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  • 2010-2014  (67)
  • 1995-1999  (111)
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  • 11
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    Nanoparticle-Mediated Gene Transfer From Electrophoretically Coated Metal Surfaces (2012)
    American Chemical Society (ACS)
    Details
    Publication Date: 2012-07-14
    Description: The Journal of Physical Chemistry B DOI: 10.1021/jp303448v
    Electronic ISSN: 1520-5207
    Topics: Chemistry and Pharmacology , Physics
    Published by American Chemical Society (ACS)
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  • 12
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    Remote Sensing, Vol. 3, Pages 2440-2460: An Object-Based Classification of Mangroves Using a Hybrid Decision Tree—Support Vector Machine Approach (2011)
    MDPI Publishing
    Details
    Publication Date: 2011-11-18
    Description: Mangroves provide valuable ecosystem goods and services such as carbon sequestration, habitat for terrestrial and marine fauna, and coastal hazard mitigation. The use of satellite remote sensing to map mangroves has become widespread as it can provide accurate, efficient, and repeatable assessments. Traditional remote sensing approaches have failed to accurately map fringe mangroves and true mangrove species due to relatively coarse spatial resolution and/or spectral confusion with landward vegetation. This study demonstrates the use of the new Worldview-2 sensor, Object-based image analysis (OBIA), and support vector machine (SVM) classification to overcome both of these limitations. An exploratory spectral separability showed that individual mangrove species could not be spectrally separated, but a distinction between true and associate mangrove species could be made. An OBIA classification was used that combined a decision-tree classification with the machine-learning SVM classification. Results showed an overall accuracy greater than 94% (kappa = 0.863) for classifying true mangroves species and other dense coastal vegetation at the object level. There remain serious challenges to accurately mapping fringe mangroves using remote sensing data due to spectral similarity of mangrove and associate species, lack of clear zonation between species, and mixed pixel effects, especially when vegetation is sparse or degraded.
    Electronic ISSN: 2072-4292
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Published by MDPI Publishing
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  • 13
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    Satellite remote sensing of mangrove forests: Recent advances and future opportunities (2011)
    Sage
    Details
    Publication Date: 2011-02-02
    Description: Mangroves are salt tolerant woody plants that form highly productive intertidal ecosystems in tropical and subtropical regions. Despite the established importance of mangroves to the coastal environment, including fisheries, deforestation continues to be a major threat due to pressures for wood and forest products, land conversion to aquaculture, and coastal urban development. Over the past 15 years, remote sensing has played a crucial role in mapping and understanding changes in the areal extent and spatial pattern of mangrove forests related to natural disasters and anthropogenic forces. This paper reviews recent advancements in remote-sensed data and techniques and describes future opportunities for integration or fusion of these data and techniques for large-scale monitoring in mangroves as a consequence of anthropogenic and climatic forces. While traditional pixel-based classification of Landsat, SPOT, and ASTER imagery has been widely applied for mapping mangrove forest, more recent types of imagery such as very high resolution (VHR), Polarmetric Synthetic Aperture Radar (PolSAR), hyperspectral, and LiDAR systems and the development of techniques such as Object Based Image Analysis (OBIA), spatial image analysis (e.g. image texture), Synthetic Aperture Radar Interferometry (InSAR), and machine-learning algorithms have demonstrated the potential for reliable and detailed characterization of mangrove forests including species, leaf area, canopy height, and stand biomass. Future opportunities include the application of existing sensors such as the hyperspectral HYPERION, the application of existing methods from terrestrial forest remote sensing, investigation of new sensors such as ALOS PRISM and PALSAR, and overcoming challenges to the global monitoring of mangrove forests such as wide-scale data availability, robust and consistent methods, and capacity-building with scientists and organizations in developing countries.
