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  • 11
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Water Resources Association 32 (1996), S. 0 
    ISSN: 1752-1688
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: : Determination of the boundary conditions for modeling ground water flow is a critical point especially in regional models. Normally the regional models require model areas that are greater than the given area of interest. This work focuses on the prediction of hydraulic heads in regional models using flux boundary conditions. The model uses flux boundary conditions that were estimated using a radial flow analog and Darcy's law. The regional model that is presented uses no parameter identification (inverse estimation) procedures. In the present work, the Houston area was used. The simulation of the hydrological conditions of the Chicot and Evangeline Aquifers that underlie the Houston area were made using the available information about the geological profile in the Houston region and the current information about the existing production wells. The regional model works as a forward problem. The system parameters such as hydraulic conductivity, specific storage, and hydrological stresses were specified, and the model predicts the hydraulic head. Actual data from piezometers operated by the U.S. Geological Survey (USGS) in many places throughout Houston were used as initial conditions. Some piezometric head data were generated using the regional variable theory called kriging to supply head estimates in areas where data were unavailable. The Modular Three Dimensional Finite Difference Groundwater Flow Model developed by the USGS was used to predict the hydraulic heads. The predicted ground water heads are compared to the actual data. The results show that the model performs well for locations where data were available.
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  • 12
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Water Resources Association 30 (1994), S. 0 
    ISSN: 1752-1688
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: : The proportionality coefficient, K, and the weighing parameter, X, required for the Muskingum-Cunge Flood Routing Method are dependent on the hydraulic characteristics of the channel and the dynamic characteristic of the flood wave. This work focuses on the determination of the Muskingum-Cunge Flood Routing Method parameters for streams where measured hydrographs are not available (i.e., ungaged streams) with floods that stay within the channel banks. In the present work, a gaged creek was used and a dynamic wave was routed to test the reliability of the parameters determined through the Schaefer and Stevens technique (Schaefer and Stevens, 1978). The predicted outflow hydrographs are compared to the hydrographs obtained for the same stream determined with the Muskingum Routing option of the HEC-1 program. Cypress Creek in Harris County, Texas, was the model for this work; and the corresponding data were extracted from the Grant Road and Westfield, Texas, USGS gaging stations.
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  • 13
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 67 (2002), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : Inulin, sucrose, fructose, and glucose contents in tubers of Helianthus tuberosus (Jerusalem artichoke) and Cichorium intybus stored at different temperatures (-18, 4, and 18 °C) after harvesting were followed. Inulin content in both tubers decreased during storage. In C. intybus this decrease was associated with increases in glucose and fructose contents. In H. tuberosus the fructan fraction having molecular weight between 800 and 1200 increased after sucrose reached its maximum content [1.3–10−1 g(g d.w.)−1] in tubers stored at 4 °C. Fructose-to-glucose ratio was followed in H. tuberosus tubers harvested at different times; samples from plants subjected to different fertilization treatments were used. This parameter can be used for choosing the harvest date since it is related to the disappearance of the fructan fraction having molecular weight higher than 1200.
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  • 14
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 59 (1994), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: One high-fat and four low-fat pork sausage formulations were made: P30 with 30% pork fat (P); P11-W with 11% P and 10% added water (W); P11-WC with 11% P, 9.5% W and 0.5% carrageenan (C); Pso11-WC with 6% P plus 5% high-oleic sunflower oil (So), 9.5% W and 0.5% C; and Pso11-WGcf with 6% P plus 5% So, 7.0% W and 3% defatted glandless cottonseed flour (Gcf). Sausage patty cooking yield was highest for P11-WC and Pso11-WGcf. TBA values for refrigerated precooked patties were lowest for Pso11-WGcf, whereas carrageenan had a prooxidant effect in low-fat patties made with all-pork fat. The oil had no adverse effects on lipid oxidation and sensory properties of low-fat patties with C. The monounsaturated to saturated fatty acid ratio increased by 53–71% in the So-containing low-fat patties when compared to P11-W and P11-WC patties.
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  • 15
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Ground beef with 26.3% fat was cooked by browning/draining (BD), browning/rinsing (BR), and an oil-extraction method with (OA) or without (ON) meat broth added back. BR caused the most reduction of total fat and cholesterol, whereas ON and OA yielded less cooked meat than BD and BR. ON and OA cooked samples, compared to BD and BR samples, contained more polyunsaturated fatty acids and nonheme iron and had higher TBA values unon extended storage at 4°C. Trained sensory panelists rated BD or BR cooked ground beef more intense in positive flavor note (“cooked beef/brothy”) than ON or OA meat. Likewise, consumers tended to prefer meatloaf, spaghetti sauce, and chili prepared with BD or BR cooked ground beef to those prepared with ON or OA cooked meat.
