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  • 1
    Digitale Medien
    Digitale Medien
    Isolation, Purification, and Characterization of Lipase Isoenzymes from a Technical Aspergillus niger Enzyme (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 50 (1985), S. 0 
    Details
    ISSN: 1750-3841
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A qualitative screening revealed the occurrence of lipase, esterase, protease, amylase, endo-1,4-β-D-glucanase, xylanase, pectinmethylesterase, polygalacturonase, catalase, β-D-glucosidase and β-D-galactosidase activities in the technical Aspergillus niger enzyme under study (Lipase 2212 D, Röhm). The isolation and purification of lipolytic activities were performed by combination of DEAE-Trisacryl M ion exchange chromatography, Sephadex G 50 gel filtration and hydrophobic chromatography using Phenylsepharose CL-4B. The individual purification steps were checked by specific enzyme visualization in ultrathin agar gels after ultrathin-layer isoelectric focusing (UIEF). Two UIEF homogeneous lipase isoenzymes (I and II) were isolated and characterized by the following parameters: isoelectric points (I: 4.0; II. 3.5); molecular weights (I: 31000 daltons; II: 19000 daltons); carbohydrate contents (I: 6%; II: 9%) and compositions; pH optima (I, II: 5-6); substrate specificities and various effectors.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2621.1985.tb10574.x
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 2
    Digitale Medien
    Digitale Medien
    Fecundity of whitefish (Coregonus lavaretus) during the eu-and oligotrophication of Lake Constance (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 43 (1993), S. 0 
    Details
    ISSN: 1095-8649
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: About 20 ovaries of the whitefish ‘Blaufelchen’ (Coregonus lavaretus L.) of Lake Constance (Bodensee) were collected annually from 1964-1991. Absolute and relative fecundity peaked in the early 1980s, lagging about 2 years behind the maximum of total-P in the water (during the circulation period). Out of the biological time-series of Lake Constance, whitefish fecundity is the only one known to follow the phosphorus curve, and this may be the first time-series documented case of parallel trend reversal in the trophic state of a lake and fish biology. It is concluded that fish fecundity serves, at best, as an unspecific monitor of the overall well-being of the fish.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1095-8649.1993.tb00412.x
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 3
    Digitale Medien
    Digitale Medien
    Crustal structure of the Lofoten continental margin, off northern Norway, from ocean-bottom seismographic studies (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 121 (1995), S. 0 
    Details
    ISSN: 1365-246X
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Geologie und Paläontologie
    Notizen: In 1988, an extensive geophysical experiment was performed on the Lofoten passive continental margin, which was formed by the continental break-up between Greenland and northern Norway at 58 Ma. The geological units of the outer Lofoten margin are characterized by seaward-dipping reflectors (SDRs) and landward flood basalt, which extends up to 100 km landwards of SDRs. In this study, we obtain the P-wave velocity structure beneath the Lofoten Basin, the SDRs, and the landward flood basalt by use of ocean-bottom seismograph refraction profiling, and we also discuss the formation of the northern Norwegian passive continental margin.In the Lofoten Basin the crust is of oceanic type, consisting of sedimentary layers, oceanic laver 2 (4.9−5.5 km s−1), layer 3A (6.3−6.8 km s−1) and layer 3B (7.0−7.1 km s−1). Beneath the SDRs the crustal layers are identical to those of the Lofoten Basin, but the thickness of the lower crust, which represents the same velocity as layer 3B, increases to 5 km towards the continent side, and a high-velocity lower crustal layer (7.3 km s−1) is formed at the base of the crust. The ocean-continent transition zone is situated between the landward side of the SDRs and the northward continuation of the Vøring Plateau Escarpment. In this region the velocity of the lower crust gradually decreases and approaches the lower crustal velocity beneath the Lofoten Islands (6.8 km s−1). The model also indicates that the high-velocity layer disappears in this region. Comparing our model with the crustal structure on the Vøring margin, it is clear that the lower crustal body (≥7 km s−1) thickens southwards along the northern Norwegian continental margin. Recent results from petrological and geophysical studies of the generation of the oceanic crust have shown that increasing the temperature of the upwelling asthenospheric material increases the thickness of the oceanic crust. We interpret this as that the oceanic crust in the southern area in the Vøring-Lofoten margin was generated by hotter material than that of the northern area.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-246X.1995.tb06447.x
