolerant mutants from tomato cell cultures (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 169-176 
    Details
    ISSN: 1432-2242
    Keywords: Tomato ; Cell cultures ; Herbicidetolerant mutant ; Methyl viologen ; Paraquat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tomato callus clones selected for the ability to grow at paraquat concentrations lethal to wild-type cells were found at an approximate frequency of 5×10−8 per viable cell. Diploid plants were regenerated from nine of the nineteen paraquat-tolerant callus clones isolated. Although some of these plants appeared normal, others had altered morphology and reduced vigor and fertility. New callus cultures initiated from these regenerated plants typically had at least a 30-fold increase over the wild type in tolerance to paraquat. Tests on callus from sexual progeny showed that the paraquat-tolerant phenotypes of clones PQT4, PQT6, and probably also PQT13 resulted from dominant nuclear mutations, but the number of loci involved is not yet known. Paraquat spray experiments indicated that slight paraquat-tolerance was expressed at the plant level in PQT13, but not in any of the other clones tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303704
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  • 12
    Electronic Resource
    Electronic Resource
    Assignment of the glyoxalase II gene (HAGH) to human chromosome 16 (1981)
    Springer
    Human genetics 〈Berlin〉 58 (1981), S. 358-361 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The human and rodent forms of glyoxalase II (hydroxyacylglutathione hydrolase, HAGH) can readily be separated by starch gel electrophoretic procedures. Fifty-one human-rodent somatic cell hybrid clones were examined for their human HAGH and for human enzyme markers whose genes are encoced on each autosome and the X chromosome. Sixteen clones were also examined for their human karyotypes. Human glyoxalase II segregated only with chromosome 16, demonstrating that the gene is located on this chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282815
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  • 13
    Electronic Resource
    Electronic Resource
    Human β-galactosidase and α-neuraminidase deficient mucolipidosis: Genetic complementation analysis of the neuraminidase deficiency (1982)
    Springer
    Human genetics 〈Berlin〉 60 (1982), S. 158-162 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Human β-galactosidase and α-neuraminidase deficient mucolipidosis [ML(gal-neur-)] is an inherited lysosomal enzymopathy which recently was designated as a sialidosis. We analyzed the neuraminidase deficiency of this disorder with genetic complementation analyses using a heterokaryon enrichment procedure. The genetic defects of two apparent variants of this disorder complemented the defects of the neuraminidase deficiency diseases, sialidosis I and mucolipidosis I, resulting in the restoration of neuraminidase activity in heterokaryons. The neuraminidase deficiency, therefore, may not be the primary defect in ML(gal-neur-) and is not an appropriate test for determining carrier status. The clinical and biochemical characteristics of this disorder suggest that a post-translational or processing event for these enzymes may be defective. The defect, however, is different from I-cell disease and pseudo-Hurler polydystrophy, two disorders of post-translational lysosomal enzyme biosynthesis, since complementation studies demonstrated recovery of intracellular β-galactosidase and α-neuraminidase levels in heterokaryons. The lack of human β-galactosidase expression in man-mouse somatic cell hybrids formed from fibroblasts of the infantile onset type disorder suggests that the defect is not corrected by the mouse genome. The ML(gal-neur-) disorder therefore appears to be a distinct subtype of the inherited neuraminidase deficiencies in which the defect may occur in a post-translational or regulatory step which coordinately affects the expression of lysosomal β-galactosidase and α-neuraminidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569704
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  • 14
    Electronic Resource
    Electronic Resource
    Discrimination between the red- and far-red-absorbing forms of phytochrome from Avena sativa L. by monoclonal antibodies (1984)
    Springer
    Planta 160 (1984), S. 382-384 
    Details
    ISSN: 1432-2048
    Keywords: Avena (phytochrome) ; Immunological discrimination ; Monoclonal antibody ; Phytochrome (red-, far-red absorbing forms)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A set of rat monoclonal antibodies (ARC MAC 48 to 52 and 54 to 56), raised to phytochrome from dark-grown seedlings of Avena sativa L. was tested for the ability to discriminate between the red-absorbing (Pr) and far-red-absorbing (Pfr) forms of phytochrome by indirect enzyme-linked immunosorbent assay. MAC 50 bound more strongly to Pfr and MAC 49 and 52 showed preferential binding to Pr from extracts of dark-grown Avena seedlings; MAC 50 also bound more strongly to Pfr from brushite-purified phytochrome. The remainder of the monoclonal antibodies and a rabbit polyclonal antiphytochrome preparation did not discriminate between Pr and Pfr. The results provide evidence for conformational changes in defined regions of the phytochrome apoprotein upon photoconversion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393420
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  • 15
    Electronic Resource
    Electronic Resource
    Regulation of swelling of etiolated-wheat-leaf protoplasts by phytochrome and gibberellic acid (1983)
    Springer
    Planta 158 (1983), S. 416-421 
    Details
    ISSN: 1432-2048
