ISSN:
1075-4261
Schlagwort(e):
Chemistry
;
Analytical Chemistry and Spectroscopy
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Physik
Notizen:
Ytterbium (Yb3+) vibronic sideband (VSB) spectroscopy allows the identification of the lanthanide ligands both in model compounds and in Yb3+ -reconstituted metalloproteins. In some cases there exist multiple binding sites which complicate the Yb3+ fluorescence spectrum. The presence of different sites can be deduced from the variation of the intensity of the VBSs, when the wavelength of excitation is varied. A general two-dimensional (2D) correlation method developed by Noda can be potentially used to discriminate the different Yb3+ binding sites. A very simple procedure has been further developed so that, by using a few different spectra, usable information can be extracted from the 2D spectrum; the spectra were obtained with different wavelengths of excitation. This method is applied successfully in the present work on Yb3+-reconstituted transferrin, which contains two spectroscopically different Yb3+ sites, in order to determine any structural difference between the two sites. © 1995 John Wiley & Sons, Inc.
Zusätzliches Material:
4 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/bspy.350010504
Permalink