Publikationsdatum:
2021-05-19
Beschreibung:
To investigate the effects of carvacrol supplementation in rainbow trout induced by
lipopolysaccharide (LPS), relative gene expressions levels of some pro-inflammatory
cytokines and apoptosis markers in kidney and liver were measured. Fish with a mean
weight of 44.71±1.33 g were studied using four different treatments with three
replicates each. Inflammation and apoptosis were performed using LPS of Escherichia
coli (25 µg ml
^-1
) except in the control group and only carvacrol (100 µg ml
^-1
diet)
containing diets were fed to this group (CAR). The last group was the infected fish fed
carvacrol supplemented diet (+CAR). Kidney and liver tissues were removed 3 days
after to determine the levels of interleukin-1β (IL-1β), interferon gamma (INF-γ),
caspase 3 (Cas 3), caspase 8 (Cas 8) using Real-Time PCR analyses. IL-1β expressions
of both kidney and liver was significantly decreased (12.9 and 2.14 fold, respectively)
in LPS treated cells (p〈0.05). While IFN-γ expression was up regulated in kidney, it
had down regulation in liver. LPS decreased both Cas 3 and 8 expressions in kidney but
increased in liver. +CAR increased expressions of IL-1β and IFN-γ compared with the
control (up to 2-3 fold) in all tissues except caspase gene expressions that were similar
in the control. In all tissues, IL-1β and IFN-γ expressions increased in +CAR group,
expect IFN-γ in the kidney. However, Cas 3 and 8 expressions including apoptosis was
induced by up-down regulations in all tissues compared to LPS-injected fish. The
results showed that carvacrol had pro-inflammatory and apoptotic effects especially on
liver tissue of LPS-induced inflammation model of Oncorhynchus mykiss.
Beschreibung:
Published
Schlagwort(e):
Rainbow trout
;
Oncorhynchus mykiss
;
LPS
;
Inflammatory cytokines
;
Caspases
;
Carvacrol
;
Feed
;
Kidney
;
Liver
;
Fish
Repository-Name:
AquaDocs
Materialart:
Journal Contribution
,
Refereed
Format:
pp.205-214
Permalink
