Publikationsdatum:
2012-10-24
Beschreibung:
Systematic analysis of the RNA-protein interactome requires robust and scalable methods. We here show the combination of two completely orthogonal, generic techniques to identify RNA-protein interactions: PAR-CLIP reveals a collection of RNAs bound to a protein whereas SILAC-based RNA pull-downs identify a group of proteins bound to an RNA. We investigated binding sites for five different proteins (IGF2BP1-3, QKI and PUM2) exhibiting different binding patterns. We report near perfect agreement between the two approaches. Nevertheless, they are non-redundant, and ideally complement each other to map the RNA-protein interaction network.
Schlagwort(e):
RNA characterisation and manipulation
Print ISSN:
0305-1048
Digitale ISSN:
1362-4962
Thema:
Biologie
Permalink
