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  • 11
    Electronic Resource
    Electronic Resource
    Effect of the carbohydrate moiety on the secondary structure of .beta.2-glycoprotein. I. Implications for the biosynthesis and folding of glycoproteins (1990)
    s.l. : American Chemical Society
    Biochemistry 29 (1990), S. 6250-6257 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00478a020
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  • 12
    Electronic Resource
    Electronic Resource
    Structure of the N- and O-glycans of the A-chain of human plasma .alpha.2HS-glycoprotein as deduced from the chemical compositions of the derivatives prepared by stepwise degradation with exoglycosidases (1992)
    s.l. : American Chemical Society
    Biochemistry 31 (1992), S. 12198-12203 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00163a032
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  • 13
    Electronic Resource
    Electronic Resource
    The sugar-specific outer membrane channel ScrY contains functional characteristics of general diffusion pores and substrate-specific porins (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Escherichia coli K–12 strain PS1–28–37 carries the multicopy plasmid pPSO28–37 containing a DNA fragment coding for two of the proteins that enable bacteria to utilize sucrose as sole carbon source. One of the different gene products of the plasmid is the outer membrane protein, ScrY. This protein was isolated and purified by chromatography across a gel filtration column. Reconstitution experiments with lipid by layer membrane demonstrated that ScrY formed ion-permeable channels with properties very similar to those of general diffusion pores of enteric bacteria. The presence of sugars in the aqueous phase led to a dose-dependent block of ion transport through the channel, like the situation found with LamB (maltoporin) of Escherichia coli and Salmonella typhimurium. The binding constants of a variety of different sugars were determined. The stability constant for malto-oligosaccharide binding increased with increasing numbers of glucose residues. Disaccharides generally had a larger binding constant than monosaccharides. The binding of different sugars to ScrY and LamB of E. coli discussed with respect to the kinetics of sugar movement through the channel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb02153.x
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  • 14
    Electronic Resource
    Electronic Resource
    Molecular analysis of two fructokinases involved in sucrose metabolism of enteric bacteria (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Sucrose-positive derivatives of Escherichia coli K-12, containing the plasmid pUR400, and of Klebsiella pneumoniae hydrolyse intracellular sucrose 6-phosphate by means of an invertase into d-glucose 6-phosphate and free d-fructose. The latter is phosphorylated by an ATP-dependent fructokinase (gene scrK of an scr regulon) to d-fructose 6-phosphate. The lack of ScrK does not cause any visible phenotype in wild-type strains of both organisms. Using genes and enzymes normally involved in d-arabinitol metabolism from E. coli C and K. pneumoniae, derivatives of E. coli K-12 were constructed which allowed the identification of scrK mutations on conventional indicator plates. Cloning and sequencing of scrK from sucrose plasmid pUR400 and from the chromosome of K. pneumoniae revealed an open reading frame of 924 bp in both cases — the equivalent of a peptide containing 307 amino acid residues (Mr, 39 and 34 kDa, respectively, on sodium dodecyl sulphate gels). The sequences showed overall identity among each other (69% identical residues) and to a kinase from Vibrio alginoiyticus (57%) also involved in sucrose metabolism, lower overall identity (39%) to a d-ribose-kinase from E. coli, and local similarity to prokaryotic, and eukaryotic phosphofructokinases at the putative ATP-binding sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb01851.x
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  • 15
    Electronic Resource
    Electronic Resource
    A sugar-specific porin, ScrY, is involved in sucrose uptake in enteric bacteria (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: During the molecular analysis of a plasmid-coded sucrose metabolic pathway of enteric bacteria, a gene, scrY, was found whose product, ScrY, had all the properties of a bacterial porin (Schmid et al, 1988). Loss of this protein (Mr 58kDa), localized in the outer membrane, led, as shown here, to an increase in the apparent Km for sucrose transport in whole cells from 10 μM in wild-type cells to 300 μM in mutant cells. This contrasts with the Km for sucrose phosphorylation as measured in membrane vesicles from mutant and wild-type cells, which remained unchanged at about 10 μM, and reflects the activity of the sucrose-specific Enzymell of the phosphoenolpyruvate-dependent carbohydrate:phosphotransferase system (PTS) responsible for uptake through the inner membrane. Furthermore, the presence of ScrY restored growth on maltodextrins in cells devoid of LamB, thus complementing the lack of this maltoporin. The amino acid sequence deduced from the DNA sequence was determined for the plasmid-coded and the ScrY porin coded in the chromosome of Klebsiella pneumoniae. Both show high identity (86%) to each other, and to the channel domain of LamB, further corroborating the conclusion that they constitute porins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb00769.x
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  • 16
    Electronic Resource
    Electronic Resource
    Restricted range of ocular accommodation in barn owls (Aves:Tytonidae) (1991)
    Springer
    Journal of comparative physiology 168 (1991), S. 299-303 
    Details
    ISSN: 1432-1351
    Keywords: Photorefraction ; Accommodation ; Tytonidae ; Near vision
