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  • 1
    Digitale Medien
    Digitale Medien
    Development of pre-isogenic lines for rice blast-resistance by marker-aided selection from a recombinant inbred population (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 560-567 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Disease resistance ; Rice blast ; RFLPs ; Recombinant inbred lines ; Pre-isogenic lines
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract To increase the available set of near-isogenic lines (NILs) for blast-resistance in rice, we have developed a general method for establishing NILs from populations of fixed recombinants that have been used for gene mapping. We demonstrated the application of this method by the selection of lines carrying genes from the rice cultivar Moroberekan. Moroberekan is a West African japonica cultivar that is considered to have durable resistance to rice blast. Multiple genes from Moroberekan conferring complete and partial resistance to blast have previously been mapped using a recombinant inbred (RI) population derived from a cross between Moroberekan and the highly and broadly susceptible indica cultivar CO39. To analyze individual blast-resistance genes, it is desirable to transfer them individually into a susceptible genetic background. This RI population, and the associated data sets on blast reaction and restriction fragment length polymorphism (RFLP) genotypes, were used for selection of lines likely to carry individual blast-resistance genes and a minimum number of chromosomal segments from Moroberekan. Because skewed segregation in the RI population favored CO39 (indica) alleles, resistant lines carrying 8.7–17.5% of Moroberekan alleles (the proportion expected after two or three backcrosses) could be selected. We chose three RI lines carrying different complete resistance genes to blast and two RI lines carrying partial resistance genes to blast as potential parents for the development of NILs. These lines were subjected to genetic analysis, which allowed clarification of some issues that could not be resolved during the initial gene-mapping study.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00417948
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  • 2
    Digitale Medien
    Digitale Medien
    Variability and genetics of tolerance for aluminum toxicity in rice (Oryza sativa L.) (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 738-744 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Aluminum toxicity ; Diallel analysis ; Genetics ; Rice ; Variability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A study was undertaken to investigate the variability among lowland rice cultivars and the mode of gene action of aluminum (Al) toxicity tolerance in rice. Pregerminated seeds were grown in a nutrient solution containing 30 ppm Al and in normal nutrient solution, and relative root length (RRL) was determined at the 14-day-old stage to characterize genotypes for tolerance. Sixty-two traditional rice cultivars grown on lowland acid sulfate soil areas of Asia and West Africa were tested. Tolerant varieties ‘Azucena’, ‘IRAT104’, and ‘Moroberekan’, moderately sensitive ‘IR29’ and ‘IR43’, and sensitive ‘IR45’ and ‘IR1552’ were used to investigate the genetics of tolerance by diallel analysis. Of the 62 cultivars tested, only 3 were found to be sensitive to A l toxicity. Among the tolerant cultivars identified, 11 (‘Siyam Kuning’, ‘Gudabang Putih’, ‘Siyam’, ‘Lemo’, ‘Khao Daeng’, ‘Siyamhalus’, ‘Bjm-12’, ‘Ketan’, ‘Seribu Gantang’, ‘Bayer Raden Rati’, and ‘Padi Kanji’) were found to possess higher levels of tolerance than the improved tolerant upland cultivar ‘IRAT104’. Diallel analysis revealed that high RRL is governed by both additive and dominance effects with a preponderance of additive effects. The trait exhibited partial dominance, and one group of genes was detected. Heritability was high, and environmenal effects were low. Findings suggest that when breeding for A1 toxicity tolerance, selection can be made in early generations. The pedigree method of breeding would be suitable. Combining ability analysis revealed the importance of both general combining ability (GCA) and specific combining ability (SCA) in the genetics of A1 toxicity tolerance in rice. GCA was more prevalent than SCA. Tolerant parens ‘Azucena’, ‘IRAT104’, and ‘Moroberekan’ were the best general combiners. The presence of reciprocal effects among crosses suggested the proper choice of parents in hybridization programs. Results indicated that ‘Azucena’, ‘IRAT 104’, and ‘Moroberekan’ should be used as the female in crosses for A1 toxicity tolerance.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224070
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Comparison of rice lines derived through anther culture and the pedigree method in relation to blast (Pyricularia grisea Sacc.) resistance (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 583-590 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words  Stable resistance  ;  Doubled haploids  ; Pyricularia grisea  ;  Breeding  ;  Categorical data analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract   Crosses were made between Fanny (highly susceptible to blast) and 11 cultivars differing in blast resistance. Using the pedigree method (PM) segregating generations were evaluated and selected for blast resistance. Via anther culture (AC), doubled-haploids were obtained from F1 plants and from F2 blast-susceptible plants. Pedigree and anther culture-derived lines were planted together and evaluated for blast resistance under rainfed conditions at the Santa Rosa Experiment Station, Villavicencio, Colombia. The principal objective was to compare PM and AC in terms of their efficiency in producing rice