Digitale Medien
Oxford, UK
:
Blackwell Publishing Ltd
FEMS microbiology letters
37 (1986), S. 0
ISSN:
1574-6968
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Biologie
Notizen:
Abstract HPA proteolytic assay was used to study the dependence of proteolysis on phosphorus in fresh water. Inorganic phosphate (Pi) stimulated the growth of bacteria producing proteases in water when P was limiting factor, but did not affect the biochemical activity of enzymes which were P-in-dependent. In proteolytic assays, bacteria utilised nitrogen and carbon from HPA. Therefore, during long incubations, significant increases in microbial biomass were observed. The original HPA procedure [12] gives artificial and non-realistic values of proteolytic rates in aquatic habitats due to accelerated and uncontrolled bacterial growth and enzyme production during the assay. The use of toluene to prevent microbial growth in HPA assays is recommended [10].
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1574-6968.1986.tb01793.x
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