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  • 16
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 58 (1993), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In a modified method for measuring Resistant Starch (RS) in dietary fiber residues all operations for obtaining fiber residues and determination of RS were performed in a 50 mL centrifugation tube. This minimized error sources and simplified previous methodology.
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  • 17
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 30 (1992), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: A method is presented to estimate elastic and inelastic specific storage and vertical hydraulic conductivities in aquitards in multilayered flow systems. Borehole extensometer records and ground-water hydrographs from piezometers are used to construct plots of effective stress and deformation. Elastic and inelastic specific storage are estimated from the plots of effective stress and deformation during loading and unloading cycles. The elapsed loading time is estimated from the same plots and is used to calculate vertical hydraulic conductivity using Terzaghi's consolidation theory. The method is applied to three sites in Houston, Texas.
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  • 18
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The physiology and central metabolism of a ppc mutant Escherichia coli were investigated based on the metabolic flux distribution obtained by 13C-labelling experiments using gas chromatography–mass spectrometry (GC–MS) and 2-dimensional nuclear magnetic resonance (2D NMR) strategies together with enzyme activity assays and intracellular metabolite concentration measurements. Compared to the wild type, its ppc mutant excreted little acetate and produced less carbon dioxide at the expense of a slower growth rate and a lower glucose uptake rate. Consequently, an improvement of the biomass yield on glucose was observed in the ppc mutant. Enzyme activity measurements revealed that isocitrate lyase activity increased by more than 3-fold in the ppc mutant. Some TCA cycle enzymes such as citrate synthase, aconitase and malate dehydrogenase were also upregulated, but enzymes of glycolysis and the pentose phosphate pathway were downregulated. The intracellular intermediates in the glycolysis and the pentose phosphate pathway, therefore, accumulated, while acetyl coenzyme A and oxaloacetate concentrations decreased in the ppc mutant. The intracellular metabolic flux analysis uncovered that deletion of ppc resulted in the appearance of the glyoxylate shunt, with 18.9% of the carbon flux being channeled via the glyoxylate shunt. However, the flux of the pentose phosphate pathway significantly decreased in the ppc mutant.
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  • 19
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The metabolic regulation of Escherichia coli lacking a functional pykF gene was investigated based on gene expressions, enzyme activities, intracellular metabolite concentrations and the metabolic flux distribution obtained based on 13C-labeling experiments. RT-PCR revealed that the glycolytic genes such as glk, pgi, pfkA and tpiA were down regulated, that ppc, pckA, maeB and mdh genes were strongly up-regulated, and that the oxidative pentose phosphate pathway genes such as zwf and gnd were significantly up-regulated in the pykF mutant. The catabolite repressor/activator gene fruR was up-regulated in the pykF mutant, but the adenylate cyclase gene cyaA was down-regulated indicating a decreased rate of glucose uptake. This was also ascertained by the degradation of ptsG mRNA, the gene for which was down-regulated in the pykF mutant. In general, the changes in enzyme activities more or less correlated with ratios of gene expression, while the changes in metabolic fluxes did not correlate with enzyme activities. For example, high flux ratios were obtained through the oxidative pentose phosphate pathway due to an increased concentration of glucose-6-phosphate rather than to favorable enzyme activity ratios. In contrast, due to decreased availability of pyruvate (and acetyl coenzyme A) in the pykF mutant compared with the wild type, low flux ratios were found through lactate and acetate forming pathways.
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  • 20
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Based on the observation of large conductance states formed by Bacillus thuringiensis Cry toxins in synthetic planar lipid bilayers and the estimation of a pore size of 10–20 Å, it has been proposed that the pore could be formed by an oligomer containing four to six Cry toxin monomers. However, there is a lack of information regarding the insertion of Cry toxins into the membrane and oligomer formation. Here we provide direct evidence showing that the intermolecular interaction between Cry1Ab toxin monomers is a necessary step for pore formation and toxicity. Two Cry1Ab mutant proteins affected in different steps of their mode of action (F371A in receptor binding and H168F in pore formation) were affected in toxicity against Manduca sexta larvae. Binding analysis showed that F371A protein bound more efficiently to M. sexta brush border membrane vesicles when mixed with H168F in a one to one ratio. These mutant proteins also recovered pore-formation activity, measured with a fluorescent dye with isolated brush border membrane vesicles, and toxicity against M. sexta larvae when mixed, showing that monomers affected in different steps of their mode of action can form functional hetero-oligomers.
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