lines resistant to blast. Results of a stratified analysis showed an association between method and blast resistance. Results of the logit-model analysis showed that AC produced a significantly (P=0.0001) higher proportion of lines with initial blast resistance (leaf- and neck-blast reaction ≤4) than did PM across all cross types. Stable blast resistance was assessed based on field performance over 3 years. AC was superior to PM in generating stable resistance for only some cross types. Consequently, with a few exceptions, AC can be used as effectively as PM to develop rice cultivars resistant to blast, with savings in time and labor. Additionally, blast-resistant lines were obtained either by the pedigree method or by anther culture from crosses between blast-susceptible cultivars (Fanny/CICA4 and Fanny/Colombia1). This excludes somaclonal variation as a possible mechanism responsible for this resistance and suggests that a recombination of minor genes could have occurred and was fixed through either method. However, the stability of the resistance was greater in pedigree-derived lines. The implications of these findings for rice blast-resistance breeding are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050167
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Comparison of rice lines derived through anther culture and the pedigree method in relation to blast (Pyricularia grisea Sacc.) resistance (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 583-590 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Stable resistance ; Doubled haploids ; Pyricularia grisea ; Breeding ; Categorical data analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Crosses were made between Fanny (highly susceptible to blast) and 11 cultivars differing in blast resistance. Using the pedigree method (PM) segregating generations were evaluated and selected for blast resistance. Via anther culture (AC), doubled-haploids were obtained from F1 plants and from F2 blast-susceptible plants. Pedigree and anther culture-derived lines were planted together and evaluated for blast resistance under rainfed conditions at the Santa Rosa Experiment Station, Villavicencio, Colombia. The principal objective was to compare PM and AC in terms of their efficiency in producing rice lines resistant to blast. Results of a stratified analysis showed an association between method and blast resistance. Results of the logit-model analysis showed that AC produced a significantly (P=0.0001) higher proportion of lines with initial blast resistance (leaf- and neck-blast reaction ≤4) than did PM across all cross types. Stable blast resistance was assessed based on field performance over 3 years. AC was superior to PM in generating stable resistance for only some cross types. Consequently, with a few exceptions, AC can be used as effectively as PM to develop rice cultivars resistant to blast, with savings in time and labor. Additionally, blast-resistant lines were obtained either by the pedigree method or by anther culture from crosses between blast-susceptible cultivars (Fanny/CICA4 and Fanny/Colombial). This excludes somaclonal variation as a possible mechanism responsible for this resistance and suggests that a recombination of minor genes could have occurred and was fixed through either method. However, the stability of the resistance was greater in pedigree-derived lines. The implications of these findings for rice blast-resistance breeding are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224562
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Development of pre-isogenic lines for rice blast-resistance by marker-aided selection from a recombinant inbred population (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 560-567 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Disease resistance ; Rice blast ; RFLPs ; Recombinant inbred lines ; Pre-isogenic lines
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract To increase the available set of near-isogenic lines (NILs) for blast-resistance in rice, we have developed a general method for establishing NILs from populations of fixed recombinants that have been used for gene mapping. We demonstrated the application of this method by the selection of lines carrying genes from the rice cultivar Moroberekan. Moroberekan is a West African japonica cultivar that is considered to have durable resistance to rice blast. Multiple genes from Moroberekan conferring complete and partial resistance to blast have previously been mapped using a recombinant inbred (RI) population derived from a cross between Moroberekan and the highly and broadly susceptible indica cultivar CO39. To analyze individual blast-resistance genes, it is desirable to transfer them individually into a susceptible genetic background. This RI population, and the associated data sets on blast reaction and restriction fragment length polymorphism (RFLP) genotypes, were used for selection of lines likely to carry individual blast-resistance genes and a minimum number of chromosomal segments from Moroberekan. Because skewed segregation in the RI population favored CO39 (indica) alleles, resistant lines carrying 8.7–17.5% of Moroberekan alleles (the proportion expected after two or three backcrosses) could be selected. We chose three RI lines carrying different complete resistance genes to blast and two RI lines carrying partial resistance genes to blast as potential parents for the development of NILs. These lines were subjected to genetic analysis, which allowed clarification of some issues that could not be resolved during the initial gene-mapping study.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050315
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 1121-1128 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words RFLP markers ; Fine-mapping ; Blast resistance ; Pyramiding ; Marker-assisted selection
