ISSN:
0741-0581
Schlagwort(e):
Vascular cell cultures
;
Transmission electron microscopy
;
Life and Medical Sciences
;
Cell & Developmental Biology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Allgemeine Naturwissenschaft
Notizen:
A method is described for obtaining optimal, reproducible ultrastructure of vascular smooth muscle cells and vascular endothelial cells in culture. Routinely grown cultures are prepared for TEM with a precise regimen of fixation, postfixation, en bloc staining, dehydration, and embedment. The most important aspects of this procedure are the following: (1) fixation with a percentage-gradient series of glutaraldehyde solutions at 37°C, (2) immediate postfixation with osmium tetroxide solution, and (3) block-staining with uranyl acetate solution to eliminate any extraction of constituents during subsequent processing.
Zusätzliches Material:
8 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/jemt.1060010305
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