    Keywords: Gibberellin and protoplast swelling ; Phytochrome ; Protoplast (swelling) ; Triticum (protoplast swelling)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of light on the size of intact protoplasts isolated from the primary leaves of etiolated Triticum aestivum was studied. A 2-min red-light irradiation in the presence of 1 mM KCl was sufficient to cause a swelling of protoplasts compared with those maintained in darkness. The effect was photoreversible by far-red light over two light cycles, indicating the involvement of phytochrome. At 4°C, escape from reversibility occurred between 2 and 5 min after the exposure to red light. In exposure-response experiments, 20 s red light at 27 μmol m-2s-1 was sufficient to saturate the response. Exogenous gibberellic acid added in darkness in the presence of KCl also induced protoplast swelling. Gibberellins may act as an intermediate in the phytochrome-induced swelling of protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397734
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  • 16
    Electronic Resource
    Electronic Resource
    Efficient hybridization between Lycopersicon esculentum and L. peruvianum via embryo callus (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 215-219 
    Details
    ISSN: 1432-2242
    Keywords: Interspecific hybridization ; Embryo callus ; Plant regeneration ; Lycopersicon esculentum ; Lycopersicon peruvianum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Embryo callus was produced in up to 12% of the undeveloped seeds of the interspecific cross L. esculentum cv. VFNT cherry x L. peruvianum LA1283-4, a cross which does not produce viable seeds. Plants were produced from 90% of those callus clones by regeneration and rooting. Evidence that these plants were hybrid includes morphology, isoenzyme patterns and fertility relations. Both diploid and tetraploid plants were produced, 40% of the regenerated callus clones producing at least one diploid plant. Thus, up to 4% of the undeveloped seeds plated for callus production eventually yielded diploid hybrid plants. In contrast, among 401 undeveloped seeds dissected, no embryos suitable for embryo culture were found. A backcross of the embryo callus hybrids to the L. esculentum parent has succeeded — producing, as expected, only undeveloped seeds; these undeveloped seeds have produced callus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265495
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  • 17
    Electronic Resource
    Electronic Resource
    Glutathione-S-transferase gene mapped to chromosome 11 is GST3 not GST1 letter (1984)
    Springer
    Somatic cell and molecular genetics 10 (1984), S. 319-320 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01535254
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  • 18
    Electronic Resource
    Electronic Resource
    Coronavirus 229E susceptibility in man-mouse hybrids is located on human chromosome 15 (1982)
    Springer
    Somatic cell and molecular genetics 8 (1982), S. 83-94 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Human coronavirus 229E, an enveloped, RNA-containing virus, causes respiratory illness in man and is serologically related to murine coronavirus JHM, which causes acute and chronic demyelination in rodents. 229E displays a species-specific host range restriction whose genetic basis was studied in human-mouse hybrids. 229E replicated in human WI-38 cells but not in three mouse cell lines tested (RAG, LM/TK−, and A9). Human coronavirus sensitivity (HCVS) was expressed as a dominant phenotype in hybrids, indicating that mouse cells do not actively suppress 229E replication. HCVS segregated concordantly with the human chromosome 15 enzyme markers mannose phosphate isomerase (MPI) and the muscle form of pyruvate kinase (PKM2), and analysis of hybrids containing an X/15 translocation [t(X;15)(p11;q11)] localized HCVS to the q11 → qter region of chromosome 15. HCVS might code for a specific surface receptor, allowing 229E to be absorbed to and received within the host cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538652
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  • 19
    Electronic Resource
    Electronic Resource
    A simple method for obtaining cultures enriched for Type II pneumonocytes from fetal rabbit (1982)
    Springer
    Methods in cell science 7 (1982), S. 63-68 
    Details
    ISSN: 1573-0603
    Keywords: fetal lung ; Type II cells ; culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A differential plating method permitted preparation of cultures significantly enriched for Type II pneumocytes. These cells were maintained in a differentiated state for at least 12 d, identifiable morphologically (by presence of osmiophilic lamellar inclusion bodies) and bio-chemically (by demonstration of synthesis of phosphatidyl choline and production of disaturated lecithin).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01665911
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  • 20
    Electronic Resource
    Electronic Resource
    Establishment of epithelial cell lines from individual rabbit lenses (1980)
    Springer
    Methods in cell science 6 (1980), S. 57-60 
    Details
    ISSN: 1573-0603
    Keywords: rabbit ; lens epithelium ; primary culture ; cell line ; lens crystallins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We describe a method of initiating primary cell cultures from lenses of rabbits by an explant-outgrowth technique. These primary cultures routinely yield epithelial cell lines when subcultured with collagenase-trypsin chicken serum. Most lines examined retain the diploid chromosome number through at least 30 passages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01666017
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