ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The crystal structure of the alternating RNA r(CGUAC)dG with a 3′-terminal deoxy G residue has been determined at 1.76 Å resolution. The crystal belongs to the orthorhombic space group C2221, unit-cell dimensions a = 29.53, b = 44.61 and c = 94.18 Å, with two independent duplexes (I and II) per asymmetric unit. The structure was solved by the molecular-replacement method. The final R factor was 18.8% using 4757 reflections in the resolution range 8.0–1.76 Å. The model contains a total of 496 atoms and 85 solvent molecules. The two duplexes form the repeating unit and stack in the usual head-to-tail (5′,3′/5′,3′) fashion into a pseudocontinuous helical column. Almost all of the 2′-hydroxyl groups are engaged in the three modes of water-mediated interactions to the base N3/O2 atoms, the sugar O4′ atoms and the backbone phosphates. Thus, the 2′-hydroxyl group of RNA is probably contributing to the stability of the duplexes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444997013097
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