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 4
    Digitale Medien
    Digitale Medien
    Distribution of parvalbumin isotypes in adult snook and their potential applications as species-specific biomarkers (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 51 (1997), S. 0 
    Details
    ISSN: 1095-8649
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: The highly stable Ca2+ binding protein, parvalbumin, is prevalent in fish white muscle tissue. The properties of this protein make it a promising antigen for use as a specific biomarker for fish identification. Parvalbumin was purified from white muscle of an adult common snook Centropomus undecimalis using ammonium sulfate precipitation, size-exclusion chromatography (SEC) and anion-exchange HPLC. Parvalbumins were characterized by the presence of an 11-kDa band following gradient-SDS gel electrophoresis and by their immunoreactivity against mouse anti-parvalbumin antibodies. Anion-exchange chromatography of the parvalbumin fraction separated from the SEC column yielded nine fractions. Subsequent analysis of these fractions by isoelectric focusing gel electrophoresis led to a total of seven parvalbumin isotypes, which may lend themselves as biomarkers in fish identification. The presence of these seven parvalbumin isotypes was confirmed independently by reversed-phase HPLC. A dilution endpoint immunoassay was developed for C. undecimalis parvalbumin using a monoclonal antibody directed against its highly conserved calcium binding site. The utility of parvalbumin isotype distribution and specific monoclonal antibodies against fish parvalbumin in species identification is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1095-8649.1997.tb01512.x
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 5
    facet.materialart.
    Unbekannt
    Analytical Shear Statistics Based on Nocturnal Wind Profiles (1986)
    American Meteorological Society
    Details
    Publikationsdatum: 1986-11-01
    Print ISSN: 0733-3021
    Digitale ISSN: 2163-5366
    Thema: Geologie und Paläontologie , Physik
    Publiziert von American Meteorological Society
    Standort Signatur Erwartet Verfügbarkeit
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    AKTUELLE ARTIKEL
  • 6
    facet.materialart.
    Unbekannt
    Atmospheric and Surface Processes, and Feedback Mechanisms Determining Arctic Amplification: A Review of First Results and Prospects of the (AC)3 Project (2023)
    American Meteorological Society
    In:  EPIC3Bulletin of the American Meteorological Society, American Meteorological Society, 104(1), pp. e208-e242, ISSN: 0003-0007
    Details
    Publikationsdatum: 2023-04-26
    Beschreibung: Mechanisms behind the phenomenon of Arctic amplification are widely discussed. To contribute to this debate, the (AC)3 project was established in 2016 (www.ac3-tr.de/). It comprises modeling and data analysis efforts as well as observational elements. The project has assembled a wealth of ground-based, airborne, shipborne, and satellite data of physical, chemical, and meteorological properties of the Arctic atmosphere, cryosphere, and upper ocean that are available for the Arctic climate research community. Short-term changes and indications of long-term trends in Arctic climate parameters have been detected using existing and new data. For example, a distinct atmospheric moistening, an increase of regional storm activities, an amplified winter warming in the Svalbard and North Pole regions, and a decrease of sea ice thickness in the Fram Strait and of snow depth on sea ice have been identified. A positive trend of tropospheric bromine monoxide (BrO) column densities during polar spring was verified. Local marine/biogenic sources for cloud condensation nuclei and ice nucleating particles were found. Atmospheric–ocean and radiative transfer models were advanced by applying new parameterizations of surface albedo, cloud droplet activation, convective plumes and related processes over leads, and turbulent transfer coefficients for stable surface layers. Four modes of the surface radiative energy budget were explored and reproduced by simulations. To advance the future synthesis of the results, cross-cutting activities are being developed aiming to answer key questions in four focus areas: lapse rate feedback, surface processes, Arctic mixed-phase clouds, and airmass transport and transformation.
    Repository-Name: EPIC Alfred Wegener Institut
    Materialart: Article , isiRev
    Format: application/pdf
    Standort Signatur Erwartet Verfügbarkeit
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