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  • Springer  (143)
  • 1995-1999  (121)
  • 1975-1979  (22)
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 33 (1977), S. 737-738 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Combined effect of veratridine and sodium aspartate on rabbit retinas in vitro was studied. Observations suggested that mode of action of veratridine on the PII and PIII components of ERGs was modified by the presence of sodium aspartate in perfusate.
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  • 12
    ISSN: 1432-1432
    Keywords: Key words: Cyanobacteria —Synechococcus— Phylogeny — Ribosomal RNA — Maximum-likelihood method — Neighbor-joining method — Rate homo- and heterogeneous models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Although molecular phylogenetic studies of cyanobacteria on the basis of the 16S rRNA gene sequence have been reported, the topologies were unstable, especially in the inner branchings. Our analysis of 16S rRNA gene phylogeny by the maximum-likelihood and neighbor-joining methods combined with rate homogeneous and heterogeneous models revealed seven major evolutionary lineages of the cyanobacteria, including prochlorophycean organisms. These seven lineages are always stable on any combination of these methods and models, fundamentally corresponding to phylogenetic relationships based on other genes, e.g., psbA, rbcL, rnpB, rpoC, and tufA. Moreover, although known genotypic and phenotypic characters sometimes appear paralleled in independent lineages, many characters are not contradictory within each group. Therefore we propose seven evolutionary groups as a working hypothesis for successive taxonomic reconstruction. New 16S rRNA sequences of five unicellular cyanobacterial strains, PCC 7001, PCC 7003, PCC 73109, PCC 7117, and PCC 7335 of Synechococcus sp., were determined in this study. Although all these strains have been assigned to ``marine clusters B and C,'' they were separated into three lineages. This suggests that the organisms classified in the genus Synechococcus evolved diversely and should be reclassified in several independent taxonomic units. Moreover, Synechococcus strains and filamentous cyanobacteria make a monophyletic group supported by a comparatively high statistical confidence value (80 to 100%) in each of the two independent lineages; therefore, these monophylies probably reflect the convergent evolution of a multicellular organization.
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Higher-order and symbolic computation 9 (1996), S. 243-277 
    ISSN: 1573-0557
    Keywords: Real-time computing ; computational reflection ; meta-level programming ; object-based concurrency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract In this paper we present an application of computational reflection in the programming oftime-dependent systems. A time-dependent system performs its tasks according to timing specifications specified within the system or imposed from outside the system. Reflective techniques can be applied to programming time-dependent systems because (1) some application programs require the introduction of a new language construct for specifying timing requirements and (2) different applications may require domain-specific scheduling algorithms. To allow a programmer to add or modify language constructs or scheduling algorithms, however, a clear reflective architecture and program interfaces must be provided. This paper proposes a concurrent object-based reflective architecture (R 2 architecture) for time-dependent computing. This architecture is based on anindividual reflection scheme and introduces new meta-level objects (real-time meta objects) that are responsible for time-dependent capabilities. An alarm-clock object and a scheduler object are introduced, and message protocols between them and real-time meta objects are defined. We implemented this architecture on ABCL/R2 and created the Sampled Sound Player program as an application. With this application we provided three different scheduler objects and measured the impact of different scheduling algorithms on sound playback. The measured results show that a scheduler with more complex computations at the meta level exhibited less scheduling overhead, thus was capable of better sound playback. The other example, Time-dependent Graceful Degradation Scheme, demonstrates the programming of functionality degradation triggered by failure to satisfy timing specifications.
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Journal of statistical physics 85 (1996), S. 681-743 
    ISSN: 1572-9613
    Keywords: Diffusion-limited aggregation ; pattern formation ; branched growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A recently proposed theory for diffusion-limited aggregation (DLA), which models this system as a random branched growth process, is reviewed. Like DLA, this process is stochastic, and ensemble averaging is needed in order to define multifractal dimensions. In an earlier work by Halsey and Leibig, annealed average dimensions were computed for this model. In this paper, we compute the quenched average dimensions, which are expected to apply to typical members of the ensemble. We develop a perturbative expansion for the average of the logarithm of the multifractal partition function; the leading and subleading divergent terms in this expansion are then resummed to all orders. The result is that in the limit where the number of particlesn→∞, the quenched and annealed dimensions areidentical; however, the attainment of this limit requires enormous values ofn. At smaller, more realistic values ofn, the apparent quenched dimensions differ from the annealed dimensions. We interpret these results to mean that while multifractality as an ensemble property of random branched growth (and hence of DLA) is quite robust, it subtly fails for typical members of the ensemble.
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 49 (1998), S. 72-76 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Culture conditions of Schizochytrium limacinum SR21 for the purpose of microbial docosahexaenoic acid (DHA) production were investigated. The strain SR21 showed a wide tolerance to salinity; that is, the optimum salinity was between 50% and 200% that of sea water. Monosaccharides (glucose and fructose) and glycerol supported good cell growth and DHA yield. Di- and polysaccharides, oleic acid, and linseed oil gave low DHA yields. A high content of DHA (more than 30% of total fatty acids) was obtained from culture on glucose, fructose, and glycerol, and also the strain had simple polyunsaturated fatty acid profiles. The major polyunsaturated fatty acids other than DHA were n-6 docosapentaenoic acid only, and the contents of icosapentaenoic acid and arachidonic acid were less than 1%. Using corn steep liquor as a nitrogen source, a high total fatty acid content was obtained. The total fatty acid content in the dry cell weight increased as the concentration of the nitrogen source decreased, reached more than 50%. An increase in carbon source concentration led to a high DHA yield. A maximum DHA yield of more than 4 g/l was obtained in both glucose and glycerol media at 9% and 12% respectively. S. limacinum SR21 was thought to be a promising resource for microbial DHA production yielding a good level of productivity as well as a simple polyunsaturated fatty acid profile.