    Print ISSN: 0309-1333
    Electronic ISSN: 1477-0296
    Topics: Geography
    Published by Sage
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  • 14
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    Sterols in soil organic matter in relation to nitrogen mineralization in sandy arable soils (2011)
    Wiley
    Details
    Publication Date: 2011-03-22
    Description: Unusually low net N mineralization in soils relatively rich in total organic C and N was repeatedly reported for sandy arable soils in NW Europe. In order to adequately account for it in simulation models, it is necessary to know the involved substances and processes. Therefore, 9 arable top soils (〈 6% clay) with a wide range of total organic C (1.1%–5.2%) and C : N ratios (12–35) were studied. The soils varied strongly in the mineralizability of soil organic N which was determined via long-term laboratory incubations (〉 200 d). It was hypothesized that mineralization was controlled by antioxidants, and the Trolox equivalent antioxidant capacity (TEAC) of the soils was measured. In addition, pyrolysis–field ionization mass spectrometry (Py-FIMS) was applied to investigate the influence of the molecular-chemical composition of soil organic matter. In these soils, the compound class of sterols from Py-FIMS analysis was most closely, negatively correlated with the mineralizability of soil organic N ( r 〈?h.3〉 2 = 0.75, p = 0.003). This was probably not an antioxidative effect, because the TEAC values did not correlate sufficiently with the mineralizability and the sterol intensities. However, the negative relation with sterols could be causal, since the correlation was about as close with other components of the compound class of sterols and even closer with the main plant sterol beta-sitosterol ( r 〈?h.3〉 2 = 0.84, p = 0.001). In addition, the variability among samples was strongly governed by the proportions of sterols, and sterols also had a high discriminating power in discriminant analysis. Furthermore, the proportions of sterols were extraordinary in those arable podzol soils that developed under previous heath- or woodland (up to 10.2% of total ion intensity from Py-FIMS). In conclusion, the inhibitory effect of these compounds needs to be investigated in more detail in order to optimize parameterization of N as well as C simulation models especially for podzolized, sandy arable soils with former heath- or woodland vegetation.
    Print ISSN: 1436-8730
    Electronic ISSN: 1522-2624
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 15
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    Polyhedral membrane protein proteoliposomes [Biophysics and Computational Biology] (2014)
    National Academy of Sciences
    Details
    Publication Date: 2014-01-15
    Description: We demonstrate that membrane proteins and phospholipids can self-assemble into polyhedral arrangements suitable for structural analysis. Using the Escherichia coli mechanosensitive channel of small conductance (MscS) as a model protein, we prepared membrane protein polyhedral nanoparticles (MPPNs) with uniform radii of ∼20 nm. Electron cryotomographic analysis established that these MPPNs...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 16
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    Mapping of catalytic residues in the RNA polymerase active center (1996)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1996-07-05
    Description: When the Mg2+ ion in the catalytic center of Escherichia coli RNA polymerase (RNAP) is replaced with Fe2+, hydroxyl radicals are generated. In the promoter complex, such radicals cleave template DNA near the transcription start site, whereas the beta' subunit is cleaved at a conserved motif NADFDGD (Asn-Ala-Asp-Phe-Asp-Gly-Asp). Substitution of the three aspartate residues with alanine creates a dominant lethal mutation. The mutant RNAP is catalytically inactive but can bind promoters and form an open complex. The mutant fails to support Fe2+-induced cleavage of DNA or protein. Thus, the NAD-FDGD motif is involved in chelation of the active center Mg2+.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zaychikov, E -- Martin, E -- Denissova, L -- Kozlov, M -- Markovtsov, V -- Kashlev, M -- Heumann, H -- Nikiforov, V -- Goldfarb, A -- Mustaev, A -- New York, N.Y. -- Science. 1996 Jul 5;273(5271):107-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Limnological Institute, Russian Academy of Sciences, Irkutsk, Russia.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8658176" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Aspartic Acid/metabolism ; Binding Sites ; DNA/metabolism ; DNA-Directed RNA Polymerases/*chemistry/genetics/*metabolism ; Dithiothreitol/pharmacology ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli/*enzymology ; Ferrous Compounds/metabolism ; Hydroxyl Radical ; Magnesium/metabolism ; Molecular Sequence Data ; Mutagenesis ; Promoter Regions, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 17