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Three major genes (Pi1, Piz-5 and Pita) for blast resistance on chromosomes 11, 6 and 12, respectively, were fine-mapped and closely linked RFLP markers identified. New markers for Pi1 and Pita were found that were flanking the genes. The three genes were pyramided using RFLP markers. A PCR-based SAP (sequence amplified polymorphism) marker was used to identify Piz-5 in the segregating population. The plants carrying the two- and three-gene combinations that were tested for resistance to leaf blast in the Philippines and India indicated that combinations including Piz-5 have enhanced resistance than when it is present alone. The genes from the pyramided lines are at present being deployed into agronomically superior ricevarieties by marker-aided selection (MAS).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051395
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  • 7
    Digitale Medien
    Digitale Medien
    Improving sustainable productivity in rice-based rainfed lowland systems of South and Southeast Asia (1995)
    Springer
    GeoJournal 35 (1995), S. 307-324 
    Details
    ISSN: 1572-9893
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geographie
    Notizen: Abstract The lowland rice-producing areas of the world may be classified first by water regime: those with a reliable and controlled external supply of water and a drainage system, theirrigated ricelands, and those depending solely on rainfall and runoff, therainfed lowlands. The latter are diverse and range from drought-prone lands to those subjected to flooding in excess of several meters during the growing season. Rice production in these areas hasn't really benefited from the technological advances that were so successful for irrigated rice, yet hundreds of millions of Asia's poorest people depend upon the rainfed lowlands for their livelihood. This paper presents a retionale for devoting resources to research for the rainfed environments of Asia, and examines the institutional context and the key constraints and opportunities for intervention. The research strategies directed towards increasing sustainable productivity in these areas are presented, including the unique challenges presented by environmental heterogeneity and variability.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00989138
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Reaction of incompatible isolate of Pyricularia grisea on rice leaves stripped of epicuticular wax reflects blast conduciveness of soils (1995)
    Springer
    Plant and soil 170 (1995), S. 279-286 
    Details
    ISSN: 1573-5036
    Schlagwort(e): disease resistance screening ; leaf cuticle ; rice blast ; rice blast disease resistance ; upland rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Upland rice cultivars were evaluated in the greenhouse for susceptibility to the rice blast disease caused by Pyricularia grisea Sacc., on two upland soils from the Philippines previously considered to be “blast conducive” and “blast non-conducive”. Under monocyclic inoculation tests plants grown in conducive soil showed significantly greater lesion development than plants of the same cultivar grown in non-conducive soil: cultivars considered to be susceptible to the isolates used showed increased number of susceptible-type lesions; resistant cultivars showed increased number of hypersensitive resistant-type lesions. A similar effect was observed under polycyclic tests where several generations of the pathogen were allowed to develop on the test plants. Dilution of conducive soil with non-conducive soil resulted in a corresponding reduction of disease severity, although this was most pronounced on resistant cultivars. Removal of leaf epicuticular waxes (LEW) using organic solvents increased the number of resistant-type lesions on resistant cultivars grown in both soils following inoculation. Susceptible plants were not suitable for quantifying the relative blast conduciveness of a soil because of the extreme environmental sensitivity of the bioassay and the tendency of lesions to coalesce. Comparing numbers of resistant-type lesions on leaves of plants stripped of LEW and inoculated with an incompatible P. grisea isolate among plants grown in different soils proved to be a satisfactory means of distinguishing the relative blast conduciveness of soils under controlled conditions. This method was field tested in eastern India and results corroborated farmer assessment of which soils were blast conducive. Using incompatible isolate-cultivar combinations and LEW-free leaves is proposed as a simple bioassay for assessing blast conduciveness of soils and should prove useful in regional characterization of rice blast risk.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00010480
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  • 9
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    Improving sustainable productivity in rice-based rainfed lowland systems of South and Southeast Asia (1995)
    Springer
    Details
    Publikationsdatum: 1995-03-01
    Print ISSN: 0343-2521
    Digitale ISSN: 1572-9893
    Thema: Geographie
    Publiziert von Springer
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    facet.materialart.
    Unbekannt
    Reaction of incompatible isolate of Pyricularia grisea on rice leaves stripped of epicuticular wax reflects blast conduciveness of soils (1995)
    Springer
    Details
    Publikationsdatum: 1995-03-01
    Print ISSN: 0032-079X
    Digitale ISSN: 1573-5036
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Publiziert von Springer
    Standort Signatur Erwartet Verfügbarkeit
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