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  • 16
    ISSN: 1432-0592
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Geography , Economics
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 52 (1996), S. 691-694 
    ISSN: 1420-9071
    Keywords: Circadian rhythm ; intracerebroventricular infusion ; light pulse ; phase shift ; rats ; vitamin B12
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Vitamin B12 (VB12) is a putative modulator of the human circadian clock, improving entrainability to the 24 h light-dark cycle. The present study was intended to elucidate the mechanism of VB12 action in an animal model. In male rats free-running under constant dim illumination, a single light pulse of 50–1000 lux for 20 min given at circadian time (CT) 20 induced a 0.28 to 1.08 h phase advance and at CT 14 induced a 0.54 to 2.10 h phase delay. A 3 h intracerebroventricular (icv) infusion of 30 nmol VB12 starting 2 h prior to a 20 min 200 lux light pulse significantly amplified phase shifts in comparison with saline-treated or untreated controls. The mean phase advance (1.13 h) was 1.8-fold greater than that of saline-infused controls, whereas the mean phase delay (2.28 h) was 2.9-fold greater. These values were comparable to the maximal phase shifts caused by 1000 lux light pulses in untreated rats. Since the same VB12 treatment alone had failed to induce a phase shift in a previous experiment, these results indicate that VB12 strongly enhanced light pulse-induced phase shifts and thus augmented the entrainability of the circadian clock to light.
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Optical review 2 (1995), S. 163-166 
    ISSN: 1349-9432
    Keywords: nonlinear filters ; image processing ; filter design method ; learning filter optimization ; mathematical morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A simulator to aid in the design of nonlinear image processing filters is developed. This simulator assists the filter design by determining parameters manually by trial-and-error. The method is also useful for improving the results of the learning optimization methods. An example of the application of the technique to improve a resultant filter by a learning method of a mathematical morphological filter is shown.
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  • 19
    ISSN: 1432-0827
    Keywords: Key words: Bone morphogenetic protein — Osteogenic protein-1 — Transforming growth factor-β— Osteoblasts — Growth factors.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Bone morphogenetic proteins (BMPs) are novel growth and differentiation factors that act on mesenchymal stem cells to initiate new bone formation in vivo and promote the growth and differentiation of cells in the osteoblastic lineage. In the present study, we examined the effects of recombinant human osteogenic protein-1 (also known as BMP-7) on the expression of related members of the BMP family using SaOS-2 and U2-OS, two human osteosarcoma cell strains. Evaluation of BMP-2, -4, and -6 mRNA expression indicates that OP-1 stimulated the mRNA levels of BMP-6 in both SaOS-2 cells (threefold) and U2-OS cells (fivefold) after 24 hours of treatment, while decreasing the mRNA levels of BMP-4 in SaOS-2 cells (80%) and BMP-2 and BMP-4 in U2-OS cells by 50% and 72%, respectively. BMP-2 mRNA expression, as examined by Northern blot analysis, was below detectable limits in SaOS-2 cultures. These results demonstrate that OP-1 modulates the mRNA expression of related members of the BMP family, suggesting a possible mode of action of OP-1 on the growth and differentiation of cells in the osteoblastic lineage in vitro.
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  • 20
    ISSN: 1432-0827
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract The skeletal contents of insulin-like growth factor-2 (IGF-II), insulin-like growth factor binding protein-5 (IGFBP-5), and insulin-like growth factor binding protein-3 (IGFBP-3) were determined in duplicate samples of human femoral cortical bone obtained from 64 subjects (44 males and 20 females) between the ages of 20 and 64 years. The results of these quantitative measurements revealed an age-related decrease in the femoral cortical content of IGFBP-5 (r=-0.272, P=0.031) in the total population. Although the femoral cortical content of IGF-II did not show a similar decrease with age, it could be correlated to the femoral cortical content of IGFBP-5 (r=0.442, P〈0.001). In constrast, the femoral cortical content of IGFBP-3 did not decrease with age and could not be correlated to the femoral cortical contents of either IGFBP-5 or IGF-II. Comparisons of these results with previous measurements of insulin-like growth factor-1 (IGF-I) and transforming growth factor-β (TGF-β), in extracts of the same bones, showed significant cross-correlations between the femoral cortical contents of each of these growth factors and the femoral cortical contents of IGFBP-5 (r=0.625 for IGF-I versus IGFBP-5, r=0.554 for TGF-β versus IGFBP-5, P〈0.001 for each) but not IGFBP-3. Together, these data indicate average net losses of 60% and 29% of the femoral cortical contents of IGF-I and IGFBP-5, respectively, and apparent net losses (i.e., nonsignificant decreases) of 21% and 25% of the femoral cortical contents of IGF-II and TGF-β, respectively, between the third and the sixth decades (i.e., decreases from young adult values of 75.1 pmol/g of bone for IGF-I, 124.7 pmol/g of bone for IGF-II, 0.71 pmol/g of bone for TGF-β, 115.6 pmol/g of bone for IGFBP-5, and 26.2 pmol/g of bone for IGFBP-3).
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