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In an examination of the focusing abilities of 15 species of owls, the North American barn owl, Tyto alba pratincola (Bonaparte 1838), was an outstanding accommodator, having a range of accommodation exceeding 10 diopters (Murphy and Howland 1983). Using comparable methods, we examined the accommodation of 4 specimens of the Australian barn owl, Tyto alba delicatula (Gould 1837). We failed to elicit accommodation greater than two diopters, and most stimuli failed to evoke any discernable accommodation at all. Furthermore, examination of other Australian tytonid owls, the grass owl, T. longimembris, the sooty owl, T. tenebricosa, and both the mainland and Tasmanian subspecies of the masked owl, T. novaehollandiae novaehollandiae and T. novaehollandiae castanops, also failed to reveal anything but very moderate accommodative ranges. We conclude that the outstanding accommodative ability of the American barn owl is truly an exception to the modest accommodative abilities of the tytonid owls generally.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198349
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  • 17
    Electronic Resource
    Electronic Resource
    Focusing and accommodation in tuatara (Sphenodon punctatus) (1992)
    Springer
    Journal of comparative physiology 170 (1992), S. 263-266 
    Details
    ISSN: 1432-1351
    Keywords: Tuatara ; Sphenodon punctatus ; Accommodation ; Photorefraction ; Corneal curvature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Photorefraction and photokeratometry were performed on two juvenile tuatara (Sphenodon punctatus, 7 years of age, total length approx. 27 cm). Sphenodon is the only surviving genus of the Rhynchocephalia, an order of reptiles. Both existing species are endangered and are found only in New Zealand. Even though Walls (1942) has stated that the ciliary muscle is relatively weak in tuataras, we observed 8 D of accommodation. The eyes were found to focus independently and we could induce an anisometropia of 6 D. The average corneal power of the tuatara was found to be 101 D.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00191413
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  • 18
    Electronic Resource
    Electronic Resource
    Focusing and accommodation in the brown kiwi (Apteryx australis) (1992)
    Springer
    Journal of comparative physiology 170 (1992), S. 687-689 
    Details
    ISSN: 1432-1351
    Keywords: Brown kiwi ; Apteryx australis ; Accommodation ; Photorefraction ; Corneal curvature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Brown kiwis are an endangered species of nocturnal, flightless birds which are native to New Zealand. The resting focus of two specimens has previously been studied by retinoscopy in a zoo while the birds were restrained by their keeper (Sivak and Howland 1987). Those birds appeared to be hyperopic (farsighted) by 2–7 D. In this study, examination with infrared photorefraction of the focusing of two unrestrained, feeding birds showed that they could focus objects at infinity and objects in their immediate environment and that they had modest powers of accommodation. Measurements on two 6 month old kiwi chicks showed their corneal radius of curvature to be between 2.90 and 3.00 mm (117 D and 101 D in power).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198978
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  • 19
    Electronic Resource
    Electronic Resource
    Undegraded chromogranin A is present in serum and enters the endocytotic lysosomal pathway in kidney (1991)
    Springer
    Histochemistry and cell biology 96 (1991), S. 395-399 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Analysis of human and bovine serum by immunoblotting revealed the presence of the proprotein chromogranin A. By the same method chromogranin A was also found in rat, bovine and human kidney. However this organ did not contain any chromogranin A mRNA arguing against a synthesis within this organ. By immun-electron microscopy chromogranin A immunoreactivity was found in proximal tubule cells of rat kidney. Positive immunostaining was present in small vesicles within and in close proximity to the brush border and closer to the nucleus in typical lysosomal structures. These results make it likely that chromogranin A from serum reaches kidney tubule cells by glomerular filtration and is taken up into the endocytotic lysosomal pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315996
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  • 20
    Electronic Resource
    Electronic Resource
    On the treatment of the center of mass motion in nuclear mean field theories (1990)
    Springer
    The European physical journal 336 (1990), S. 5-26 
    Details
    ISSN: 1434-601X
    Keywords: 21.60.Cs ; 21.60.Jz
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract It is shown how the spurious components due to the center of mass motion can be eliminated from general Hartree-Fock-Bogoliubov quasi-particle configurations with the help of projection techniques. The problem how to restore the additional symmetries being broken by such configurations is discussed. An explicit formulation is given for the spherical Hartree-Fock problem with center of mass momentum projection before the variation. As an example for the application of this method the ground state of4He is studied using two different interactions, a microscopic two-body one as well as a phenomenological one including a Skyrme-type three-body force. The results are compared to those of the usual approximate treatment of the center of mass motion in Hartree-Fock calculations. It turns out that, at least for the chosen example, the latter yields a rather reasonable approximation to the correct total energy, single particle energy and even the mass density provided that it is calculated from a translationally invariant density operator.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01904157
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