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  • 2000-2004  (138)
  • 1910-1914
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  • 1
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    Phospholipids in ODP Leg 190 samples (Table 2) (2003)
    PANGAEA
    In:  Supplement to: Zink, Klaus-Gerhard; Wilkes, Heinz; Disko, Ulrich; Elvert, Marcus; Horsfield, Brian (2003): Intact phospholipids - microbial life markers in marine deep subsurface sediments. Organic Geochemistry, 34(6), 755-769, https://doi.org/10.1016/S0146-6380(03)00041-X
    Details
    Publikationsdatum: 2024-01-09
    Beschreibung: Deep subsurface sediments from the Nankai Trough, Japan Sea, ODP Leg 190, sites 1173, 1174, 1177, and near-surface sediments from Hydrate Ridge, NE-Pacific have been analysed by high performance liquid chromatography (HPLC)–electrospray ionisation (ESI)-mass spectrometry (MS). The main objective was to utilize the presence of intact phospholipids as a direct indicator of viable microorganisms. The extracts of Nankai Trough sediments were found to contain a variety of phospholipid (PL) structures, well-known to stem from microorganisms, to depths as great as 745 mbsf and in situ temperatures as high as 85 °C. In addition, high relative amounts of lysophospholipids (e.g. lysophosphatidylcholines) exceeding those of the regular phospholipids were detected. Diglyceride mass fragments of various PLs have been assigned to fatty acyl side-chains of typical chain length (C14, C16, C18, C20) and degree of unsaturation (zero, one or two double bonds). Similar results were obtained for the phospholipid distribution in extracts of organic matter-rich Hydrate Ridge sediments. To date, the enhanced occurrence of lysophospholipids cannot be explained completely but a response to increasing thermal and ecological stress seems probable.
    Schlagwort(e): 190-1173A; 190-1174; 190-1177A; Carbon, organic, total; COMPCORE; Composite Core; DEPTH, sediment/rock; Description; DRILL; Drilling/drill rig; DSDP/ODP/IODP sample designation; Event label; Extract; Joides Resolution; Leg190; Ocean Drilling Program; ODP; Philippine Sea; Phospholipids, per unit mass total organic carbon; Phospholipids per unit sediment mass; Sample code/label; Sample mass
    Materialart: Dataset
    Format: text/tab-separated-values, 91 data points
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    DATEN PANGAEA
  • 2
    Digitale Medien
    Digitale Medien
    Specific identification and molecular typing analysis of Lactobacillus johnsonii by using PCR-based methods and pulsed-field gel electrophoresis (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 217 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: A fast and reliable Multiplex-PCR assay was established to identify the species Lactobacillus johnsonii. Two opposing rRNA gene-targeted primers have been designed for this specific PCR detection. Specificity was verified with DNA samples isolated from different lactic acid bacteria. Out of 47 Lactobacillus strains isolated from different environments, 16 were identified as L. johnsonii by PCR. The same set of strains was investigated with five alternative molecular typing methods: enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), repetitive extragenic palindromic PCR (REP-PCR), amplified fragment length polymorphism, single triplicate arbitrarily primed PCR, and pulsed-field gel electrophoresis in order to compare the discriminatory power of these methods. The reported data strongly support the highly significant heterogeneity among all L. johnsonii isolates, potentially linked to their origin of isolation. The use of species-specific primers as well as rapid and highly powerful PCR-based molecular typing tools (namely ERIC- and REP-PCR techniques) should be respectively envisaged for identifying, differentiating and monitoring L. johnsonii strains from various environmental samples, for product monitoring, for species tracing in clinical studies as well as bacterial profiling of various microecological or gastrointestinal environments.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11468.x
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  • 3
    Digitale Medien
    Digitale Medien
    Molecular analysis of ancient microbial infections (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 213 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: The detection of ancient microbial DNA offers a new approach for the study of infectious diseases, their occurrence, frequency and host–pathogen interaction in historic times and populations. Moreover, data obtained from skeletal and mummified tissue may represent an important completion of contemporary phylogenetic analyses of pathogens. In the last few years, a variety of bacterial, protozoal and viral infections have been detected in ancient tissue samples by amplification and characterization of specific DNA fragments. This holds particularly true for the identification of the Mycobacterium tuberculosis complex, which seems to be more robust than other microbes due to its waxy, hydrophobic and lipid-rich cell wall. These observations provided useful information about the occurrence, but also the frequency of tuberculosis in former populations. Moreover, these studies suggest new evolutionary models and indicate the route of transmission between human and animals. Until now, other pathogens, such as Mycobacterium leprae, Yersinia pestis, Plasmodium falciparum and others, have occasionally been identified – mostly in single case studies or small sample sizes – as well, although much less information is available on these pathogens in ancient settings. The main reason therefore seems to be the degradation and modification of ancient DNA by progressive oxidative damage. Furthermore, the constant risk of contamination by recent DNA forces to take time and cost effective measures and renders the analysis of ancient microbes difficult. Nevertheless, the study of microbial ancient DNA significantly contributes to the understanding of transmission and spread of infectious diseases, and potentially to the evolution and phylogenetic pathways of pathogens.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11298.x
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  • 4
    Digitale Medien
    Digitale Medien
    Chromodomains are protein–RNA interaction modules (2000)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 407 (2000), S. 405-409 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] In Drosophila, compensation for the reduced dosage of genes located on the single male X chromosome involves doubling their expression in relation to their counterparts on female X chromosomes. Dosage compensation is an epigenetic process involving the specific acetylation of histone ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/35030169
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  • 5
    Digitale Medien
    Digitale Medien