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    Prostratin analogs induce latent HIV expression [Medical Sciences] (2013)
    National Academy of Sciences
    Details
    Publication Date: 2013-07-17
    Description: Highly active antiretroviral therapy (HAART) decreases plasma viremia below the limits of detection in the majority of HIV-infected individuals, thus serving to slow disease progression. However, HAART targets only actively replicating virus and is unable to eliminate latently infected, resting CD4+ T cells. Such infected cells are potentially capable of...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 18
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    Remote Sensing, Vol. 3, Pages 2440-2460: An Object-Based Classification of Mangroves Using a Hybrid Decision Tree—Support Vector Machine Approach (2012)
    MDPI Publishing
    Details
    Publication Date: 2012-05-03
    Description: Mangroves provide valuable ecosystem goods and services such as carbon sequestration, habitat for terrestrial and marine fauna, and coastal hazard mitigation. The use of satellite remote sensing to map mangroves has become widespread as it can provide accurate, efficient, and repeatable assessments. Traditional remote sensing approaches have failed to accurately map fringe mangroves and true mangrove species due to relatively coarse spatial resolution and/or spectral confusion with landward vegetation. This study demonstrates the use of the new Worldview-2 sensor, Object-based image analysis (OBIA), and support vector machine (SVM) classification to overcome both of these limitations. An exploratory spectral separability showed that individual mangrove species could not be spectrally separated, but a distinction between true and associate mangrove species could be made. An OBIA classification was used that combined a decision-tree classification with the machine-learning SVM classification. Results showed an overall accuracy greater than 94% (kappa = 0.863) for classifying true mangroves species and other dense coastal vegetation at the object level. There remain serious challenges to accurately mapping fringe mangroves using remote sensing data due to spectral similarity of mangrove and associate species, lack of clear zonation between species, and mixed pixel effects, especially when vegetation is sparse or degraded.
    Electronic ISSN: 2072-4292
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Published by MDPI Publishing
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  • 19
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    Bisphenol A Binds to Ras Proteins and Competes with Guanine Nucleotide Exchange: Implications for GTPase-Selective Antagonists (2013)
    American Chemical Society (ACS)
    Details
    Publication Date: 2013-11-24
    Description: Journal of Medicinal Chemistry DOI: 10.1021/jm401291q
    Topics: Chemistry and Pharmacology
    Published by American Chemical Society (ACS)
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  • 20
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    Geosciences, Vol. 3, Pages 543-584: The Lost South Gobi Microcontinent: Protolith Studies of Metamorphic Tectonites and Implications for the Evolution of Continental Crust in Southeastern Mongolia (2013)
    MDPI Publishing
    Details
    Publication Date: 2013-08-22
    Description: The Central Asian Orogenic Belt, or Altaids, is an amalgamation of volcanic arcs and microcontinent blocks that records a complex late Precambrian–Mesozoic accretionary history. Although microcontinents cored by Precambrian basement are proposed to play an integral role in the accretion process, a lack of isotopic data hampers volume estimates of newly produced arc-derived versus old-cratonic crust in southeastern Mongolia. This study investigates metamorphic tectonites in southern Mongolia that have been mapped as Precambrian in age, largely on the basis of their high metamorphic grade and high strain. Here we present results from microstructural analyses and U-Pb zircon geochronology on samples from Tavan Har (44.05° N, 109.55° E) and the Yagan-Onch Hayrhan metamorphic core complex (41.89° N, 104.24° E). Our results show no compelling evidence for Precambrian basement in southeastern Mongolia. Rather, the protoliths to all tectonites examined are Paleozoic–Mesozoic age rocks, formed during Devonian–Carboniferous arc magmatism and subsequent Permian–Triassic orogenesis during collision of the South Mongolia arc with the northern margin of China. These results yield important insights into the Paleozoic accretionary history of southern Mongolia, including the genesis of metamorphic and igneous basement during the Paleozoic, as well as implications for subsequent intracontinental reactivation.
    Electronic ISSN: 2076-3263
    Topics: Geosciences
    Published by MDPI Publishing
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