ISSN:
1574-6968
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Biologie
Notizen:
Abstract Using an antibody raised against d-glucose dehydrogenase (EC 1.1.99.17) purified from Pseudomonas fluorescens, immuno-cross-reactivity with the enzymes from several bacterial strains and localization of the enzyme in Escherichia coli were examined. The antibody cross-reacted with glucose dehydrogenases from various Gram-negative bacteria examined. As a result, it became apparent that the enzymes from Gluconobacter, Acetobacter, Pseudomonas and Acinetobacter, which existed as holoenzymes in the membranes, had lower molecular weights than those from E. coli and Klebsiella, which were apoenzymes.Treatment with trypsin of right-side out and inside-out membrane vesicles from E. coli clearly demonstrated that d-glucose dehydrogenase was located on the outer surface of the cytoplasmic membrane of E. coli, as had been suggested for Pseudomonas.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1574-6968.1986.tb01782.x
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