    Determination of relative growth rates of natural quartz crystals (2000)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 404 (2000), S. 865-869 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Although the theory describing crystal growth in the geological environment is well established, there are few quantitative studies that delimit the absolute time involved in the growth of natural crystals. The actual mechanisms responsible for the variation in size and shape of individual ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/35009091
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  • 6
    Digitale Medien
    Digitale Medien
    A mass spectrometer system for analysis of polar stratospheric aerosols (2002)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 73 (2002), S. 446-452 
    Details
    ISSN: 1089-7623
    Quelle: AIP Digital Archive
    Thema: Physik , Elektrotechnik, Elektronik, Nachrichtentechnik
    Notizen: A balloon-borne mass spectrometer system has been flown successfully to determine the chemical composition of polar stratospheric aerosols over northern Scandinavia. The experiment combines an aerodynamic lens which collimates the aerosols into a narrow beam, a small sphere in which they evaporate, and a mass spectrometer for gas analysis. High-speed differential pumping by two liquid helium pumps effectively lowers the presence of ambient gases without affecting the particles of the beam. Field measurements and aerosol studies inside a large cryo-chamber have shown that the concept of particle focusing, evaporation and subsequent mass spectrometric gas analysis is a reliable technique to determine the molecular composition of aerosols especially in polar stratospheric clouds. © 2002 American Institute of Physics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.1430732
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  • 7
    Digitale Medien
    Digitale Medien
    Thin film microcalorimeter for heat capacity measurements in high magnetic fields (2002)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 73 (2002), S. 1841-1844 
    Details
    ISSN: 1089-7623
    Quelle: AIP Digital Archive
    Thema: Physik , Elektrotechnik, Elektronik, Nachrichtentechnik
    Notizen: Si–N based membrane calorimeters are a promising technology for the study of thermal properties of small quantities of materials in both pulsed and steady-state magnetic fields to 60 T and beyond. We present results that demonstrate our ability to measure the heat capacity of thin film samples from 2–300 K in steady-state fields up to 8 T. These measurements include the magnetoresistance of the Pt and Nb–Si thermometers and focus on confirming that the thermal conductance of the Si–N membrane does not change significantly in magnetic fields. This means the thermal conductance needs to be measured only in zero field, reducing the measurement time in high field. This is particularly important for future measurements in fields up to 60 T. © 2002 American Institute of Physics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.1461874
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  • 8
    Digitale Medien
    Digitale Medien
    Agroterrorism: Issues of Reality (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 69 (2004), S. 0 
    Details
    ISSN: 1750-3841
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: : In the past, deliberate contamination of foods in the U.S. has been committed by individual criminals, disgruntled employees, or political activists with a narrow agenda. After September 11, 2001, every nation has had to consider a much wider range of food security issues. Food is a global commodity and nearly every country both exports and imports foods. Every nation must consider the security of its domestic production and look beyond its borders to its trading partners to assure the safety of its food supply. In the nearly 2 years since September 11, 2001, many steps have been taken to improve food security. New legislation has been enacted to strengthen the ability of government to respond to terrorist threats. New relationships have been forged between food security agencies, law enforcement, and the intelligence community. Virtually every segment of the domestic and imported food supply has come under scrutiny in an effort to identify points of weakness and appropriate protective measures. Gaps in our knowledge about specific agents and food processes have been identified and research to fill these gaps has been initiated. This effort will continue for the foreseeable future and could have a wide range of benefits, including improvements to food safety, a reduction in product counterfeiting, and a reduction in the “gray market” food trade. The scale and diversity of the U.S. food supply makes it an attractive target, but at the same time makes it very resilient and responsive to emerging threats.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2621.2004.tb15492.x
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  • 9
    Digitale Medien
    Digitale Medien
    Molecular microbial analysis of Bifidobacterium isolates from different environments by the species-specific amplified ribosomal DNA restriction analysis (ARDRA) (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 36 (2001), S. 0 
    Details
    ISSN: 1574-6941
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: One hundred and six isolates of the genus Bifidobacterium, isolated from different environments (mainly gastrointestinal), were identified and classified taxonomically to species level by amplified ribosomal DNA restriction analysis. Two restriction endonucleases (Sau3AI and BamHI) were chosen for aligning the 16S rRNA sequences of 16 bifidobacterial species retrieved from various databases, to obtain species-specific restriction patterns. A rapid and accurate identification scheme was obtained by comparing the resulting 16S rDNA digestion profiles of 16 Bifidobacterium type-strains and 90 strains of various origins. All of the investigated strains were previously confirmed at the species level as belonging to the genus Bifidobacterium by fluorescence in-situ hybridisation and by polymerase chain reaction amplification with genus- and species-specific primers. The present work demonstrates that species-specific detection of Bifidobacterium adolescentis, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium catenulatum, Bifidobacterium coryneforme, Bifidobacterium cuniculi, Bifidobacterium dentium, Bifidobacterium infantis, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium suis, Bifidobacterium magnum, Bifidobacterium pseudolongum, Bifidobacterium pseudocatenulatum and Bifidobacterium pullorum present in different micro-ecological environments (e.g. gastrointestinal tract) can be accomplished in a reliable, rapid and accurate manner, circumventing the recognised deficiencies of traditional identification techniques.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6941.2001.tb00831.x
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  • 10
    Digitale Medien
    Digitale Medien
    Condensation en von Naphthalaldehydsäure mit Aceton und Acetophenon (1911)
    Springer
    Monatshefte für Chemie 32 (1911), S. 813-842 
    Details
    ISSN: 1434-4475
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01517733
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