ISSN:
1750-3841
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
Triose phosphate isomerase (TPI) was purified from bovine semimembranosus muscle using acetone precipitation, ammonium sulfate precipitation and cation exchange chormatography. Anti-TPI polyclonal antibodies (PAbs) were raised in rabbits. A sandwich ELISA with a limit of detection of 1 μg TPI/mL was developed using PAbs for capture and biotinylated PAbs for detection. Cross-reactivities of antibodies with other protein in beef muscle extracts and with TPI from different animal species were examined. TPI concentration and activity of ground beef decreased (P 〈 0.0001) as cooking temperature was increased from 48.9 to 76.7°C, suggesting that these assays might be used to monitor processing adequacy of beef products.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1365-2621.1999.tb15097.x
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