Publikationsdatum:
2016-01-28
Beschreibung:
To investigate gene specificity at the level of translation in both the human genome and viruses, we devised a high-throughput bicistronic assay to quantify cap-independent translation. We uncovered thousands of novel cap-independent translation sequences, and we provide insights on the landscape of translational regulation in both humans and viruses. We find extensive translational elements in the 3' untranslated region of human transcripts and the polyprotein region of uncapped RNA viruses. Through the characterization of regulatory elements underlying cap-independent translation activity, we identify potential mechanisms of secondary structure, short sequence motif, and base pairing with the 18S ribosomal RNA (rRNA). Furthermore, we systematically map the 18S rRNA regions for which reverse complementarity enhances translation. Thus, we make available insights into the mechanisms of translational control in humans and viruses.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Weingarten-Gabbay, Shira -- Elias-Kirma, Shani -- Nir, Ronit -- Gritsenko, Alexey A -- Stern-Ginossar, Noam -- Yakhini, Zohar -- Weinberger, Adina -- Segal, Eran -- New York, N.Y. -- Science. 2016 Jan 15;351(6270). pii: aad4939. doi: 10.1126/science.aad4939. Epub 2016 Jan 14.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel. Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel. ; The Delft Bioinformatics Laboratory, Department of Intelligent Systems, Delft University of Technology, Delft, Netherlands. Platform Green Synthetic Biology, Delft, Netherlands. Kluyver Centre for Genomics of Industrial Fermentation, Delft, Netherlands. ; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel. ; Department of Computer Science, Technion, Haifa, Israel. Agilent Laboratories, Tel-Aviv, Israel. ; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel. Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel. eran.segal@weizmann.ac.il.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26816383" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
3' Untranslated Regions/genetics
;
5' Untranslated Regions/genetics
;
Base Pairing
;
Gene Expression Regulation, Viral
;
Genome, Human/*genetics
;
Genome, Viral/*genetics
;
High-Throughput Nucleotide Sequencing/methods
;
Humans
;
Internal Ribosome Entry Sites/genetics
;
Mutagenesis
;
Oligonucleotides/chemistry/genetics
;
Protein Biosynthesis/*genetics
;
RNA Caps/*genetics
;
RNA Viruses/genetics
;
RNA, Ribosomal, 18S/genetics/metabolism
;
Sequence Analysis, DNA/methods
;
Sequence Analysis, RNA/methods
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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